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Fluorescent Protein Guide: Empty Backbones荧光报告质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

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Fluorescent Protein Guide: Empty Backbones荧光报告质粒载体<br/></br>Background

Fluorescent_Cells.jpg

Fluorescent proteins are genetically encoded tools that are used extensively by life scientists. The original green fluorescent protein (GFP) was cloned in 1992 (Prasher et al., Gene, 1992), and since then scientists have engineered numerous GFP-variants and non-GFP proteins that result in a diverse set of colors.</br>BioVector has assembled a collection of empty plasmid backbones with different fluorescent tags for you to create fusion proteins with your gene of interest. These are organized By Color of the fluorescent protein and By Expression Species .</br>The content for this page was generated with the help of Erik Snapp .

Empty Backbones for Fluorescent Protein Fusions (Organized by Color)

For each table:

Excitation and Emission maximum are listed in nm.

Brightness is the product of exctinction coefficient and quantum yield divided by 1000.

pKa is the pH at which fluorescence intensity is 50% of its maximum value.

Maturation is the time for fluorescence to obtain half-maximal value.

Structure describes the monomeric or oligomeric state of the protein. Many FPs are monomers at low concentration but are prone to dimerization at high concentration, so this is noted here. Proteins that are derived from GFP and contain the A206K mutation are monomeric at all concentrations, so this is mutation is noted when present.

For more information on the properties of many of these proteins, see:

Cranfill et al., Nature Methods 2016 Jul;13(7):557-62.

FPbase: A database for users of fluorescent proteins created by Talley Lambert at Harvard Medical School

More References below

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Blue/UV Cyan Green Yellow Orange Red Far-Red Near-Infrared

Long Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers</br>Blue/UV

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

Sirius 355 424 4 3.0  Prone to dimerization

Sirius-N1 - Mammalian Expression

SBFP2 380 446 13   Monomer (A206K)

pSBFP2-C1 - Mammalian Expression

Azurite 383 450 14 5  Prone to dimerization

pLV-Azurite - Mammalian Lentiviral expression

pB18cmBFPAzurite - Bacterial Expression

mAzurite 383 450 14 5  Monomer (A206K)

mAzurite-N1 - Mammalian Expression

mAzurite-C1 - Mammalian Expression

EBFP2 383 448 17 4.5  Prone to dimerization

pBad-EBFP2 - Bacterial Expression

EBFP2-N1 - Mammalian Expression

EBFP2-C1 - Mammalian Expression

EBFP2-pBAD - Bacterial Expression

moxBFP 385 448 17   Monomer (A206K)

moxBFP - Mammalian Expression

mKalama1 385 456 16 5.5  Monomer (A206K)

pBad-mKalama1 - Bacterial Expression

mTagBFP2 399 456 32 2.7 12 min Prone to dimerization

mTagBFP2-pBAD - Bacterial Expression

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Cyan

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

Aquamarine 420 474 23 3.3  Prone to dimerization

pPROEX Aqua - Bacterial Expression

pAquaN1 - Mammalian Expression

ECFP 433 475 13 4.7  Prone to dimerization

pcDNA3-CFP - Mammalian Expression

pCAG-CFP - Mammalian Expression

Cerulean 433 475 27 4.7  Monomer (A206K)

Cerulean - Mammalian Expression

pCS2+8CCerulean - Zebrafish/Xenopus/C.elegans/Sea urchin

pCS2+8NCerulean - Zebrafish/Xenopus/C.elegans/Sea urchin

mCerulean N1 - Mammalian Expression

mCerulean C1 - Mammalian Expression

mCerulean3 433 475 32 3.2  Monomer (A206K)

mCerulean3-N1 - Mammalian Expression

mCerulean3-C1 - Mammalian Expression

moxCerulean3 434 474 36 3.2  Monomer (A206K)

moxCerulean3 - Mammalian Expression

SCFP3A 433 474 17 4.5  Monomer (A206K)

pSCFP3A-C1 - Mammalian Expression

mTurquoise2 434 474 28 3.1  Monomer (A206K)

