出品公司: | Addgene |
---|---|
载体名称: | pLOX-TERT-iresTK |
质粒类型: | 慢病毒系统载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 13117bp |
5' 测序引物及序列: | CMV fwd: CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | SP6: ATTTAGGTGACACTATAG |
载体标签: | -- |
载体抗性: | 氨苄青霉素(Ampicillian) |
筛选标记: | 博来霉素(Zeocin) |
备注: | 克隆菌株为Stbl3和HB101。 |
产品目录号: | 12245 |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | 慢病毒 |
pLOX-TERT-iresTK慢病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格:¥1920
- 货 号:BiovectorLOX-TERT-iresTK
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
CMV promoter is upstream of the bicistronic coding cassette with hTert and HSV1-TK. During reverse transcription U3 region of the 3'LTR, with LoxP, is duplicated; LoxP sites end up flanking the genome of the integrated provirus. Upon expression of Cre, provirus is excised.
载体序列
LOCUS pLOX-TERT-iresTK 13117 bp DNA SYN DEFINITION pLOX-TERT-iresTK ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..13117 /organism="pLOX-TERT-iresTK" /mol_type="other DNA" misc_feature 457..637 /label="HIV-1_5_LTR" misc_feature 457..637 /label="truncHIV-1_3_LTR" misc_feature 748..792 /label="HIV-1_psi_pack" CDS 1180..2028 /label="ORF frame 1" misc_feature 1302..1535 /label="RRE" promoter 2040..2544 /label="CMV_immearly_promoter" misc_feature 2047..2334 /label="CAG_enhancer" misc_feature 2501..2521 /label="CMV_fwd_primer" promoter 2502..2571 /label="CMV_promoter" misc_feature 2545..2564 /label="pCEP_fwd_primer" misc_feature 2547..2571 /label="LNCX_primer" CDS complement(2622..3305) /label="ORF frame 3" CDS 2691..6089 /label="ORF frame 3" CDS complement(3726..4946) /label="ORF frame 3" misc_feature 6118..6608 /label="IRES" CDS 6612..7565 /label="ORF frame 3" misc_feature 7609..7630 /label="U3PPT" misc_feature 7609..7624 /label="cPPT" misc_feature 7681..7714 /label="loxP" misc_feature 7734..7914 /label="HIV-1_5_LTR" misc_feature 7734..7914 /label="truncHIV-1_3_LTR" misc_feature complement(7940..7957) /label="Sp6_primer" gene 8195..8458 /label="tet (300 - 563)" /gene="tet (300 - 563)" misc_feature complement(8247..8266) /label="pBRrevBam_primer" misc_feature 8599..8621 /label="pGEX_3_primer" promoter 8780..8808 /label="AmpR_promoter" gene 8850..9710 /label="Ampicillin" /gene="Ampicillin" CDS 8850..9710 /label="ORF frame 3" rep_origin 9865..10484 /label="pBR322_origin" misc_feature complement(10710..10730) /label="pBABE_3_primer" misc_feature complement(10716..11169) /label="SV40_enhancer" promoter 10728..10996 /label="SV40_promoter" rep_origin 10895..10972 /label="SV40_origin" misc_feature 10957..10976 /label="SV40pro_F_primer" misc_feature 12177..12192 /label="SV40_int" misc_feature 12198..12245 /label="SV40_3_splice" CDS 12322..12795 /label="ORF frame 1" misc_feature 12373..12393 /label="SV40 NLS" misc_feature 12379..12393 /label="NLS" terminator 12821..12952 /label="SV40_PA_terminator" misc_feature 12909..12928 /label="EBV_rev_primer" ORIGIN 1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc 61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc 121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg 181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc 241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc 301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt 361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca 421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc 481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga 661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca 721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct 781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat 841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat 901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag 961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta 