pSR47S自杀质粒细菌基因置换载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSR47S自杀质粒细菌基因置换载体

BioVector NTCC质粒载体菌种细胞基因保藏中心  

pSR47S自杀质粒细菌基因置换载体，带有R6K自杀型复制子，需要lambda-pir型宿主菌扩增，如DH5a-lambda pir, S17-lambda pir，Cm抗性，sacB蔗糖致死基因。
Bacterial Expression ; Bacterial allelic exchange vector with sacB
Contains lambda pir-dependent R6K replication origin; requires lambda pir-containing bacteria strain, e.g. DH5a-lambda pir, S17-lambda pir
Copy number Low Copy
Plasmid pSR47S Cm was constructed by removing the KmR region from pSR47S by PCR amplification of the residual plasmid, then cloning in the CmR gene. The CmR gene was amplified from a pMMB207 plasmid by PCR, with restriction sites that allowed cloning into pSR47S that lacked the KmR region.

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