pmTurquoise2-C1 - Mammalian Expression

pmTurquoise2-N1 - Mammalian Expression

CyPet 435 477 18 5  Monomer

pCEP4CyPet-MAMM - Mammalian Expression

pCyPet-C1 - Mammalian Expression

pCyPet-His - Bacterial Expression

CYPet-N1 - Mammalian Expression

CYPet-C1 - Mammalian Expression

AmCyan1 453 486 11   Tetramer

AmCyan1-N1 - Mammalian Expression

MiCy (Midoriishi-Cyan) 472 495 25 6.6  Dimer

MiCy-N1 - Mammalian Expression

MiCy-C1 - Mammalian Expression

MiCy-pBAD - Bacterial Expression

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Green

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

iLOV 447 497    Monomer

pEiLOV-N1 - Mammalian Expression

pGEX iLOV - Bacterial Expression

iLOV-N1 - Mammalian Expression

iLOV-C1 - Mammalian Expression

AcGFP1 475 505 28   Prone to dimerization

AcGFP1-N1 - Mammalian Expression

AcGFP1-C1 - Mammalian Expression

sfGFP 485 507 54   Prone to dimerization

sfGFP-N1 - Mammalian Expression

sfGFP-C1 - Mammalian Expression

sfGFP-pBAD - Bacterial Expression

moxGFP 486 510 50   Monomer (A206K)

moxGFP - Mammalian Expression

mEmerald 487 509 39 6  Monomer (A206K)

mEmerald-N1 - Mammalian Expression

mEmerald-C1 - Mammalian Expression

mEmerald-pBAD - Bacterial Expression

EGFP 488 507 34 6  Prone to dimerization

pcDNA3-EGFP - Mammalian Expression

pCAG-GFP - Mammalian Expression

pLV-eGFP - Mammalian Lentiviral Expression

Ac5-STABLE1-neo - Insect Expression

pCS2+8CeGFP - Zebrafish/Xenopus/C.elegans/Sea urchin

pCS2+8NeGFP - Zebrafish/Xenopus/C.elegans/Sea urchin

pKT0128 - Yeast Expression

EGFP-pBAD - Bacterial Expression

mEGFP 488 507 34 6  Monomer (A206K)

pmEGFP-1 - Mammalian Expression

mEGFP-N1 - Mammalian Expression

mEGFP-C1 - Mammalian Expression

mEGFP-pBAD - Bacterial Expression

mAzamiGreen 492 505 41 5.8  Monomer

mAzami Green-N1 (mAG) - Mammalian Expression

mAzami Green-C1 (mAG) - Mammalian Expression

cfSGFP2 493 517 34 5.4  Monomer (A206K)

cfSGFP2-N - Mammalian Expression (cysteine-free SGFP2)

ZsGreen 493 505 39   Tetramer

pHIV-Zsgreen - Mammalian Expression (this is a bicistronic vector rather than a fusion protein vector)

ZsGreen1-N1 - Mammalian Expression

SGFP2 495 512 30   Monomer (A206K)

pSGFP2-C1 - Mammalian Expression

Clover 505 515 84 6.1 30 min Prone to dimerization

pcDNA3-Clover - Mammalian Expression

pFA6a-link-yoClover-Kan - Yeast Expression

mClover2 505 515    Monomer (A206K)

mClover2-N1 - Mammalian Expression

mClover2-C1 - Mammalian Expression

mClover3 506 518 85 6.5  Monomer (A206K)

pNCS-mClover3 - Bacterial Expression

mNeonGreen 506 517 93 5.7 10 min Monomer

pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression

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Yellow

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

EYFP 513 527 51 6.9  Prone to dimerization

pcDNA3-YFP - Mammalian Expression

Topaz 514 527 57   Prone to dimerization

Topaz-N1 - Mammalian Expression

Topaz-C1 - Mammalian Expression

Topaz-pBAD - Bacterial Expression

mTopaz 514 527    Monomer (A206K)

mTopaz-N1 - Mammalian Expression

mTopaz-C1 - Mammalian Expression

mTopaz-pBAD - Bacterial Expression

mVenus 515 528 53 6  Monomer (A206K)

