MOLM-16 cells BioVector NTCC质粒载体菌种细胞基因保藏中心

MOLM-16 cells BioVector NTCC质粒载体菌种细胞基因保藏中心 Cell line:
MOLM-16
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established in 1999 from the peripheral blood of a 77-year-old Japanese woman at relapse of acute myeloid leukemia (AML M0) after failed chemotherapy
Biosafety level:
1
Permissions and restrictions:
A

NTCC Cell Culture Data:
Morphology:
large round cells growing singly or in small clumps in suspension; image;


Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml in 24-well-plate; once established in culture maintain at ca 0.1-0.8 x x106 cells/ml; split saturated culture 1:2 to 1:10 every 2-5 days; reaches maximum density of 1 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-80 hours
Harvest:
cell harvest of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD13 +, CD14 (+), CD19 -, CD33 +, CD34 +, CD71 +, CD235a +;
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human by PCR speciation and cytogenetics
Cytogenetics:
human hypotetraploid karyotype with hypodiploid sideline - 83-88<4n>XXX, -X, -4, -4, -5, +6, +7, -9, -18, +2mar, add(5)(q11)x2, add(6)(q11-13), der6)t(6;9(q16;p13), t(6;8)(q15;q24.3), der(8)t(6;8)(q15;q24.3), der(9;18)(p10;q10), der(16)del(16)(p13)add(16)(q11), der(16)(del16)(p13)dup(16) (q?11q?24)ins(16;?)(q11;??), der(18)t(9;18)(q13;q21), der(19)add(19)(p13)add(19)(q13)x2 - resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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