RNAi慢病毒载体pLKO.1 慢病毒包装载体(invitrogen)pLP1, pLP2,pLP VSV-G, 配套菌株Stbl3
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- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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RNAi载体(Ambion),pSilencer1.0,pSilencer 2.1-U6 hygro,pSilencer 3.1-H1
RNAi载体(Ambion)pSilencer 4.1-CMV neo,pSilencer 4.1-CMV puro
Dr Guo,Senior Scientist
手机189OI268599
电邮biovector@163.com
Biovector实验室质粒载体菌株基因细胞株列表网址http://biovector.blog.163.com
逆病毒载体(addgene )pLVTH,pLNCX2,pQCXIH,pMSCVpuro
Gateway系统(invitrogen)pENTR3C, pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR
信号通路报告载体(Stratagene)p53-Luc,pAP-1-Luc, pNF-κB-Luc,pSRE-Luc,pFA2-Elk1,pFC-MEKK,pFR-luc
信号通路报告载体(Promega)pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75
哺乳动物表面展示系统(Invitrogen)pDisplay
腺病毒系统(Stratagene)pAdEasy-1,pShuttle-CMV,pShuttle
GeneSwitch System(Invitrogen)pSwitch,pGene v5-His B
LacSwith II哺乳动物诱导表达系统(Stratagene)pOPRSVI ,pOPI3CAT,pCMVLacI
蜕皮激素诱导系统(Invitrogen)pIND,pVgRxR
Cytotrap Two-Hybrid System(Stratagene)pSos, pSos MAFB, pMyr
酵母单杂交系统(Clontech)pHis2,pHISi,pLacZi,菌株YM4271
腺相关病毒系统(Stratagene)pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP,pCMV-MCS
哺乳动物表达载体:pcDNA3.1(neo),pcDNA3.1(neo),pcDNA3.1(hyg)
哺乳动物双杂交系统(Promega)pACT,pBIND,pACT-MyoD,pBIND-Id,pG5luc
哺乳动物双杂交系统(Promega)pACT,pBIND,pACT-MyoD,pBIND-Id,pG5luc
昆虫细胞表达载体(Invitrogen):pFastBac1,pFastBac1-Gus,pFastBacHT A,pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A,配套大肠杆菌DH10Bac
pMIB v5-His B,pIZT/V5-His
启动子增强子报告载体(Promega)pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter,pRL-TK,pRL-CMV,pRL-SV40
荧光蛋白报告载体(Clontech):pEGFP-C1, pEGFP-N1,pEGFP-1, pEGFP-N3 ,pDsRed1-C1, pDsRed1-N1,pIRES2-EGFP, pLEGFP-C1,pLEGFP-N1,pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pECFP-N1,pECFP-C1,pAcGFP1-1pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc,pDsRED2-Peroxi ,pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pmCherry-N1,pmCherry-C1
重组蛋白乳汁表达系统(Invitrogen):pBC1
酵母表达载体(Invitrogen):pGAPZα A,pPICZα A,pYES2,pYES6/CT,pPink-hc
配套毕赤酵母X33,KM71,KM71H,GS115,SMD1168,SMD1163,
pYES2,pYES6/CT,配套酿酒酵母INVSc1
毕赤酵母表达系统(Invitrogen)pPink-hc,配套毕赤酵母Pichiapink
慢病毒表达载体(Clontech),pLVX-DsRed-Monomer-N1, pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1
RNAi载体(clontech),RNAi-Ready pSIREN-Retro Q,RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide)