mVenus N1 - Mammalian Expression

mVenus C1 - Mammalian Expression

pKT0090 - Yeast Expression

mVenus-pBAD - Bacterial Expression

moxVenus 514 526 44   Monomer (A206K)

moxVenus - Mammalian Expression

SYFP2 515 527 69 6  Monomer (A206K)

pSYFP2-C1 - Mammalian Expression

mGold 515 530 68 5.9  Monomer

pJL-mGold - Yeast Expression

pCaggs-mGold - Mammalian Expression

mCitrine 516 529 59 5.7  Monomer (A206K)

pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression

pCS2+8CmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin

pCS2+8NmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin

pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression

mCitrine-N1 - Mammalian Expression

mCitrine-C1 - Mammalian Expression

mCitrine-pBAD - Bacterial Expression

YPet 517 530 80 5.6  Prone to dimerization

pCEP4YPet-MAMM - Mammalian Expression

pYPet-His - Bacterial Expression

YPet-N1 - Mammalian Expression

YPet-C1 - Mammalian Expression

YPet-pBAD - Bacterial Expression

ZsYellow1 529 539 8   Tetramer

ZsYellow1-N1 - Mammalian Expression

mPapaya1 530 541 36 6.8  Monomer

mPapaya1-N1 - Mammalian Expression

mPapaya1-C1 - Mammalian Expression

mCyRFP1 528 594 18 5.6  Monomer

pNCS-mCyRFP1 - Bacterial Expression

mCyRFP1-C1 - Mammalian Expression

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Orange

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

mKusabira-Orange (mKO) 548 559 31 5 4.5 hr Monomer

pQC mKorange IX - Mammalian Expression

mKusabira Orange-C1 (mKO) - Mammalian Expression

mKO-pBAD - Bacterial Expression

mOrange 548 562 49 6.5 2.5 hr Monomer

pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression

pCS2+8CmOrange - Zebrafish/Xenopus/C.elegans/Sea urchin

pCS2+8NmOrange - Zebrafish/Xenopus/C.elegans/Sea urchin

mOrange-N1 - Mammalian Expression

mOrange-C1 - Mammalian Expression

mOrange-pBAD - Bacterial Expression

mOrange2 549 565 35 6.5 4.5 hr Monomer

mOrange2-N1 - Mammalian Expression

mOrange2-C1 - Mammalian Expression

mOrange2-pBAD - Bacterial Expression

mKO2 551 565 40 5.5  Monomer

mKO2-N1 - Mammalian Expression

mKO2-C1 - Mammalian Expression

mKO2-pBAD - Bacterial Expression

TurboRFP 553 574 62 4.4  Dimer

TurboRFP-pBAD - Bacterial Expression

tdTomato 554 581 95 4.7 1 hr Tandem-dimer

pCSCMV:tdTomato - Mammalian Expression

pFA6a-link-tdTomato-SpHis5 - Yeast Expression

tdTomato-N1 - Mammalian Expression

tdTomato-C1 - Mammalian Expression

tdTomato-pBAD - Bacterial Expression

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Red

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

mScarlet-H 551 592 15 4.8 4.4 hr Monomer

pmScarlet-H_C1 - Mammalian Expression

mNectarine 558 578 26 6.9 30 min Monomer

pBAD-mNectarine - Bacterial Expression

mRuby2 559 600 43 5.3 150 min Prone to dimerization

pcDNA3-mRuby2 - Mammalian Expression

pFA6a-link-yomRuby2-SpHis5 - Yeast Expression

mRuby2-N1 - Mammalian Expression

mRuby2-C1 - Mammalian Expression

eqFP611 559 611 35   Tetramer

eqFP611-N1 - Mammalian Expression

DsRed2 563 582 24   Tetramer

pCAG-DsRed2 - Mammalian Expression

