pJOE8999枯草芽孢杆菌基因编辑载体CRISPR-Cas9系统 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥28935
- 货 号:pJOE8999枯草芽孢杆菌基因编辑载体CRISPR-Cas9系统
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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pJOE8999枯草芽孢杆菌基因编辑载体CRISPR-Cas9系统
BioVector NTCC质粒载体菌种细胞基因保藏中心
pJOE8999枯草芽孢杆菌基因编辑载体CRISPR-Cas9系统,pUC18复制起始位点,温度敏感型pE194ts复制子,Kan抗性基因,Pman启动子+Cas9基因,PvavP启动子+sgRNA向导序列,lacPOZ',λ oop终止子,以及T7启动子。
CRISPR-Cas9 vector pJOE8999. (A) Physical map of vector pJOE8999 containing the pUC18 minimal origin, the temperature-sensitive replication origin from pE194ts, a kanamycin resistance gene (kanR), cas9 under the control of the PmanP promoter, the sgRNA transcribed from the semisynthetic promoter PvanP* interrupted by the lacZ α fragment (lacPOZ'), the λ oop terminator, and the T7 promoter (T7P). (B) Cloning sites in the vector pJOE8999. (C) Promoter sequence (PvanP*) upstream of the sgRNA with the −35 and −10 sequences and the BsaI restriction sites (bold) for insertion of the spacer sequence.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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