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pMADΩphr63::spec BioVector NTCC质粒载体菌株细胞基因保藏中心

  • 价  格:¥79835
  • 货  号:pMADΩphr63::spec
  • 产  地:北京
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Plasmid Name名称:pMADΩphr63::spec
Description描述:The 5′ and 3′ regions of phr63 genes were amplified using primer sets 7557Amont2-F/7557Amont2-R and 7557Aval-F/Aval7557-R, respectively, with B. thuringiensis HD73 genomic DNA as the template. The 5′ end was purified as an NcoI/KpnI fragment and the 3′ end as an XbaI/EcoRI fragment. The spectinomycin resistance cassette was purified as a KpnI/XbaI fragment from pUC18Ωspec and was inserted together with the 5′ and 3′ regions of phr63 between the NcoI and EcoRI sites of pMAD.
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