首页 » pNV118.0 pNV119.0 plasmid vector诺卡氏菌穿梭表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pNV118.0 pNV119.0 plasmid vector诺卡氏菌穿梭表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥59865
  • 货  号:pNV118.0 pNV119.0
  • 产  地:北京
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pNV118.0 pNV119.0 plasmid vector诺卡氏菌穿梭表达载体质粒

Nocardia-E. coli shuttle plasmid vectors
pNV18 and pNV19
are constructed by inserting a 1.8 kb DNA fragment carrying the pAL5000 origin of replication (Snapper et al., 1990; Stolt & Stoker, 1997) into the unique NheI site of pK18 or pK19 (Pridmore, 1987). These vectors have the following features:
Small in size.
Multiple cloning site. In the latest version, HindIII, SphI, PstI, SalI, HincII, XbaI, BamHI, KpnI, and EcoRI sites are available.
The lacZ gene (α fragment) enables blue-white selection in E. coli.
The aph gene derived from Tn5 (Beck et al., 1982) confers kanamycin and neomycin resistance not only to E. coli but also to Nocardia spp.
Broad host-range: N. farcinica, N. asteroides, to say nothing of mycobacteria.

pNV18.1 pNV19.1 Version "1" vectors are available. EcoRI and SalI (HincII) sites located at the pAL5000 origin of replication were removed from the version "0" vectors by site-directed mutatagenesis (May, 2006), details of which has been reported in Jpn. J. Infect. Dis. 60:45-47 (2007)

Nucleotide sequence Nucleotide sequence
Version 0 informations are here: [pNV18.0|pNV19.0].
pNV118 and pNV119
are high-copy-number versions of pNV18.1 or pNV19.1, respectively. Difference between new versions and their parents is only 3-bp (Bourn et al., 2007).
pNV118.0 pNV119.0 Version "0" vectors are available. You can get more information from nBlog.

Nucleotide sequence Nucleotide sequence
pNV28 and pNV128
are hygromycin-resistant version of pNV18.1 or pNV118.0, repectively. The ORF of aph gene was replaced with that of hph gene (Zalacain et al., 1986) by the Red-ET technology, meaning the hph gene is drived by the aph-promoter.

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