BA/F3 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

BA/F3 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心                      BA/F3 cells细胞株
Species: murine (mouse) (Mus musculus)Cell type: pro B cells Origin: IL-3 dependent murine pro B cell line; the precise origin of the cell line is unclear; however, performing May-Grünwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic back ground. Cells were apparently not derived from BALB/c, but from C3H mouse. Direct publication is not available, but for related publications see references. Reference(s): 14829, 14830Biosafety level: 1 Permissions and restrictions: A  DSMZ Cell Culture Data: Morphology:mostly single, round (some polymorph) cells in suspension (or occasionally in clumps); image; image; imageMedium: 90% RPMI 1640 + 10% h.i. FBS + 10 ng/ml mouse IL-3 or 10% vol conditioned medium of WEHI-3B cell line (DSM ACC 26) Subculture: split saturated culture 1:10 every 3 days; seed out at about 1-3 x 105 cells/ml; at high cell density (>2 x 106 cells/ml), a cytokine-independent subclone may grow out relatively quickly. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. Incubation: at 37 °C with 5% CO2 Doubling time: about 20 hours Harvest: maximal density of about 2.5 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO  DSMZ Scientific Data: Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays Immunology: CD11b (+), F4/80 (+); image Species: originally confirmed as mouse with IEF of AST, MDH, NP, PEP B and with species PCR and recently as mus musculus by COI DNA Barcoding Cytogenetics: murine near diploid karyotype with 33% polyploidy - 40(36-42)<2n> Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
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