CRISPR Pooled Libraries基因编辑质粒文库试剂盒 BioVector NTCC质粒载体菌种细胞基因保藏中心

CRISPR Pooled Libraries基因编辑质粒文库试剂盒<br/></br>CRISPR Pooled Libraries基因编辑质粒文库试剂盒-BioVector NTCC Inc.</br></br>CRISPR is a useful tool for genetic screening experiments, due to the relative ease of designing gRNAs and the ability to modify virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing multiple gRNAs for each target gene. In a CRISPR screening experiment, target cells are treated with the pooled library to create a population of mutant cells that are then screened for a phenotype of interest. Screening experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic locus.</br>Before ordering, please review the steps for using pooled CRISPR libraries and keep the following in mind:</br>CRISPR screening experiments require electroporation to amplify the pooled library and the use of Next-Generation Sequencing (NGS) to confirm library representation.

CRISPR screening experiments require large quantities of cells and therefore, may not be suitable for use when the number of cells is limited (e.g. primary cells).

Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore, you must be equipped and authorized to use lentivirus in your lab.

Once you have determined that CRISPR-based screening is appropriate for your experiment, the next step is to pick a library. The pooled CRISPR libraries in BioVector’s collection differ based on some important criteria, and it is important to know which library best suits your particular experiment. Some important variables include:</br>Type of genetic modification - CRISPR libraries vary based on whether they are used to knockout, activate or repress target genes.

Number of gRNAs - A given CRISPR library may target the entire genome or a specific class of genes (subpools).

Species - the gRNAs within a given CRISPR library are unique to the genome of particular organism, and the library is only compatible with cells derived from that organism.

All of our current pooled CRISPR libraries can be found in the following table, which also includes information about the type of genetic modification and target species. Additional information about each library can be found on the individual library page.</br>Find Pooled Libraries of Interest

Click on different properties to create a custom filtered list of the CRISPR pooled libraries in BioVector's collection.</br>

Library Type</br> Activation

Barcode

Knockout

Inhibition

Species</br> Human

Mouse

Fly

T. gondii

E. coli

Library Size</br> Genome-wide

Subpool

Viral Prep Available </br> Yes

No

Name ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs

Activity-optimized genome-wide library Discontinued Knockout Human Sabatini and Lander 3rd 10 178,896

Activity-optimized genome-wide library 1000000100 Knockout Human Sabatini and Lander 3rd 10 187,535

Bassik Human CRISPR Knockout Library 101926 — 101934 Knockout Human Bassik 3rd 10 Varies

Bassik Mouse CRISPR Knockout Library 1000000121 — 1000000130 Knockout Mouse Bassik 3rd 10 Varies

Bikard Lab E. coli Genome-wide Inhibition Library v1 115927 Inhibition E. coli Bikard N/A 19 92,919

Bikard Lab EcoWG1: E. coli Genome-wide Inhibition Library v2 131625 Inhibition E. coli Bikard N/A ∼5 21,417

Bradley Human CRISPR Poison Exon Knockout Library 138084 Knockout Human Bradley 3rd ~9 pairs/exon 9,508

Broad GPP genome-wide Brunello 73179 (1 plasmid)

73178 (2 plasmid)  Knockout Human Doench and Root 3rd 4 76,441

Broad GPP genome-wide Brie 73632 (1 plasmid)

73633 (2 plasmid)  Knockout Mouse Doench and Root 3rd 4 78,637

Broad GPP kinome Brunello 75314, 75315

(1 plasmid)

75312, 75313

(2 plasmid) Knockout Human Doench and Root 3rd 4 3,052

Broad GPP kinome Brie 75317 (1 plasmid)

75316 (2 plasmid) Knockout Mouse Doench and Root 3rd 4 2,852

Broad GPP activation Calabrese p65-HSF 92379 (Set A)

92380 (Set B)  Activation Human Doench and Root 3rd 3–6 56,762 (Set A)

56,476 (Set B)

Broad GPP activation Caprano p65-HSF 92383 (Set A)

92384 (Set B) Activation Mouse Doench and Root 3rd 3–6 67,187 (Set A)