DsRed2-N1 - Mammalian Expression

DsRed2-C1 - Mammalian Expression

DsRed2-pBAD - Bacterial Expression

mApple 568 592 37 6.5 30 min Monomer

mApple-N1 - Mammalian Expression

mApple-C1 - Mammalian Expression

mApple-pBAD - Bacterial Expression

mScarlet 569 594 71 5.3 2.9 hr Monomer

pmScarlet_C1 - Mammalian Expression

pCS2+mScarlet-C Cloning Vector - Mammalian Expression

mScarlet-I 569 593 57 5.4 0.6 hr Monomer

pmScarlet-i_C1 - Mammalian Expression

mStrawberry 574 596 26 4.5 50 min Monomer

mStrawberry-N1 - Mammalian Expression

FusionRed 580 608 18   Monomer

FusionRed-pBAD - Bacterial Expression

mRFP1 584 607 13 4.5 1 hr Monomer

pcDNA3-mRFP - Mammalian Expression

pMXs-mRFP1 - Mammalian Retroviral Expression

mCherry 587 610 16 4.5 15 min Monomer

pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression

pLV-mCherry - Mammalian Lentiviral Expression

pCS2+8CmCherry - Zebrafish/Xenopus/C.elegans/Sea urchin

pCS2+8NmCherry - Zebrafish/Xenopus/C.elegans/Sea urchin

pET mCherry LIC cloning vector (u-mCherry) - Bacterial Expression

pFA6a-link-yomCherry-SpHis5 - Yeast Expression

mCherry2 587 610    Monomer

mCherry2-N1 - Mammalian Expression

mCherry2-C1 - Mammalian Expression

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Far-Red

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

mCrimson3 588 615 -   Monomer

mCrimson3-N1 - Mammalian Expression

mCrimson3-C1 - Mammalian Expression

HcRed1 588 618 0.3   Dimer

pCAG-HcRed - Mammalian Expression

HcRed1-N1 - Mammalian Expression

HcRed1-C1 - Mammalian Expression

dKatushka 588 635 22 5.5  Dimer

dKatushka-pBAD - Bacterial Expression

mKate1.3 588 635 25   Monomer

mKate1.31-pBAD - Bacterial Expression

mPlum 590 649 4 4.5 100 min Monomer

pQC mPlum IX - Mammalian Expression

mPlum-N1 - Mammalian Expression

mPlum-C1 - Mammalian Expression

mPlum-pBAD - Bacterial Expression

mRaspberry 598 625 13  55 min Monomer

mRaspberry-N1 - Mammalian Expression

TagRFP675 598 675 4 5  Prone to dimerization

pTagRFP675-N1 - Mammalian Expression

pBAD/HisD-TagRFP675 - Bacterial Expression

mNeptune 600 650 13 5.4 35 min Prone to dimerization

pcDNA3-mNeptune2-N - Mammalian Expression

mNeptune2 600 650 21   Prone to dimerization

mNeptune2-N1 - Mammalian Expression

mNeptune2-C1 - Mammalian Expression

mCarmine 603 675 5.81 5.58  Monomer

mCarmine in pcDNA3 - Mammalian Expression

mCarmine in pRSETB - Bacterial Expression

mCardinal 604 659 17 5.3 27 min Prone to dimerization

mCardinal-N1 - Mammalian Expression

mCardinal-C1 - Mammalian Expression

mCardinal-pBAD - Bacterial Expression

mMaroon 610 652 -   Monomer

mMaroon-N1 - Mammalian Expression

mMaroon-C1 - Mammalian Expression

TagRFP657 611 657 0.34 5.3 27 min Prone to dimerization

pTagRFP657-N1 - Mammalian Expression

pTagRFP657-C1 - Mammalian Expression

pFA6a-link-yoTagRFP657-Kan - Yeast Expression

smURFP + BV 642 670 32  39 min Prone to dimerization

smURFP + BV - Mammalian Expression

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Near-Infrared

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

