pHULK piggyBac N-CometGFP转座子彗星荧光表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pHULK piggyBac N-CometGFP BioVector NTCC质粒载体菌种细胞基因保藏中心

pHULK piggyBac N-CometGFP转座子彗星荧光表达载体，表达piggybac转座酶，puro筛选标记，EF1a启动子表达CometGFP绿色荧光和目的基因融合表达，Kan抗性。
Description: The piggyBac transposon system relies on the ability of transiently produced
piggyBac transposase to efficiently integrate a defined region of plasmid vector
into genomic TTAA sites. The portion of the plasmid coding for piggyBac
transposase is looped out and degraded, thus allowing for the production of cell
lines with stable integration of transgene(s). An optimal configuration of
promoter and expression enhancement elements ensures that a pool of
antibiotic selected cells can be immediately used for functional assays.
Catalog Number: pJ519-02
Package Amount: 2 μg
Mammalian Marker: Puromycin
Mammalian Promoter: hEF1alpha
Bacterial Marker: Kanamycin
Bacterial Origin: pUC (High Copy)
Fluorescent Protein: Comet Green Fluorescent Protein, excitation at 403nm and emission at 511nm.
Polylinker Cloning Information:
CometGFP lacks an immediate stop codon but is instead followed by a polylinker for the option to clone an
ORF of interest in-frame with an N-terminal CometGFP.
Downstream of the N-terminal CometGFP ORF there are several unique restriction sites available for cloning;
it is critical to preserve the in-frame translation with the CometGFP.


同类别载体还有：

pJ509-02 pHULK piggyBac Mammalian Expression Vector CometGFP, Puromycin
品番 pJ509-01 pHULK piggyBac Mammalian Expression Vector Purple Prancer Insert, Puromycin
品番 pJ519-02 pHULK piggyBac Mammalian Expression Vector N-terminal CometGFP, Puromycin
品番 pJ539-02 pHULK piggyBac Mammalian Expression Vector C-terminal CometGFP, Puromycin
品番 pJ529-02 pHULK piggyBac Mammalian Expression Vector N-terminal CometGFP, Puromycin
pJ508-02 pHULK piggyBac Mammalian Expression Vector CometGFP, Blasticidin
pJ500-00 pHULK Excision Vector
品番 pJ547-17 pHULK piggyBac Mammalian Expression Vector RudolphRFP-IRES-CometGFP, Hygromycin
品番 pJ548-17 pHULK piggyBac Mammalian Expression Vector RudolphRFP-IRES-CometGFP, Blasticidin
品番 pJ549-17 pHULK piggyBac Mammalian Expression Vector RudolphRFP-IRES-CometGFP, Puromycin
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品番 FPB-20-509 CindyLouCFP, IP-Freeⓒ Fluorescent ProteinPaintboxTM - Mammalian, piggyBac_EF1a
品番 FPB-26-509 CometGFP, IP-Freeⓒ Fluorescent ProteinPaintboxTM - Mammalian, piggyBac_EF1a
品番 FPB-27-509 DasherGFP, IP-Freeⓒ Fluorescent ProteinPaintboxTM - Mammalian, piggyBac_EF1a
品番 FPB-30-509 YukonOFP, IP-Freeⓒ Fluorescent ProteinPaintboxTM - Mammalian, piggyBac_EF1a
品番 FPB-31-509 RudolphRFP, IP-Freeⓒ Fluorescent ProteinPaintboxTM - Mammalian, piggyBac_EF1a
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
[Supplier来源]BioVector NTCC Inc.
【Website网址】 http://www.biovector.net