66,889 (Set B)

Broad GPP inhibition Dolcetto 92385 (Set A)

92386 (Set B) Inhibition Human Doench and Root 3rd 3–6 57,050 (Set A)

57,011 (Set B)

Broad GPP inhibition Dolomiti 104090 (Set A)

104091 (Set B) Inhibition Mouse Doench and Root 3rd 3–6 67,366 (Set A)

67,194 (Set B)

Cas13a/C2c2 Protospacer flanking site (PFS) Library 79153 Knockout E. coli Zhang N/A N/A - The protospacers contained in the library represent all 4096 (46) combinations of 6 nucleotides. N/A

Chong Zhang E. coli Genome-wide Inhibition Library 113134 — 113138

113146, 113147 Inhibition E. coli Chong Zhang N/A 15 Varies

CRiNCL - Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman 3rd 10 Varies

CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library 82480 Knockout Mouse Xie N/A N/A 395

CRISPRa Discontinued Activation Human Weissman 3rd 10 198,810

CRISPRa-v2 83978

1000000091 Activation Human Weissman 3rd 5

10 104,540

209,080

CRISPRa-v2 83996

1000000093 Activation Mouse Weissman 3rd 5

10 107,105

214,210

CRISPRi Discontinued Inhibition Human Weissman 3rd 10 206,421

CRISPRi-v2 83969

1000000090 Inhibition Human Weissman 3rd 5

10 104,535

209,070

CRISPRi-v2 83987

1000000092 Inhibition Mouse Weissman 3rd 5

10 107,415

214,830

Drosophila Cell CRISPR Knockout Library 134582

134583

134584 Knockout Fly Perrimon 3rd 8

6

6 7,956

17,827

59,406

Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout Human Sabatini and Lander 3rd 10 Varies

Focused Ras Synthetic Lethal Human CRISPR Knockout Library 92352 Knockout Human Sabatini and Lander 3rd 50 6,661

Human CRISPR Knockout Pooled Library (Gattinara) 136986 Knockout Human Doench 3rd 2 40,964

Mouse CRISPR Knockout Pooled Library (Gouda) 136987 Knockout Mouse Doench 3rd 2 44,155

hCRISPRa-v2 subpooled libraries 83980 — 83986 Activation Human Weissman 3rd 5 Varies

hCRISPRi-v2 subpooled libraries 83971 — 83977 Inhibition Human Weissman 3rd 5 Varies

mCRISPRa-v2 subpooled libraries 83998 — 84004 Activation Mouse Weissman 3rd 5 Varies

mCRISPRi-v2 subpooled libraries 83989 — 83995 Inhibition Mouse Weissman 3rd 5 Varies

Human CRISPRi titration libraries 136478 & 136479 Inhibition Mouse Weissman 3rd Varies Varies

Human CRISPR Knockout Library 1000000132 Knockout Human X.S. Liu 3rd 10 185,634

Human Epigenetic Knockout Library 162256 Knockout Human Birsoy 3rd 8 20,051

Human GeCKO v2 1000000048

(1 plasmid)

1000000049

(2 plasmid) Knockout Human Zhang 3rd 6 123,411

Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406

Human improved genome-wide library v1 67989 Knockout Human Yusa 3rd 5 90,709

Human Interferon-Stimulated Gene CRISPR Knockout Library 125753 Knockout Human Emerman

OhAinle 3rd 8 15,416

Human CRISPR lncRNA Activation Pooled Library 1000000106 Activation Human Zhang 3rd 10 96,458

Human CRISPR Metabolic Gene Knockout Library 110066 Knockout Human Sabatini 3rd 10 30,290

Human DNA Binding Domain-Focused CRISPR Knockout Library 123334 Knockout Human Vakoc 3rd 6 8,658

Human Genome-Wide Reduced Double-gRNA Library 137999 Knockout Human Parts 3rd 3 59,576

Human Whole Genome sgRNA iBAR Library 140633 Knockout Human Wei 3rd 3 58,630

Human Kinase Domain-Focused CRISPR Knockout Library 117725 Knockout Human Vakoc 3rd 6 3,051

