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载体名称：pCambia2200植物表达载体
基本信息
| 出品公司: | Cambia |
|---|---|
| 载体名称: | pCambia2200 |
| 质粒类型: | 植物表达载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | CAMV35S |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8895 bp |
| 5 测序引物及序列: | -- |
| 3 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氯霉素 |
| 筛选标记: | NPTII |
| 备注: | -- |
| 产品目录号: | 2200 |
| 稳定性: | 稳定表达 |
| 组成型: | 非组成型 |
| 病毒/非病毒: | 非病毒 |
质粒图谱
载体序列
LOCUS pCambia2200 8895 bp ds-DNA circular SYN 16-四月-2013 DEFINITION Agrobacterium binary vector for plant transformation, with kanamycin- and chloramphenicol-resistance genes. ACCESSION AF234313 VERSION . KEYWORDS pCAMBIA2200 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT This file is created by Vector NTI COMMENT The GenBank record was corrected by inserting a G at position 2281. FEATURES Location/Qualifiers source 1..8895 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" CDS 1219..1848 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label="pVS1 StaA" CDS 2277..3350 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label="pVS1 RepA" /label=" " /protein_id=" " misc_feature 3954..4094 /label="bom" /label="basis of mobility region from pBR322" rep_origin complement(4280..4868) /direction=LEFT /label="ori" /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5139..5798) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label="CmR" /label="confers resistance to chloramphenicol" misc_feature 6326..6350 /label="LB T-DNA repeat" /label="left border repeat from nopaline C58 T-DNA" polyA_signal 6428..6602 /label="CaMV poly(A) signal" /label="cauliflower mosaic virus polyadenylation signal" CDS complement(6659..7456) /codon_start=1 /gene="aph(3)-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label="NeoR/KanR" /label="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" promoter complement(7519..8196) /label="CaMV 35S promoter (enhanced)" /label="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" promoter 8423..8453 /label="lac promoter" /label="promoter for the E. coli lac operon" protein_bind 8461..8477 /bound_moiety="lac repressor encoded by lacI" /label="lac operator" /label="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 8485..8501 /label="M13 rev" /label="common sequencing primer, one of multiple similar variants" CDS 8497..8730 /codon_start=1 /gene="lacZ (truncated)" /product="LacZ-alpha fragment of?beta-galactosidase" /label="lacZ-alpha" /protein_id=" " misc_feature 8511..8567 /gene=" " /label="MCS" /label="pUC18/19 multiple cloning site" primer_bind complement(8571..8587) /label="M13 fwd" /label="common sequencing primer, one of multiple similar variants" misc_feature 8790..8814 /label="RB T-DNA repeat" /label="right border repeat from nopaline C58 T-DNA" ORIGIN 1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct 61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca 121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt 181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca 241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga 301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca 361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg 421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca 481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg 541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg 601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg 661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga 721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga 781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg 841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac 901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac 961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt 1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg 1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt 1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca 1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc 1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg 1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa 1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc 1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg 1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc 1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa 1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag 1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac 1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc 1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta 1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca 1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc 1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca 2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa 2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc 2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc 2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga 2281 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg 2341 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg 2401 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag 2461 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc 2521 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc 2581 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc 2641 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac 2701 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca 2761 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg 2821 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg 2881 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg 2941 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg 3001 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc 3061 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc 3121 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac 3181 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt 3241 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag 3301 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc 3361 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg 3421 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga 3481 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa 3541 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg 3601 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc 3661 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct 3721 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc 3781 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt 3841 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 3901 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 3961 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 4021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 4081 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 4141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 4201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 4261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 4321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 4381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 4441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 4501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 4561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 4621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 4681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 4741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 4801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 4861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 4921 cacgttaagg gattttggtc atgcatgata tatctcccaa tttgtgtagg gcttattatg 4981 cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag caattatgtg 5041 cttagtgcat ctaatcgctt gagttaacgc cggcgaagcg gcgtcggctt gaacgaattt 5101 ctagctagag gatcgcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca 5161 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca 5221 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 5281 attgtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 5341 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa 5401 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 5461 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 5521 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg 5581 aactccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg 5641 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta 5701 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat 5761 atatcaacgg tggtatatcc agtgattttt ttctccatga tgtttaactt tgttttaggg 5821 cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg 5881 taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acatgtcata 5941 acaagaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct 6001 ggaccagttg cgtgacggca gttacgctac ttgcattaca gcttacgaac cgaacgaggc 6061 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac 6121 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg 6181 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc 6241 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc 6301 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc 6361 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa 6421 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat 6481 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc 6541 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga 6601 aactcgagct tgtcgatcga ctctagctag aggatcgatc cgaaccccag agtcccgctc 6661 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac 6721 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg 6781 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc 6841 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgtgtcacga 6901 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga 6961 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac 7021 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg 7081 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag 7141 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag 7201 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg 7261 ctgcctcgtc ctggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg 7321 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg 7381 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat 7441 cttgttcaat ccccatggtc gatcgacaga tctgcgaaag ctcgagagag atagatttgt 7501 agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga 7561 ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca 7621 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc 7681 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt 7741 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg 7801 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga 7861 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg 7921 agagtgtcgt gctccaccat gttatcacat caatccactt gctttgaaga cgtggttgga 7981 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg 8041 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca 8101 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg 8161 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact 8221 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt ggcaagctgc 8281 tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 8341 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 8401 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 8461 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcgagc 8521 tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc actggccgtc 8581 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 8641 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 8701 cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca gattgtcgtt 8761 tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga 8821 gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt ttatccgttc 8881 gtccatttgt atgtg
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