miRFP670 642 670 12 4.5  Monomer

pmiRFP670-N1 - Mammalian Expression

pBAD/His-miRFP670 - Bacterial Expression

iRFP670 643 670 13 4 ~5 hr Dimer

piRFP670-N1 - Mammalian Expression

pBAD/HisB-iRFP670 - Bacterial Expression

iRFP682 663 682 10 4.5 ~5hr Dimer

piRFP682-N1 - Mammalian Expression

pBAD/HisB-iRFP682 - Bacterial Expression

miRFP703 673 703 8 4.5  Monomer

pmiRFP703-N1 - Mammalian Expression

pBAD/His-miRFP703 - Bacterial Expression

iRFP702 673 702 8 4.5 ~5hr Dimer

piRFP702-N1 - Mammalian Expression

pBAD/HisB-iRFP702 - Bacterial Expression

miRFP709 683 709 4 4.5  Monomer

pmiRFP709-N1 - Mammalian Expression

pBAD/His-miRFP709 - Bacterial Expression

mIFP 683 704 7 3.5  Monomer

mIFP-N1 - Mammalian Expression

mIFP24-C1 - Mammalian Expression

IFP2.0 684 708 7   Prone to dimerization

IFP2.0-N1 - Mammalian Expression

IFP2.0-C1 - Mammalian Expression

iRFP (aka iRFP713) 690 713 6 4.5 2.8 hr Dimer

piRFP - Mammalian Expression

pBAD/His-B-iRFP - Bacterial Expression

iSplit 690 713 5   Dimer

pPAS-E - Mammalian Expression

pK-GAFm - Mammalian Expression

pWA PAS-E BAD K-GAFm - Bacterial Expression

iRFP720 702 720 6 4.5 ~5hr Dimer

piRFP720-N1 - Mammalian Expression

pBAD/HisB-iRFP720 - Bacterial Expression

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Long Stokes Shift

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

T-sapphire 399 511 26 4.9 1.3 hr Prone to dimerization

pFA6a-link-yETSapphire-CaUra3 - Yeast Expression

mT-sapphire 399 511    Monomer (A206K)

mT-Sapphire-N1 - Mammalian Expression

mT-Sapphire-C1 - Mammalian Expression

mT-Sapphire-pBAD - Bacterial Expression

mAmetrine 406 526 26 6 0.8 hr Monomer (A206K)

pBad-mAmetrine - Bacterial Expression

mAmetrine-N1 - Mammalian Expression

mAmetrine-C1 - Mammalian Expression

LSSmOrange 437 572 23 5.7 2.3 hr Monomer

pLSSmOrange-C1 - Mammalian Expression

pLSSmOrange-N1 - Mammalian Expression

pBAD-LSSmOrange - Bacterial Expression

mKeima Red 440 620 3 6.5 4.4 hr Monomer

pFA6a-link-yomKeima-CaURA3 - Yeast Expression

mKeima-Red-N1 - Mammalian Expression

mKeima-Red-C1 - Mammalian Expression

dKeima Red 440 616 22 6.5  Dimer

dKeima-Red-N1 - Mammalian Expression

LSSmKate1 463 624 3 3.2 1.7 hr Monomer

pLSSmKate1-N1 - Mammalian Expression

pLSSmKate1-C1 - Mammalian Expression

pBAD/HisD-LSSmKate1 - Bacterial Expression

LSSmKate2 460 605 4 2.7 2.5 hr Monomer

pLSSmKate2-N1 - Mammalian Expression

pBAD/HisD-LSSmKate2 - Bacterial Expression

LSSmKate2-C1 - Mammalian Expression

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Photoactivatable (e.g. off to on)

Excitation and Emission wavelengths after activation.</br>Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

Phamret 458 517 14   Monomer

Phamret-N1 - Mammalian Expression

Phamret-C1 - Mammalian Expression

PA-sfGFP 485 511    Prone to dimerization

PA-sfGFP-N1 - Mammalian Expression

PA-sfGFP-C1 - Mammalian Expression

mPA-Emerald 487 509 -   Monomer (A206K)

mPA-Emerald-N1 - Mammalian Expression

mPA-Emerald-C1 - Mammalian Expression

PA-GFP 504 517 14   Monomer (A206K)

