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载体名称:pCambia1201植物表达载体
基本信息
出品公司: | Cambia |
---|---|
载体名称: | pCambia1201, pCambia 1201 |
质粒类型: | 植物表达载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | 35S promoter |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 12002 bp |
5 测序引物及序列: | 35S promoter: CTATCCTTCGCAAGACCCTTC |
3 测序引物及序列: | -- |
载体标签: | GusA报告基因 |
载体抗性: | 氯霉素和潮霉素 |
筛选标记: | HPTII |
备注: | -- |
产品目录号: | 1201 |
稳定性: | 稳定表达 |
组成型: | 非组成型 |
病毒/非病毒: | 非病毒 |
质粒图谱
载体序列
LOCUS pCambia1201 12002 bp ds-DNA circular SYN 17-五月-2013 DEFINITION Agrobacterium binary vector for plant transformation, with hygromycin- and chloramphenicol-resistance and GUS genes. ACCESSION AF234293 VERSION . KEYWORDS pCAMBIA1201 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT This file is created by Vector NTI COMMENT The GenBank record was corrected by inserting a G at position 4743. FEATURES Location/Qualifiers source 1..12002 /organism="synthetic DNA construct" /lab_host="Plant Cells" /mol_type="other DNA" CDS join(2..16,207..2024) /codon_start=1 /product="beta-glucuronidase" /label="GUS" /label="This version of the gusA gene has a 5 extension with a catalase intron to ensure expression in plants but not bacteria." /protein_id=" " intron 17..206 /label="cat1 intron" /label="castor bean catalase intron, modified" CDS 2031..2048 /codon_start=1 /product="6xHis affinity tag" /label="6xHis" /translation="HHHHHH" terminator 2083..2335 /label="NOS terminator" /label="nopaline synthase terminator and poly(A) signal" misc_feature 2357..2381 /label="RB T-DNA repeat" /label="right border repeat from nopaline C58 T-DNA" CDS 3681..4310 /codon_start=1 /product="stability protein from Pseudomonas plasmid pVS1" /label="pVS1 StaA" CDS 4739..5812 /codon_start=1 /product="replication protein from Pseudomonas plasmid pVS1" /label="pVS1 RepA" /label=" " /protein_id=" " misc_feature 6416..6556 /label="bom" /label="basis of mobility region from pBR322" rep_origin complement(6742..7330) /direction=LEFT /label="ori" /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7601..8260) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label="CmR" /label="confers resistance to chloramphenicol" misc_feature 8788..8812 /label="LB T-DNA repeat" /label="left border repeat from nopaline C58 T-DNA" polyA_signal 8890..9064 /label="CaMV poly(A) signal" /label="cauliflower mosaic virus polyadenylation signal" CDS complement(9104..10129) /codon_start=1 /product="hygromycin B phosphotransferase" /label="HygR" /label="confers resistance to hygromycin" promoter complement(10197..10874) /label="CaMV 35S promoter (enhanced)" /label="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" promoter 11101..11131 /label="lac promoter" /label="promoter for the E. coli lac operon" protein_bind 11139..11155 /bound_moiety="lac repressor encoded by lacI" /label="lac operator" /label="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 11163..11179 /label="M13 rev" /label="common sequencing primer, one of multiple similar variants" misc_feature 11189..11245 /gene=" " /label="MCS" /label="pUC18/19 multiple cloning site" primer_bind complement(11249..11265) /label="M13 fwd" /label="common sequencing primer, one of multiple similar variants" promoter 11642..11987 /label="CaMV 35S promoter" /label="strong constitutive promoter from cauliflower mosaic virus" ORIGIN 1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct 61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga 121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt 181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa 241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg 301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag 361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt 421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg 481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg 541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac 601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga 661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg 721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg 781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca 841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag 901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc 961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg 1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct 1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg 1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct 1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga 1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca 1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact 1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga 1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg 1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct 1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt 1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac 1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata 1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat 1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat 1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag 1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga 1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc 2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg 2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt 2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga 2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata 2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa 2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt 2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg 2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg 2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca 2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc 2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa 2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt 2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat 2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg 2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga 2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc 3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt 3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg 3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct 3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc 3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg 3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga 3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt 3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac 3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc 3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta 3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa 3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag 3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc 3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc 3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg 3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg 3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg 4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac 4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg 4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct 4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg 4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg 4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag 4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt 4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac 4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat 4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag 4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg 4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt 4741 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt 4801 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc 4861 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc 4921 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt 4981 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt 5041 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg 5101 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt 5161 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga 5221 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc 5281 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca 5341 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg 5401 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat 5461 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt 5521 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg 5581 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga 5641 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg 5701 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct 5761 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga 5821 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt 5881 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg 5941 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa 6001 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct 6061 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct 6121 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg 6181 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc 6241 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg 6301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 6361 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 6421 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 6481 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 6541 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 6721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 6781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 6841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 6901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 6961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 7381 ctcacgttaa gggattttgg tcatgcatga tatatctccc aatttgtgta gggcttatta 7441 tgcacgctta aaaataataa aagcagactt gacctgatag tttggctgtg agcaattatg 7501 tgcttagtgc atctaatcgc ttgagttaac gccggcgaag cggcgtcggc ttgaacgaat 7561 ttctagctag aggatcgcac caataactgc cttaaaaaaa ttacgccccg ccctgccact 7621 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg 7681 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc 7741 ccattgtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg 7801 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga 7861 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc 7921 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa 7981 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac 8041 ggaactccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact 8101 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt 8161 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg 8221 atatatcaac ggtggtatat ccagtgattt ttttctccat gatgtttaac tttgttttag 8281 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg 8341 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacatgtca 8401 taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt 8461 ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga accgaacgag 8521 gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt 8581 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc 8641 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc 8701 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg 8761 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac 8821 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt 8881 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg 8941 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga 9001 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga 9061 gaaactcgag cttgtcgatc gacagatccg gtcggcatct actctatttc tttgccctcg 9121 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc 9181 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga 9241 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc 9301 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg 9361 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg 9421 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc 9481 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg 9541 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag 9601 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg 9661 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc 9721 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatagcct 9781 ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac 9841 cctgtgcacg gcgggagatg caataggtca ggctctcgct aaactcccca atgtcaagca 9901 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga 9961 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga 10021 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt 10081 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc 10141 ccccgggatc tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag 10201 cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt 10261 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag 10321 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg 10381 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt 10441 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat 10501 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt 10561 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt 10621 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc 10681 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag 10741 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt 10801 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt 10861 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt 10921 agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc 10981 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 11041 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 11101 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 11161 cacaggaaac agctatgacc atgattacga attcgagctc ggtacccggg gatcctctag 11221 agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg 11281 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 11341 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 11401 aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttagcttca 11461 tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt 11521 tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc 11581 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa 11641 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta 11701 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt 11761 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac 11821 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag 11881 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc 11941 aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactcttg 1 2001 ac
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