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pLX302 BioVector NTCC质粒载体菌种细胞基因保藏中心
Vector backbone pLKO
(Search Vector Database)
Backbone size (bp) 9573
Vector type Mammalian Expression, Lentiviral ; Gateway Destination vector
Selectable markers Puromycin
GROWTH IN BACTERIA
Bacterial Resistance(s) Chloramphenicol and Ampicillin
Growth Temperature 30°C
Growth Strain(s) ccdB Survival
Growth instructions ccdB resistant bacteria. 30C for liquid culture (slow grower--allow 24 hours for growth). 37C for plates.
Copy number Low Copy
GENE/INSERT
Gene/Insert name None
Tag / Fusion Protein
V5 (C terminal on backbone)
CLONING INFORMATION
Cloning method Gateway Cloning
5′ sequencing primer CMV-F
3′ sequencing primer WPRE-R
There is a C-terminal V5 tag followed by a Stop codon directly after the Gateway sequence.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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