细菌CRISPR/Cas9载体质粒列表-BioVector NTCC保藏中心2014 updated

细菌CRISPR/Cas9载体质粒列表-BioVector NTCC保藏中心2014 updated

 Biovector

pET-28b-Cas9-His

pET-28b-Cas9-His

 Biovector

pwtCas9-bacteria

pwtCas9-bacteria

 Biovector

pET28a/Cas9-Cys

pET28a/Cas9-Cys

 Biovector

pCas 大肠杆菌基因编辑质粒RED重组Cas9载体-BioVector NTCC Inc.

pCas Vector backbonepKD46 (Search Vector Database) Backbone size w/o insert (bp)6300 Modifications to backboneReplace Amp with Kan Vector typeBacterial Expression, CRISPR ; Lambda Red recombinase GROWTH IN BACTERIA Bacterial Resistance(s)Kanamycin Growth Temperature30°C Growth Strain(s)DH5alpha Growth instructionsMust be grown at 30C! Vector has temperature-sensitive replication (RepA101ts) Copy numberLow Copy GENE/INSERT Gene/Insert namecas9 SpeciesS. pyogenes MGAS5005 Insert Size (bp)4500 Promoternative cas9 promoter

 Biovector

pET-Cas9-6xHis

pET-Cas9-6xHis

 Biovector

pET-NLS-Cas9-6xHis

pET-NLS-Cas9-6xHis

 Biovector

SP-Cas9

SP-Cas9

 Biovector

pdCas9

pdCas9

 Biovector

pMJ841

pMJ841

 Biovector