Cell line YT细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Cell line YT细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
YT
NTCC no.:
NTCC-ACC434
Species:
human (Homo sapiens)
Cell type:
T/NK cell leukemia
Origin:
established from the pericardial fluid of a 15-year-old man with acute lymphoblastic leukemia (ALL) at relapse (with accompanying thymoma) in 1983; cells were described as: (i) showing NK activity and ADCC (antigen-dependent cell-mediated cytotoxicity), (ii) having their T cell receptor (TCR) genes in germline configuration, and (iii) expressing no TCR proteins (also CD3-, CD16-, CD56+, CD57-)
Reference(s):
14487, 14647, 15072, 15074
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.

NTCC Cell Culture Data:
Morphology:
round to polygonal cells growing singly or in clumps in suspension; image
Medium:
80% Iscove's MDM + 20% h.i. FBS
Subculture:
split saturated culture 1:2 every second day; start culture in a 24-well-plate and seed out at ca. 0.8-1.5 x 106 cells/ml, initially after thawing the viability may be reduced down to ca. 20-50% during the first 1-2 weeks (during that initial period, cells should not be diluted, medium may be exchanged without centrifugation); in their vigorous growth phase, maintain at 0.2-0.5 x 106 cells/ml; maximal density at ca. 0.6-0.8 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-50 hours
Harvest:
cell harvest of ca. 0.6 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD2 -, CD3 -, CD4 -, CD5 -, CD6 -, CD7 +, CD8 -, CD13 -, CD19 -, CD25 (+), CD30 +, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human with IEF of AST, LDH, NP
Cytogenetics:
human flat-moded near-tetraploid karyotype with 12% polyploidy - 84-98<4n>XXYY, -6, -9, -15, -19, +6-8mar, der(X)t(X;7)(q25;q21), der(1)dup(1)(q12.2q2?2)t(1;17)(q3?1;q1?2), der(4)t(1;4)(q32.2;q35)x2, der(5)t(X;5)(p1?3;q21)x2, del(6)(p11), der(10;17)(q10;q10), dup(14)(q1?q2?), add(16)(q24), add(17)(q11) - resembles published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, MLV -, SMRV -

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net