1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag 1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac 1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt 1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca 1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg 1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc 1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg 1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg 1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa 1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag 1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta 1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg 1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat 1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta 1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc 1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac 1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataagc ttgggagttc 2041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 2101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 2161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 2221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 2281 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 2341 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg 2401 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 2461 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 2521 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga 2581 cgccatccac gctgttttga cctccataga agacaccgac tctagctaga ggatctacta 2641 gtgaattcct gctgcgcacg tgggaagccc tggccccggc cacccccgcg atgccgcgcg 2701 ctccccgctg ccgagccgtg cgctccctgc tgcgcagcca ctaccgcgag gtgctgccgc 2761 tggccacgtt cgtgcggcgc ctggggcccc agggctggcg gctggtgcag cgcggggacc 2821 cggcggcttt ccgcgcgctg gtggcccagt gcctggtgtg cgtgccctgg gacgcacggc 2881 cgccccccgc cgccccctcc ttccgccagg tgtcctgcct gaaggagctg gtggcccgag 2941 tgctgcagag gctgtgcgag cgcggcgcga agaacgtgct ggccttcggc ttcgcgctgc 3001 tggacggggc ccgcgggggc ccccccgagg ccttcaccac cagcgtgcgc agctacctgc 3061 ccaacacggt gaccgacgca ctgcggggga gcggggcgtg ggggctgctg ttgcgccgcg 3121 tgggcgacga cgtgctggtt cacctgctgg cacgctgcgc gctctttgtg ctggtggctc 3181 ccagctgcgc ctaccaggtg tgcgggccgc cgctgtacca gctcggcgct gccactcagg 3241 cccggccccc gccacacgct agtggacccc gaaggcgtct gggatgcgaa cgggcctgga 3301 accatagcgt cagggaggcc ggggtccccc tgggcctgcc agccccgggt gcgaggaggc 3361 gcgggggcag tgccagccga agtctgccgt tgcccaagag gcccaggcgt ggcgctgccc 3421 ctgagccgga gcggacgccc gttgggcagg ggtcctgggc ccacccgggc aggacgcgtg 3481 gaccgagtga ccgtggtttc tgtgtggtgt cacctgccag acccgccgaa gaagccacct 3541 ctttggaggg tgcgctctct ggcacgcgcc actcccaccc atccgtgggc cgccagcacc 3601 acgcgggccc cccatccaca tcgcggccac cacgtccctg ggacacgcct tgtcccccgg 3661 tgtacgccga gaccaagcac ttcctctact cctcaggcga caaggagcag ctgcggccct 3721 ccttcctact cagctctctg aggcccagcc tgactggcgc tcggaggctc gtggagacca 3781 tctttctggg ttccaggccc tggatgccag ggactccccg caggttgccc cgcctgcccc 3841 agcgctactg gcaaatgcgg cccctgtttc tggagctgct tgggaaccac gcgcagtgcc 3901 cctacggggt gctcctcaag acgcactgcc cgctgcgagc tgcggtcacc ccagcagccg 3961 gtgtctgtgc ccgggagaag ccccagggct ctgtggcggc ccccgaggag gaggacacag 4021 acccccgtcg cctggtgcag ctgctccgcc agcacagcag cccctggcag gtgtacggct 4081 tcgtgcgggc ctgcctgcgc cggctggtgc ccccaggcct ctggggctcc aggcacaacg 4141 aacgccgctt cctcaggaac accaagaagt tcatctccct ggggaagcat gccaagctct 4201 cgctgcagga gctgacgtgg aagatgagcg tgcggggctg cgcttggctg cgcaggagcc 4261 caggggttgg ctgtgttccg gccgcagagc accgtctgcg tgaggagatc ctggccaagt 4321 tcctgcactg gctgatgagt gtgtacgtcg tcgagctgct caggtctttc ttttatgtca 4381 cggagaccac gtttcaaaag aacaggctct ttttctaccg gaagagtgtc tggagcaagt 4441 tgcaaagcat tggaatcaga cagcacttga agagggtgca gctgcgggag ctgtcggaag 4501 cagaggtcag gcagcatcgg gaagccaggc ccgccctgct