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Fluorescent Proteins: Biosensors plasmids vectors生物传感器质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

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Fluorescent Proteins: Biosensors plasmids vectors生物传感器质粒载体<br/></br>Fluorescent Proteins: Biosensors

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Background

Genetically encoded fluorescent biosensors are fluorescent proteins attached to an additional protein sequence that make it sensitive to small biomolecules or other physiological intracellular processes. Fluorescent biosensors are introduced into cells, tissues or organisms to allow for detection by fluorescence microscopy as a difference in FRET efficiency, translocation of the fluorescent protein or modulation of the fluorescent properties of a single fluorescent protein. Many biosensors allow for long-term imaging and can be designed to specifically target cellular compartments or organelles. Another advantage of biosensors is that they permit investigation of a signaling pathway or measurement of a biomolecule while largely preserving spatial and temporal cellular processes.</br>The following plasmids encode fluorescent biosensors that were designed to measure specific small biomolecules or the activity of specific genes via circularly permuted fluorescent protein (cpFP) and/or FRET-based fluorescent protein constructs. Custom biosensors can also be constructed using the cpFRET kit from the Pertz laboratory.</br>Small Molecule Biosensors

Category Purpose Principal Investigator Plasmids

Acetylcholine Family of ACh sensors (GACh) based on G-protein-coupled receptors Yulong Li See Plasmids

Arabinose Monitor intracellular levels of arabinose Wolf Frommer See Plasmid

ATP:ADP Monitor the ATP:ADP ratio during live-cell imaging Gary Yellen See Plasmids

ATP Multicolor ATP indicators Tetsuya Kitaguchi See Plasmids

Calcium Large palette of genetically encoded Ca2+ indicators (GECOs) Robert Campbell See Plasmids

Calcium tdTomato-mAmetrine1.2 Ca2+ sensors Robert Campbell See Plasmids

Calcium Calcium indicator based on C-terminal domain of Opsanus troponin C Oliver Griesbeck See Plasmids

Calcium High-performing calcium indicator based on C-terminal domain of troponin C Oliver Griesbeck See Plasmids

Calcium Calcium indicator for neural activity imaging (GCaMP5G) Loren Looger & Douglas Kim See Plasmids

Calcium Updated calcium sensors for imaging neural activity (GCaMP6) Douglas Kim See Plasmids

Calcium Fast response calcium sensors (GCaMP3-derived) Katalin Torok See Plasmids

cAMP (cyclic AMP) Improved yellow fluorescent cAMP indicator (Flamindo2) Tetsuya Kitaguchi See Plasmids

cAMP (cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Tetsuya Kitaguchi See Plasmid

cGMP (cyclic GMP) Green fluorescent probe for cGMP Tetsuya Kitaguchi See Plasmid

Dopamine Genetically encoded GPCR-activation-based-DA (GRABDA) sensors Yulong Li See Plasmids

Glucose Monitor glucose levels and transport Wolf Frommer See Plasmids

Glucose Green fluorescent glucose indicators for living cells (Glifons) Tetsuya Kitaguchi See Plasmids

Glutamate Visualize glutamate neurotransmission Loren Looger See Plasmids

Glutamate Monitor glutamate transport and metabolism Wolf Frommer See Plasmids

Glutamate Ultrafast glutamate sensors Katalin Torok See Plasmids

Hydrogen Peroxide (H2O2) Measure changes in intracellular hydrogen peroxide concentration Vsevolod Belousov See Plasmids

Lactate Measure lactate concentration and lactate fluxes Luis Felipe Barros See Plasmids

Magnesium Fluorescent Mg2+ sensor Maarten Merkx See Plasmids

Maltose Monitor maltose levels and metabolism Wolf Frommer For Bacteria

For Plants

NADH-NAD+ Fluorescent biosensor of the cytosolic NADH-NAD+ redox state Vsevolod Belousov See Plasmids

NADH-NAD+ Fluorescent biosensor of the cytosolic NADH-NAD+ redox state Gary Yellen See Plasmids

Norepinephrine Family of GPCR activation-based NE (GRABNE) sensors Yulong Li See Plasmids

pH Image intracellular pH in live cells Gary Yellen See Plasmids

pH pH biosensor to detect concentrative nucleoside transport Robert Campbell See Plasmids

pH Fluorescent pH biosensor Vsevolod Belousov See Plasmids

pH Mitochondrial pH biosensor Nicolas Demaurex See Plasmids

Phosphate Monitor inorganic phosphate transport and metabolism Wolf Frommer See Plasmids

PIP3 & H2O2 Dual PIP3 and hydrogen peroxide biosensor Vsevolod Belousov See Plasmid

redox/ROS Redox-sensitive cytosolic protein sensor Ulrich Hammerling See Plasmids

Ribose Monitor ribose uptake and metabolism Wolf Frommer See Plasmids

Sucrose Monitor sucrose levels and flux Wolf Frommer See Plasmids

Tryptophan Visualize intracellular tryptophan levels and flux Wolf Frommer See Plasmids

Voltage Fast fluorescent protein voltage sensor Thomas Knopfel See Plasmids

Voltage Detection of single action / excitatory potentials in individual neurons and dendrites Vincent Pieribone See Plasmids

Voltage Ultrafast fluorescent voltage sensor for detection of single action potentials Michael Lin See Plasmids

Zinc Visualize free zinc levels in live cells Maarten Merkx See Plasmids

Zinc Robust red zinc sensors Maarten Merkx See PlasmidsBack to Top

Gene-specific Biosensors

Gene Purpose Principal Investigator Plasmids

Actin Quantify actin polymerization with mEGFP-actin and sREACh-actin monomers Ryohei Yasuda See Plasmids

Arf6 Visualize Arf6 activation in live cells Martin Schwartz See Plasmids

Aurora B Sensor for Aurora B substrate phosphorylation Daniel Gerlich See Plasmids

CaMKIIa Measure CaMKII activation in dendritic spines Ryohei Yasuda See Plasmids

Caspase 3, 8/10 Reporter proteins for caspase activity Peter Sorger See Plasmids

Caspase 3 Reporter protein for caspase 3 activity Robert Campbell See Plasmid

Caspase 3 Reporter protein for caspase 3 activity Vladislav Verkhusha See Plasmid

Cdt NIR reporter for cell cycle progression Vladislav Verkhusha See Plasmid

CyclinB-Cdk1 CyclinB1-Cdk1 kinase activity Jonathon Pines See Plasmids

GAT1 Detect assembly and oligomerization of GABA transporter (GAT1) Henry Lester See Plasmid

Geminin NIR reporter for cell cycle progression Vladislav Verkhusha See Plasmid

IκBα NIR reporter for canonical activation of NF-κB Vladislav Verkhusha See Plasmid

Ras GTPase Sensors for the activation of Ras GTPase with slow (FRas) and fast (FRas-F) kinetics Karel Svoboda See Plasmids

Rho GTPases Biosensors for characterizing dynamics of Rho family GTPases in living cells Klaus Hahn See Plasmids

RhoA Visualize RhoA activity during cell migration Klaus Hahn See Plasmids<br/></br>Supplier来源:BioVector NTCC Inc.<br/>TEL电话:+86-010-53513060<br/>Website网址: http://www.biovector.net


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