Human lncRNA Splicing-targeting CRISPR Library 119977 Knockout Human Wei 3rd Varies 126,773

Human Membrane Protein Activation Library 113345 Activation Human Wright 3rd 7-14 58,570

Human miRNA CRISPR Knockout Library 112200 Knockout Human Lin 3rd 4-5 8,382

Human Paired-guide RNA (pgRNA) Library for Long Non-coding RNAs (lncRNAs) 89640 Knockout Human Wei 3rd Varies 12,472 pairs

Human SLC Activation Library 132561 Activation Human Superti-Furga 3rd 6 3,342

Human SLC Knockout Library 132552 Knockout Human Superti-Furga 3rd 6 2,609

Mini-human AsCpf1-based Human Genome-wide Knockout Library 130630 Knockout Human Draetta 3rd 3-4 17,032 arrays

Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library 138015 Knockout Mouse Pu N/A 6 14,671

Mouse GeCKO v2 1000000052

(1 plasmid)

1000000053

(2 plasmid) Knockout Mouse Zhang 3rd 6 130,209

Mouse genome-wide library v1 Discontinued Knockout Mouse Yusa 3rd 5 87,897

Mouse improved genome-wide library v2 67988 Knockout Mouse Yusa 3rd 5 90,230

Mouse CRISPR Metabolic Gene Knockout Library 160129 Knockout Mouse Birsoy 3rd 8 22,909

Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library 123361 Knockout Mouse Chen 3rd 4 11,934 arrays

Mouse SLC Knockout Library 141436 Knockout Mouse Superti-Furga 3rd 6 2,384

Mouse Toronto KnockOut (mTKO) CRISPR Library 159392

159393 Knockout Mouse Moffat 3rd ~5 19,463

Mouse Transcription Factor Activation Library 122131 Activation Mouse Qi 3rd 20 51,762

Mouse Tumor Suppressor Gene CRISPR Knockout Library 113584 (EFS)

113585 (TBG) Knockout Mouse Chen N/A (AAV) 4 286

Mouse Validation (mVAL) CRISPR Library 159391 Knockout Mouse Moffat 3rd 4 182

Neelamegham Human GlycoGene CRISPR Library 140961 Knockout Human Neelamegham 3rd 10 3,637

Oxford Fly 64750 Knockout D. melanogaster Liu N/A 3 40,279

Perturb-seq Guide Barcodes (GBC) 85968 Barcode Human Weissman 3rd N/A N/A

Retroviral Mouse Genome-wide CRISPR Knockout Library 104861 Knockout Mouse Teichmann N/A (retroviral) 5 90,230

RNA-Binding Protein Pooled CRISPR Knockout Library 141438 Knockout Human Yeo 3rd 10 12,472

SAM v1 - 3 plasmid system 1000000057 (Zeocin)

1000000074 (Puromycin) Activation Human Zhang 3rd 3 70,290

SAM v1 - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716

SAM v2 - 2 plasmid system 1000000078 (Blasticidin) Activation Human Zhang 3rd 3 70,290

CHyMErA Paralog & Dual-targeting hgRNA pooled library 155199 Knockout Human Moffat

Blencowe 3rd variable 92,746

CHyMErA Exon-deletion hgRNA pooled library 155200 Knockout Human Moffat

Blencowe 3rd variable 91,948

Toronto KnockOut - Version 1 1000000069 Knockout Human Moffat 3rd 12 176,500

Toronto KnockOut - Version 3 90294 (1 plasmid)

125517 (2 plasmid) Knockout Human Moffat 3rd 4 70,948

Toxoplasma Knockout 80636 Knockout T. gondii Lourido N/A 10 8,158

Two plasmid human activity-optimized genome-wide library 1000000095 Knockout Human Sabatini and Lander 3rd 10 187,536

Two plasmid mouse activity-optimized genome-wide library 1000000096 Knockout Mouse Sabatini and Lander 3rd 10 188,509<br/></br>Supplier来源:BioVector NTCC Inc.<br/>TEL电话:+86-010-53513060<br/>Website网址: http://www.biovector.net