RIVA cells淋巴瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

RIVA cells淋巴瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 RIVA cells淋巴瘤细胞株
Cell line: RI-1
Species: human (Homo sapiens)Cell type: B cell lymphoma Origin: established from the peripheral blood of a 57-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL, lymphocytic, small cell type progressing to large non-convoluted cell type) in the refractory terminal stage in 1977; assigned to ABC-like lymphoma subtype (activated B-cell); cell line also known as Riva Reference(s): 15013, 17354Biosafety level: 1 Permissions and restrictions: A
Cell Culture Data: Morphology:large round cells growing in suspension; image; image; imageMedium: 90% RPMI 1640 + 10% h.i. FBS Subculture: seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca. 1-2 x 106 cells/ml; maximal density at ca. 3-4 x 106 cells/ml Incubation: at 37 °C with 5% CO2 Doubling time: ca. 48-72 hours Harvest: maximum density at about 3-4 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO
Scientific Data: Mycoplasma: negative in microbiological culture, PCR assays Immunology: CD3 -, CD10 -, CD13 -, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile Species: confirmed as human by cytogenetics and species PCR Cytogenetics: human flat-moded hyperdiploid karyotype with 4% polyploidy - 48-51<2n>XX, +7, +18, +19, +mar, t(1;22;16)(p35;p12;q12.2), del(3)(q11-12), trp(3)(q11-12q28-29), t(4;8)(q22;q24), del(6)(q13), der(15)t(13;15)(q11;p1?1), der(18)amp(18)(q21)dup(18)(q21q23), der(19)t(6;19)(p21;p13)add(19(q13.3) - carries t(4;8) and der(18) effecting MYC rearrangement and amplification of BCL2, respectively - resembles published karyotype Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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