| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pLenti6.3/V5-DEST |
| 质粒类型: | 慢病毒表达载体；Gateway系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CMV |
| 克隆方法: | Gateway |
| 载体大小: | 9387 bp |
| 5' 测序引物及序列: | CMV-F |
| 3' 测序引物及序列: | WPRE-R |
| 载体标签: | V5 Epitope Tag |
| 载体抗性: | Ampicillin |
| 筛选标记: | Blasticidin |
| 备注: | The upgrade from pLenti6 to pLenti6.3 is in the addition of a WPRE cassette. Lentiviral Gateway® destination vector for expression of a C- terminally V5-tagged protein. |
| 产品目录号: | V533-06 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
pLenti6.3/V5-DEST慢病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格：¥1920
- 货 号：BiovectorLenti6.3/V5-DEST
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
DescriptionInvitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway® Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit (catalog K5330-00). The pLenti6.3⁄V5-DEST™ Gateway® Vector Kit contains the Gateway®-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.Advantages• Stable expression• Long-term experiments• Accurate titer of functional virus• Flexible and versatile Gateway® recombination cloning technologyKey Features• HiPerform™ WPRE and cPPT elements• CMV promoter • V5 epitope tag at C terminus• Blasticidin selection载体序列
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