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载体名称：pCambia1380植物表达载体
基本信息
| 出品公司: | Cambia |
|---|---|
| 载体名称: | pcambia1380, pCambia1380植物表达载体 |
| 质粒类型: | 植物表达载体 |
| Genbank序列号: | AF234301 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8860 bp |
| 5 测序引物及序列: | -- |
| 3 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | Kanamycin |
| 筛选标记: | HptII/ hygromycin B |
| 备注: | 本载体用于感染植物细胞，研究外源基因在植物组织细胞中的作用。在大肠杆菌中，该载体是卡那抗性，在植物细胞中，可以利用潮霉素B进行筛选。 |
| 产品目录号: | -- |
| 稳定性: | 稳定 |
| 组成型: | 非组成型 |
| 病毒/非病毒: | 非病毒 |
质粒图谱
载体序列
LOCUS pCambia1380 8860 bp DNA circular SYN 24-APR-2000 DEFINITION Binary vector pCAMBIA-1380, complete sequence. ACCESSION AF234301 VERSION AF234301.1 GI:7638088 KEYWORDS . SOURCE Binary vector pCAMBIA-1380 ORGANISM Binary vector pCAMBIA-1380 other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biovector.com/ COMMENT VNTAUTHORNAME|biovector.com| REFERENCE 1 (sites) AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P. TITLE The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994) PUBMED 7919218 REFERENCE 2 (bases 1 to 8860) AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W., Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L., Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z., Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A. TITLE A comprehensive set of modular vectors for advanced manipulations and efficient transformation of plants JOURNAL Unpublished REMARK Full description of constructs REFERENCE 3 (bases 1 to 8860) AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W., Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L., Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z., Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A. TITLE Direct Submission JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / GPO Box 3200, Canberra, ACT 2601, Australia FEATURES Location/Qualifiers source 1..8860 /organism="Binary vector pCAMBIA-1380" /mol_type="other DNA" /db_xref="taxon:118393" misc_feature 61..313 /note="nos (nopaline synthase) 3UTR (polyA signal)" misc_feature 351..376 /note="right border T-DNA repeat" misc_feature complement(1417..2417) /note="STA region from pVS1 plasmid" rep_origin complement(3010..4010) /note="pVS1-REP; replication origin from pVS1" misc_feature complement(4420..4680) /note="bom site from pBR322" rep_origin complement(4820..5100) /note="pBR322 origin of replication" CDS complement(5391..6185) /note="aadA (kanamycin resistance) gene amplified from pIG121Hm" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAF65355.1" /db_xref="GI:7638089" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 6610..6635 /note="left border repeat from C58 T-DNA" misc_feature 6702..6910 /note="CaMV 3UTR (polyA signal)" CDS complement(6926..7951) /note="hptII (hygromycin resistance) gene" /codon_start=1 /product="hygromycin phosphotransferase" /protein_id="AAF65356.1" /db_xref="GI:7638090" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKK" promoter complement(7987..8773) /note="CaMV35S2; CaMV 35S promoter, duplicated" misc_feature complement(8785..8821) /note="pUC8 MCS; polylinker" ORIGIN 1 ctagccacca ccaccaccac cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat 61 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg 121 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg 181 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg 241 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg 301 ttactagatc gggaattaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa 361 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt 421 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat 481 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga 541 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg 601 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag 661 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac 721 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct 781 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga 841 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac 901 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct 961 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc 1021 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc 1081 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc 1141 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct 1201 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac 1261 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc 1321 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac 1381 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc 1441 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat 1501 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta 1561 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg 1621 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc 1681 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg 1741 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg 1801 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg 1861 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg 1921 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc 1981 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca 2041 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg 2101 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca 2161 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac 2221 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca 2281 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg 2341 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat 2401 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt 2461 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta 2521 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa 2581 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt 2641 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc 2701 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg 2761 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag 2821 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc 2881 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa 2941 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg 3001 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac 3061 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg 3121 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg 3181 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag 3241 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg 3301 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg 3361 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg 3421 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc 3481 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc 3541 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc 3601 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg 3661 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag 3721 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt 3781 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa 3841 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg 3901 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact 3961 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact 4021 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa 4081 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc 4141 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga 4201 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg 4261 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 4321 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 4381 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 4441 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 4501 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 4561 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 4621 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 4681 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 4741 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 4801 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 4861 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 4921 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 4981 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 5041 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 5101 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 5161 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 5221 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 5281 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 5341 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat 5401 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca 5461 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag 5521 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact 5581 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc 5641 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg 5701 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat 5761 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga 5821 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca 5881 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca 5941 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg 6001 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa 6061 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta 6121 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta 6181 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt 6241 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa 6301 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt 6361 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc 6421 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat 6481 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac 6541 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg 6601 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa 6661 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat 6721 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa 6781 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac 6841 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga 6901 tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc 6961 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc 7021 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac 7081 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa 7141 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc 7201 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt 7261 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta 7321 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga 7381 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac 7441 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata 7501 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc 7561 tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa 7621 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga 7681 tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg 7741 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat 7801 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc 7861 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga 7921 cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga tctgcgaaag 7981 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga 8041 aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc 8101 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt 8161 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg 8221 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct 8281 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc 8341 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt 8401 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt 8461 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc 8521 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat 8581 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag 8641 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag 8701 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct 8761 ccaccatgtt gggcccggcg cgccgaattc ccggggatcc gtcgacctgc agccaagctt 8821 taagaggagt ccaccatggt agatctgact agtgttaacg
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