pMIR-REPORT Luciferase
RNAi慢病毒载体(addgene),pLKO.1
慢病毒包装载体(invitrogen)pLP1, pLP2,pLP VSV-G, 配套菌株Stbl3
RNAi载体(Ambion),pSilencer1.0,pSilencer 2.1-U6 hygro,pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo,pSilencer 4.1-CMV neo,
pSilencer 4.1-CMV puro
微生物类型 质粒名 特性
枯草芽孢杆菌 168
野生型枯草芽孢杆菌FQ13,FG24 , FZB42
枯草芽孢杆菌
pHY300PLK
pHCMC04
pMUTIN4 基因组整合型载体
pBesT502 带有枯草杆菌强启动子PrepU
荧光假单孢菌
FQ99422
Dzg-994-28
广宿主质粒(包括假单孢菌等多种革兰氏阴性菌)
pVLT33(putmini-Tn5公司产品) Ptac启动子下,表达型。
pME6032 Ptac强启动子下的表达型质粒
pLAFR3 Cos载体
p2444 带GFP报告基因
pRT102 带有lux荧光素报告基因
pRL1063a 带有lux荧光素报告基因
pUT-miniTn5
Tn5载体
pUT-miniTn5
pRL1063a 带有lux荧光素报告基因
pRT102 带有lux荧光素报告基因
双(三)亲本杂交受体菌
S17-1
S17л
helper
pRK2013 辅助三亲本杂交的质粒
特殊用途载体
pUC18Not 只含有一个NotI酶切位点
pGEX-6p-2 克隆载体
高丝氨酸内脂类信号分子指示系统 NTL4/pZLR4 高丝氨酸内脂类信号分子指示菌
NTL4 阴性对照
Nti(pTiC5△AACCR) 阳性菌
乳酸菌表达质粒
pORI280 (在EC1000菌中)
pMG36E (在EC1000菌中) 表达型载体
pBABE puro H-Ras V12 | pBS-p85alpha | V143 pLP-HA SD - Acceptor | pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC | pPD177.62 | pUB23-M-beta-gal |
Plasmid | pCMV-mycP35 | V27 pLPS-3 HA - Acceptor | pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC | L4817 | pL-rpb1-C67Y |
pBABE puro cyclinD1 HA | pcDNA3-cdk5GFP | V516 pLP-dMyc - Acceptor | pcDNA3.1/Myc-His(-)-MSulf-1 | L4819 | 3413 pBF339 Bcl-XL |
pBABE-neo largeTcDNA H42Q | pCMV-P10HA | V517 pLP-dMyc SD - Acceptor | pcDNA3.1/Myc-His(-)-MSulf-2 | L4842 | 3410 pBM272 Bcl-XL |
pIRES2-EGFP-SHP2WT | pcDNA3-dnCDK5 | V518 pLPS-3dMyc - Acceptor | IRE1 alpha-pcDNA3.EGFP | L754 | 3405 pBM272 Bid p22 |
1200 pAdTrack FKHR H215R | pcDNA3.1-P25C-GFP | pCMV5B-HA-Smad2 | IRE1 alpha KA-pcDNA3.EGFP | pPD138.11 | 3396 pBM272 Bax |
1130 pBabe puroL HA FKHR H215R AAA | cytosolic moPrP (3F4) | pPRIME-TET-GFP-recipient | WFS1-Flag-pcDNA3 | pPD176.36 | pKT0178 |
1068 pGL3 FasL promoter (no SV40 prom) | moPrP(3F4) D177N | pPRIME-TET-GIN-recipient | WFS1 P724L-Flag-pcDNA3 | pPD172.01 | pKT0176 |
1121 pcDNA3 GFP FKHR H215R | pcDNA-GATA3-DNzf | pPRIME-CMV-GFP-recipient | GFP-PTEN | pPD177.71 | pKT0146 |
1120 pBabe puroL HA FKHR H215R | pcDNA-GATA2-DC+Nzf | pPRIME-CMV-dsRed-recipient | EGFP-supervillin | pLT63 | pKT0150 |
1119 pBabe puroL HA FKHR AAA | pcDNA-GATA2-DNzf | pPRIME-CMV-Neo-recipient | DsRed-Rab5 WT | pLT61 | pKT0149 |
1098 pcDNA GFP FKHR | pcDNA-GATA2-DCzf | pPRIME-CMV-LNGFR-recipient | DsRed-Rab5 DN | pJKL464 | pKT0240 |
