自 杀重组质粒pKC1139,pJQ200SK,pUTminiTn5,pORI280,大肠杆菌宿主细胞JM101,103,105,107,109,110,TOP10,10F,HB101

• pUT-miniTn5 Cm

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• 图谱Map

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The mini-Tn5 are mobile elements able to transpose to the chromosome from a delivery plasmid, by means of the transposase activity encoded by the tnp* gene, which is present in cis in the plasmid but external to the mini-Tn5 element. pUT mini-Tn5 vectors can be used to insert any DNA fragment in the bacterial chromosome, by cloning it within the mini-Tn5 region. Due to the loss of the tnp* gene after insertion, minitransposons are stably integrated into the chromosome and inherited. The different pUTmini-Tn5 vectors can then be used for repeated insertion events and therefore multiple insertions in the same strain are only limited by the availability of distinct selection markers.

Advantages:

• Easy and stable insertion of heterologous DNA in the chromosome of the strain of interest, after few simple genetic manipulations.

• Wide range of inserted fragment sizes.

• Possibility of multiple insertions in the same strain.

• Easy modification of the cloning vector.

• Use of cleavage sites of the rare cutters NotI and SfiI.

• Wide range of Gram negative recipient strains.

Some of the most common applications of pUTmini-Tn5 vectors are:

• Cloning.

• Insertional mutagenesis.

• Promoter probing.

• Generation of conditional phenotypes and non-polar mutations.

• Analysis of transcriptional terminators.

• Environmental applications: tagging with marker genes for monitoring of released microorganisms and/or transcriptional regulation in the environment.

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• pUT mini-Tn5 Cm kit

  • pUT mini-Tn5 Cm, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn Km kit

  • pUT mini-Tn5 Km, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2073), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn Sm/Sp kit

  • pUT mini-Tn5 Sm/Sp, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn Tc kit

  • pUT mini-Tn5 Tc, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

  PANC-1 mini-Tn5 package

  • pUT mini-Tn5 Km, 2 μg.

  • pUT mini-Tn5 Cm, 2 μg.

  • pUT mini-Tn5 Sm/Sp, 2 μg.

  • pUT mini-Tn5 Tc, 2 μg.

  
  

  PANC-1plus miniTn5 package

  • pUT mini-Tn5 Km, 2 μg.

  • pUT mini-Tn5 Cm, 2 μg.

  • pUT mini-Tn5 Sm/Sp, 2 μg.

  • pUT mini-Tn5 Tc, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • E. coli DH5a(pRK2073), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn5Tel kit

  • pUT mini-Tn5Tel vector, 2 μg.

  • Potassium Tellurite, 100mg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

另有

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