pPAGFP-N1 - Mammalian Expression

pPAGFP-C1 - Mammalian Expression

pRSETA-PAGFP - Bacterial Expression

tandem PA-GFP-KanR - Yeast Expression

pFA6a-PA-GFP-kanMX6 - Yeast Expression

pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression

mPA-GFP-N1 - Mammalian Expression

mPA-GFP-C1 - Mammalian Expression

PATagRFP 562 595 25 5.3 75 min Prone to dimerization

pBAD/HisD-PATagRFP - Bacterial Expression

PAmCherry1 564 595 8 6.3 23 min Monomer

pPAmCherry1-N1 - Mammalian Expression

pPAmCherry1-C1 - Mammalian Expression

pBAD/HisB-PAmCherry1 - Bacterial Expression

PAmCherry2 570 596 13 6.2 18 min Monomer

pPAmCherry2-C1 - Mammalian Expression

PAmCherry3 570 596 5 6.2 25 min Monomer

pPAmCherry3-C1 - Mammalian Expression

PAmKate 568 628 5 5.6 19 min Monomer

pPAmKate-N1 - Mammalian Expression

pBAD/HisB-PAmKate - Bacterial Expression

PAiRFP1 690 717 3   Dimer

pPAiRFP1-N1 - Mammalian Expression

pBAD/HisB-PAiRFP1 - Bacterial Expression

PAiRFP2 692 719 3   Dimer

pPAiRFP2-N1 - Mammalian Expression

pBAD/HisB-PAiRFP2 - Bacterial Expression

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Photoconvertible (e.g. green to red)

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

Dendra2 490 / 553 507 / 573 23/19 6.6/6.9  Monomer

pEG545 - Worm Expression

mEos2 506 / 571 516 / 581 47 / 30 5.6/6.4  Prone to oligomerization

pRSETa mEos2 - Bacterial Expression

pFA6a-link-yomEos2-CaURA3 - Yeast Expression

mEos3.2 507 / 572 516 / 580 53 / 18 5.4/5.8 20 min Monomer

mEos3.2-N1 - Mammalian Expression

mEos3.2-C1 - Mammalian Expression

mEos4a 505 / 569 516 / 581    Prone to oligomerization

mEos4a-N1 - Mammalian Expression

mEos4a-C1 - Mammalian Expression

mEos4b 505 / 569 516 / 581    Monomer

mEos4b-N1 - Mammalian Expression

mEos4b-C1 - Mammalian Expression

dEos 505 / 569 516 / 581 55 / 20   Dimer

dEos-N1 - Mammalian Expression

tdEos 505 / 569 516 / 581 55 / 20   Tandem dimer

tdEos-N1 - Mammalian Expression

tdEos-C1 - Mammalian Expression

mKikGR 507 / 583 517 / 593 34 / 18 6.6/5.2  Monomer

mKikGR-N1 - Mammalian Expression

mKikGR-C1 - Mammalian Expression

Kaede 508 / 572 518 / 580 87 / 20 5.6/5.6  Tetramer

Kaede-N1 - Mammalian Expression

Kaede-C2 - Mammalian Expression

PSmOrange 548 / 634 565 / 662 58 / 9 6.2/5.6 1.6 hr Monomer

pPSmOrange-N1 - Mammalian Expression

pPSmOrange-C1 - Mammalian Expression

pFA6a-link-yoPSmOrange-SpHis5 - Yeast Expression

PSmOrange2 546 / 619 561 / 651 31 / 7 6.6/5.4 3.5 hr Monomer

pPSmOrange2-N1 - Mammalian Expression

pBAD-PSmOrange2 - Bacterial Expression

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Photoswitchable (e.g. off to on to off)

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

rsTagRFP 440 / 567 585 / 585 0.02 / 4.0 6.6 43 min Prone to dimerization

pBAD/HisD-rsTagRFP - Bacterial Expression

rsEmerald 491 508    

rsEmerald-pBAD - Bacterial Expression

rsGFP1 493 510    

rsGFP1-pBAD - Bacterial Expression

Dronpa3 503 518 19   Monomer

Dronpa3-N1 - Mammalian Expression

Dronpa3-C1 - Mammalian Expression

mGeos-M 503 / 569 514 / 581 44 4.7  Prone to dimerization

mGeos-M-N1 - Mammalian Expression

mGeos-M-C1 - Mammalian Expression

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Fluorescent Timers

Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids

Fast-FT 403(blue), 583(red) 466(blue), 606(red) 14.9(blue), 6.8(red) 2.8/4.1 7.1 hr (red) Monomer