1477 pcDNA3 flag HA Akt1 | pGEX2T-GATA3 | pPRIME-TREX-GFP-recipient | mTLR1 | pBK125 | pKT0239 |
1272 pBabe puroL Myr HA Akt3 | Plasmid | pPRIME-TET-GFP-FF3 | pmCherry-ATG5 | L4816 | pKT0233 |
1236 pcDNA3 Myr HA Akt3 | pMTH PKC alpha | pPRIME-CMV-LNGFR-FF3 | pLB | L4809 | pKT0220 |
1227 pcDNA3 Myr HA Akt2 | YFP-CD82 | pCMV5-Smad7-HA | pLB-915 | L4796 | pKT0211 |
1072 pBabe puroL myr Akt K179M T308A S473A | cdk5 T33/pcmv | H2B-GFP | hTLR1 flag | L4781 | pKT0175 |
1071 pBabe puroL myr Akt T308A S473A | pMT ERK1 | RV-L3-HyTK-2L | hTLR2 flag | L4780 | pKT0140 |
1017 pcDNA3 Myr Akt1 deltaPH | HA-FOXO3a TM | pL3-TRE-LucGFP-2L | mTLR2 | L4759 | pKT0139 |
1036 pcDNA3 Myr HA Akt1 | HA-FOXO3a WT | pL3-TRE-MCS-polyA-2L | hTLR3 flag | L4753 | pKT0209 |
GFP-siRNA control | Twist-siRNA3 | p53-GFP | mTLR4 | L4752 | pKT0128 |
1479 pcDNA3 flag HA 14-3-3 gamma | pBABE-puro-mTwist | pCINeo-mCHMP5 | hTLR4 | L4687 | pKT0127 |
Plasmid | pCI neo-hEST2-HA | pCMV5B-Flag-Smad1 | mTLR4 flag | L4686 | pKT0232 |
pRK7-HA-S6K1-F5A-E389-R3A | pCI neo-hEST2 | pCMV5B-FLAG-SARA dSBD | hTLR5 flag | L4683 | pKT0221 |
pRK7-HA-S6K1-F5A-E389 | pBABE-zeo largeTcDNA | pCMV5B-FLAG-SARA | mTLR6 | L4682 | pKT0212 |
pRK7-HA-S6K1-KR | pBABE-zeo largeTgenomic | pCMV5-ALK2 Q207D | mTLR9 | L4671 | pKT0210 |
pcDNA3-myc-FLNa S2152A | pcdna3.1 hciap2mut | pCMV5-ALK2 wt | MYD88 | L4667 | pKT0102 |
pcDNA3-myc-FLNa WT | PCTAIRE-1-DN-HA | pCMV5B-Flag-Smad3 | MYD88 flag | L4666 | pKT0101 |
pKH3-avian RSK1 K112/464R | PCTAIRE-1-DN | pCMV5B-Smad4 | pmCherry-ATG5-K130R | L4665 | pKT0174 |
pcDNA3-HA-ERK2 WT | PCTAIRE-1 | pCMV5-Smad5 | GADD45 P-2/3m-luc | L4664 | pKT0103 |
pBS/U6 ezrin siRNA | EE-mut SV40 luc | pcDNA3-Myc-Smad8 | pc3XB-ZF1 | L4663 | pKT0090 |
pCI flag PCAF | pAd-Track Flag-SIRT1 H355A | pCMV5B-Flag-Smurf2 wt | pc3XB-ZF2 | L4660 | pRB2963 |
tet H-ras val12 | pAd-Track Flag-SIRT1 | pCMV5B-Flag-Smurf2 C716A | pc3XB-ZF3 | L4643 | pRB2960 |
Gal4 PGC1 alpha L2/3A | pAd-Track Control siRNA | pCMV5B-Flag-Par6 wt | pc3XB-ZF4 | L4640 | pRB2958 |
Gal4 PGC1 alpha 1-550 | Plasmid | pCMV5B-Flag-Par6 S345A | pc3XB-ZF5 | L4506 | pRB2956 |
如有需要请联系:
Dr Guo,Senior Scientist
手机l89OI268599
电邮biovector@163.com
Biovector实验室质粒载体菌株基因细胞株列表网址http://biovector.blog.163.com
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