MCF- 7/Luc Cell Line荧光稳定表达细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

MCF- 7/Luc Cell Line荧光稳定表达细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 MCF- 7/Luc Cell Line
CATALOG NUMBER: AKR-234
STORAGE: Liquid nitrogen
Note: For best results begin culture of cells immediately upon receipt. If
this is not possible, store at -80ºC until first culture. Store subsequent
cultured cells long term in liquid nitrogen.
QUANTITY & CONCENTRATION: 1 mL, 1 x 106 cells/mL in 70% DMEM, 20% FBS, 10% DMSO
MCF-7 is a human breast cancer cell line that was first isolated in 1970 from the malignant
adenocarcinoma breast tissue of a 69-year old woman. MCF-7 is the acronym of Michigan Cancer
Foundation - 7, referring to the institute in Detroit where the cell line was established. MCF-7 cells are
useful for in vitro breast cancer studies because the cell line has retained several ideal characteristics
particular to the mammary epithelium. These include the ability for MCF-7 cells to process estrogen via
estrogen receptors. MCF-7 cells are also sensitive to cytokeratin. When grown in vitro, the cell line is
capable of forming domes and the epithelial like cells grow in monolayers. Growth can also be inhibited
using tumor necrosis factor alpha (TNF alpha). Our MCF-7/Luc cell line stably expresses firefly
luciferase gene and Neomycin resistant gene.
Background
Figure 1. MCF-7/Luc Cell Line. Left: Phase Contrast; Right: Luciferase Activity Assay.
This cryovial contains at least 1.0 × 106 MCF-7/Luc cells as determined by morphology, trypan-blue dye
exclusion, and viable cell count. The MCF-7/Luc cells are tested free of microbial contamination.
Quality Control
1. Culture Medium: D-MEM (high glucose), 10% fetal bovine serum (FBS), 0.1 mM MEM NonEssential
Amino Acids (NEAA), 2 mM L-glutamine, 1% Pen-Strep.
Medium
2. Freeze Medium: 70% DMEM, 20% FBS, 10% DMSO.
Establishing MCF-7/Luc Cultures from Frozen Cells
Methods
1. Place 10 mL of complete DMEM growth medium in a 50-mL conical tube. Thaw the frozen
cryovial of cells within 1–2 minutes by gentle agitation in a 37°C water bath. Decontaminate the
cryovial by wiping the surface of the vial with 70% (v/v) ethanol.
2. Transfer the thawed cell suspension to the conical tube containing 10 ml of growth medium.
3. Collect the cells by centrifugation at 1000 rpm for 5 minutes at room temperature. Remove the
growth medium by aspiration.
4. Resuspend the cells in the conical tube in 15 mL of fresh growth medium by gently pipetting up
and down.
5. Transfer the 15 mL of cell suspension to a T-75 tissue culture flask. Place the cells in a 37°C
incubator at 5% CO2.
6. Monitor cell density daily. Cells should be passaged when the culture reaches 95% confluence.
1. Sridhar, S.S. et al. (2016). Tracking the dynamics or circulating tumour cell phenotypes using
nanoparticle-mediated magnetic ranking. Nature Nanotech. doi:10.1038/nnano.2016.239.
Recent Product Citations
2. Sardo, C. et al. (2016). Improvements in rational design strategies of inulin derivative polycation
for siRNA delivery. Biomacromolecules. doi:10.1021/acs.biomac.6b00281.
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