胞嘧啶脱氨酶缺陷型大肠杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

胞嘧啶脱氨酶缺陷型大肠杆菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心 E.coli cytosine deaminase knock-out strain
Hazardous No
Shipping Condition Dry Ice,Frozen Gel Packs
Stability at Recommended Storage Conditions At least 12 months
Storage Condition -80 C
Clones are provided as a live culture in a 2 mL tube. Each tube contains LB medium supplemented with 8% glycerol and kanamycin. Within 2 to 3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C.
Gene knockout primers have 20- to 30-nucleotide ends for priming upstream (P1) and downstream (P2) of the FRT sites flanking the kanamycin resistance gene in pKD13 and 50-nucleotide ends homologous to upstream (H1) and downstream (H2) chromosomal sequences for targeting the gene deletion. H1 includes the gene B (target) initiation codon. H2 includes codons for the six C-terminal residues, the stop codon, and 29 nucleotides downstream. The same primer design with respect to gene B was used to target deletions regardless of whether gene B lies in an operon with genes A and C, as shown, or in different chromosomal arrangements. Novel junctions created between the resistance cassette and neighboring upstream (gene A) and downstream (gene C) sequences were verified by PCR with kanamycin (k1 or k2) and locus-specific (U or D) primers. Structures created after excision of the resistance gene are verified by PCR with neighboring gene-specific primers and by direct DNA sequencing of the region encompassing the H1-P1-FRT-P2-H2 scar to verify correct ones, as described elsewhere (Datsenko and Wanner, 2000). SD, Shine–Dalgarno ribosome binding sequence. (Baba et al., 2004).

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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