pFast-FT-N1 - Mammalian Expression

pTRE-Fast-FT - Mammalian Expression

pBAD/HisB-Fast-FT - Bacterial Expression

Medium-FT 401(blue), 579(red) 464(blue), 600(red) 18.4(blue), 5.8(red) 2.7/4.7 3.9 hr (red) Monomer

pMedium-FT-N1 - Mammalian Expression

pTRE-Medium-FT - Mammalian Expression

pBAD/HisB-Medium-FT - Bacterial Expression

Slow-FT 402(blue), 583(red) 465(blue), 604(red) 11.7(blue), 4.2(red) 2.6/4.6 28 hr (red) Monomer

pSlow-FT-N1 - Mammalian Expression

pTRE-Slow-FT - Mammalian Expression

pBAD/HisB-Slow-FT - Bacterial Expression

Jump to Top</br>Empty Backbones for Fluorescent Protein Fusions (Organized by Expression Species)

Organism/Expression System Plasmids

Mammalian

Golenbock Lab Plasmids - Includes tagging with CFP, YFP, and mRFP

Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression

Verkhusha Lab Plasmids - Enhanced colors, including PSmOrange, iRFP

Gradia Lab Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean

Davidson Lab Plasmids - Includes many N- and C-terminal fluorescent proteins

Insect

Sutherland Lab Plasmids - For multicistronic expression of GFP and/or mCherry, with neomycin cassette

Zebrafish, Sea urchin, Xenopus, and C. elegans

Hamdoun Lab Plasmids - Set includes tagging with mCherry, Cerulean, Citrine, and eGFP

pPD95_75 - C-terminal GFP for C. elegans expression

pHT101-mCherry - N- or C-terminal mCherry for C. elegans expression

Yeast

Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically optimized for yeast

Lindquist Lab Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean

Bacteria

Gradia Lab Bacterial Vectors - Includes tagging with mCherry, mOrange, mCerulean

pET GFP - C-terminal GFP for bacterial expression

Davidson Lab Plasmids - Includes many fluorescent proteins in the pBAD vector

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References:

Cranfill et al.: Nature Methods July 2016, Vol. 13(7):557-62

Olympus Confocal: The Fluorescent Protein Color Palette

NIC Wiki: Nikon Imaging Center at UCSF / QB3 Wiki

FPbase: A database created by Talley Lambert at Harvard Medical School

Zeiss Microscopy: Optical Highlighters

NIC Wiki PSFP: Nikon Imaging Center at UCSF / QB3 Wiki

Stepanenko et al.: BioTechniques, Vol. 51, No. 5, November 2011, pp. 313–327

Scholarpedia: Entry by Robert Campbell

Spectra Database: Graphing site hosted at the University of Arizona

Shaner et al.: Journal of Cell Science, Vol. 120, December 2007, pp. 4247-4260

Shaner et al.: Nature Methods, Vol.5 No. 6, June 2008, pp. 545-51

Shcherbakova et al.: Journal of the American Chemical Society, May 2012, Vol. 134 No.18, pp. 7913–7923

Hoi et al.: Chemistry & Biology, October 2013, Vol. 20 No. 10, pp.1296-304

Kogure et al.: Nature Biotechnology, May 2006, Vol. 24 No. 5, pp.577-81

Shcherbo et al.: Nature Methods, September 2007, Vol. 4 No. 9, pp. 741-6

Shemiakina et al.: Nature Communications, November 2012, Vol. 3 No. 1204

Wiedenmann et al.: Journal of Biomedical Optics, Feburary 2005, Vol. 10 No. 1, p. 14003

Chu et al.: Nature Methods, May 2014 Vol. 11 No. 5, pp.572-8

Yu et al.: Nature Communications, May 2014 Vol. 15 No. 5, p. 3626<br/></br>Supplier来源:BioVector NTCC Inc.<br/>TEL电话:+86-010-53513060<br/>Website网址: http://www.biovector.net


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