- 病原微生物-细菌共 14164 条
NR-49806 Escherichia coli K-12, Strain IM08B金葡菌限制修饰系统改造型大肠杆菌菌株BioVector NTCC典型培养物保藏中心
NR-49806??Escherichia coli, K-12, Strain IM08B(Bacteria)|Escherichia coli|K-12, Strain IM08B|-60°C or colder|I Monk, T Stinear, T Foster Escherichia coli (E. coli) K-12, strain IM08B is an insertion mutant produced in E. coli K-12, strain DC10B via recombination-mediated genetic engineering (recombineering), and contains the hsdM (methylase) and hsdS (specificity) genes from Staphylococcus aureus (S. aureus), strain NRS384 clonal complex 8 (CC8). E. coli K-12, strain DC10B is a universal host for constructing plasmids for introduction into staphylococci and was deposited as Δdcm. The lack of methylation allows plasmid DNA to bypass a conserved type IV restriction-modification (RM) barrier in staphylococci which has been identified as a major barrier to transformation with foreign DNA. E. coli K-12, strain IM08B was deposited as resistant to streptomycin. Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth containing 25 ?g per mL streptomycin supplemented with 10% glycerol. 大肠杆菌(大肠杆菌) K-12,菌株IM08b是在 e中产生的插入突变体. COLI K-12,菌株DC10B通过重组介导的基因工程(重组化),并含有 HSDM (甲基酶)和 HSDS (特异性)基因来自金黄色葡萄球菌(金黄色葡萄球菌),菌株NRS384克隆复合物8(CC8). e. COLI K-12,菌株DC10B是用于构建用于引入葡萄球菌的质粒的通用宿主,并沉积为δ2DCM/ i>.缺乏甲基化允许质粒DNA在葡萄球菌中绕过保守的IV型限制性改性(RM)屏障,该葡萄球菌被鉴定为用外来DNA转化的主要屏障. e. Coli K-12,菌株IM08b被沉积为耐链霉素. 每个小瓶在含有10%甘油补充有10%甘油的胰蛋白酶肉汤中含有大约0.5ml的细菌培养物中的细菌培养物.
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