E. coli BW25113 BioVector NTCC质粒载体菌种细胞基因保藏中心

E. coli BW25113

The E. coli K-12BW25113 parent strain is the common background genotype used for the generationof the E. coli Keio Knockout Collection, which is a set of precisely defined,single-gene deletions of all nonessential genes in E.coli K-12. Out of 4288genes targeted, mutants were obtained for 3985 genes. Two independent mutantswere obtained for each deleted gene, yielding a total of 7970 knockout strains(Baba et al, 2006).

Using λ Red recombination, aFRT-flanked kanamycin cassette was utilized to replace each codingregion. This cassette may be excised by FLP recombination, leaving an in-frame,translatable sequence that includes the endogenous start, a shortrecombinational scar sequence, and an 18-nucleotide, C-terminal coding sequencefrom the endogenous gene. For details concerning cassette excision, pleaseconsult (Datsenko and Wanner, 2000).

This collection is anew resource for systematic analyses of unknown gene functions and generegulatory networks, but also for genome-wide testing of mutational effects ina common strain background.

Genotype of BW25113:

rrnB3 ΔlacZ4787 hsdR514Δ(araBAD)567 Δ(rhaBAD)568 rph-1.

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