出品公司: | Clontech |
---|---|
载体名称: | pLVX-TRE3G-mCherry |
质粒类型: | 哺乳动物细胞慢病毒表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | TRE3GV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 9115 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄 |
筛选标记: | 嘌呤霉素 |
备注: | 包含有Tet-On 3G反式激活蛋白,多西环素(doxycycline)诱导基因表达的慢病毒载体,含有mCherry荧光蛋白标签。 |
产品目录号: | 631349, 631352, 631355 |
稳定性: | / |
组成型: | 诱导型 |
病毒/非病毒: | 慢病毒 |
pLVX-TRE3G-mCherry慢病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格:¥1920
- 货 号:BiovectorLVX-TRE3G-mCherry
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-TRE3G-mCherry 9115 bp DNA circular 9-MAY-2012 COMMENT Created by Clontech Laboratories Inc. COMMENT 631360, 631349, 631352, 631255 COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|bivector.net| FEATURES Location/Qualifiers misc_feature 1..635 /label=5'\LTR misc_feature 685..822 /label=psi misc_feature 1303..1536 /label=RRE misc_feature 2028..2151 /label=cPPT/CTS misc_feature 3896..4399 /label=pPGK misc_feature 4420..5019 /label=Puro misc_feature 5037..5628 /label=WPRE 3'clip 5832..6468 /label=3'\LTR misc_feature complement(6938..7608) /label=pUC\ori misc_feature 2564..3274 /label=mCherry misc_feature 3283..3871 /label=IRES misc_feature 3872..3895 /label=MCS misc_feature 2209..2560 /label=pTRE3GV misc_feature complement(8614..8718) /label=pAmp(R) misc_feature complement(7753..8613) /label=Amp(R) BASE COUNT 2273 a 2340 c 2410 g 2092 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac 2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga 2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat 2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag 2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc 2521 ctggagcaat tccacaacac ttttgtctta tacttggatc accatggtga gcaagggcga 2581 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 2641 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 2701 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 2761 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 2821 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 2881 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 2941 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 3001 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 3061 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 3121 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 3181 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 3241 ctccaccggc ggcatggacg agctgtacaa gtagggccgc cccggccggc ccctctccct 3301 cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc 3361 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc 3421 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt 3481 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct 3541 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa 3601 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt 3661 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag 3721 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt 3781 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt 3841 ttcctttgaa aaacacgatg ataatatggc ccccgggacg cgtcatatgg aattctaccg 3901 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg 3961 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg 4021 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag 4081 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg 4141 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg 4201 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg 4261 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc 4321 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc 4381 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg 4441 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc 4501 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc 4561 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 4621 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 4681 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 4741 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 4801 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 4861 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 4921 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 4981 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac ggggcgcgtc tggaacaatc 5041 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 5101 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 5161 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 5221 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 5281 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 5341 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 5401 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 5461 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 5521 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 5581 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg 5641 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc 5701 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggtttttcca gtcacacctc 5761 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 5821 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt 5881 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg 5941 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg 6001 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga 6061 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc 6121 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca 6181 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc 6241 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta 6301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 6361 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac 6421 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt 6481 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct 6541 tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata 6601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6721 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 6781 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 6841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 6901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 6961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 7441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7501 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac 7561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 7681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 7741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 7801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 7861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 7921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 7981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 8041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 8101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 8161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 8221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 8281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 8341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 8401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 8461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 8521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 8581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 8641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 8701 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg 8761 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 8821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 8881 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc 8941 ccaccccata ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt 9001 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac 9061 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt //
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