gacgtccaga ctccgcttca 4561 tccccaagcc tgacgggctg cggccgattg tgaacatgga ctacgtcgtg ggagccagaa 4621 cgttccgcag agaaaagagg gccgagcgtc tcacctcgag ggtgaaggca ctgttcagcg 4681 tgctcaacta cgagcgggcg cggcgccccg gcctcctggg cgcctctgtg ctgggcctgg 4741 acgatatcca cagggcctgg cgcaccttcg tgctgcgtgt gcgggcccag gacccgccgc 4801 ctgagctgta ctttgtcaag gtggatgtga cgggcgcgta cgacaccatc ccccaggaca 4861 ggctcacgga ggtcatcgcc agcatcatca aaccccagaa cacgtactgc gtgcgtcggt 4921 atgccgtggt ccagaaggcc gcccatgggc acgtccgcaa ggccttcaag agccacgtct 4981 ctaccttgac agacctccag ccgtacatgc gacagttcgt ggctcacctg caggagacca 5041 gcccgctgag ggatgccgtc gtcatcgagc agagctcctc cctgaatgag gccagcagtg 5101 gcctcttcga cgtcttccta cgcttcatgt gccaccacgc cgtgcgcatc aggggcaagt 5161 cctacgtcca gtgccagggg atcccgcagg gctccatcct ctccacgctg ctctgcagcc 5221 tgtgctacgg cgacatggag aacaagctgt ttgcggggat tcggcgggac gggctgctcc 5281 tgcgtttggt ggatgatttc ttgttggtga cacctcacct cacccacgcg aaaaccttcc 5341 tcaggaccct ggtccgaggt gtccctgagt atggctgcgt ggtgaacttg cggaagacag 5401 tggtgaactt ccctgtagaa gacgaggccc tgggtggcac ggcttttgtt cagatgccgg 5461 cccacggcct attcccctgg tgcggcctgc tgctggatac ccggaccctg gaggtgcaga 5521 gcgactactc cagctatgcc cggacctcca tcagagccag tctcaccttc aaccgcggct 5581 tcaaggctgg gaggaacatg cgtcgcaaac tctttggggt cttgcggctg aagtgtcaca 5641 gcctgtttct ggatttgcag gtgaacagcc tccagacggt gtgcaccaac atctacaaga 5701 tcctcctgct gcaggcgtac aggtttcacg catgtgtgct gcagctccca tttcatcagc 5761 aagtttggaa gaaccccaca tttttcctgc gcgtcatctc tgacacggcc tccctctgct 5821 actccatcct gaaagccaag aacgcaggga tgtcgctggg ggccaagggc gccgccggcc 5881 ctctgccctc cgaggccgtg cagtggctgt gccaccaagc attcctgctc aagctgactc 5941 gacaccgtgt cacctacgtg ccactcctgg ggtcactcag gacagcccag acgcagctga 6001 gtcggaagct cccggggacg acgctgactg ccctggaggc cgcagccaac ccggcactgc 6061 cctcagactt caagaccatc ctggactgat ggcgtcgaca ttaattccgg ttattttcca 6121 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga 6181 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga 6241 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca 6301 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag 6361 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa 6421 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac 6481 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga 6541 ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg 6601 atgataatac catggcttcg tacccctgcc atcaacacgc gtctgcgttc gaccaggctg 6661 cgcgttctcg cggccatagc aaccgacgta cggcgttgcg ccctcgccgg cagcaagaag 6721 ccacggaagt ccgcctggag cagaaaatgc ccacgctact gcgggtttat atagacggtc 6781 ctcacgggat ggggaaaacc accaccacgc aactgctggt ggccctgggt tcgcgcgacg 6841 atatcgtcta cgtacccgag ccgatgactt actggcaggt gctgggggct tccgagacaa 6901 tcgcgaacat ctacaccaca caacaccgcc tcgaccaggg tgagatatcg gccggggacg 6961 cggcggtggt aatgacaagc gcccagataa caatgggcat gccttatgcc gtgaccgacg 7021 ccgttctggc tcctcatatc gggggggagg ctgggagctc acatgccccg cccccggccc 7081 tcaccctcat cttcgaccgc catcccatcg ccgccctcct gtgctacccg gccgcgcgat 7141 accttatggg cagcatgacc ccccaggccg tgctggcgtt cgtggccctc atcccgccga 7201 ccttgcccgg cacaaacatc gtgttggggg cccttccgga ggacagacac atcgaccgcc 7261 tggccaaacg ccagcgcccc ggcgagcggc ttgacctggc tatgctggcc gcgattcgcc 7321 gcgtttacgg gctgcttgcc aatacggtgc ggtatctgca gggcggcggg tcgtggcggg 7381 aggattgggg acagctttcg gggacggccg tgccgcccca gggtgccgag ccccagagca 7441 acgcgggccc acgaccccat atcggggaca cgttatttac cctgtttcgg gcccccgagt 7501 tgctggcccc caacggcgac ctgtataacg tgtttgcctg ggctttggct cgacggtacc 7561 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 7621 gggactggaa gggctaattc actcccaacg aagacaagat gggatcaatt caccatggga 7681 ataacttcgt atagcataca ttatacgaag ttatgctgct ttttgcttgt actgggtctc 7741 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 7801 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 7861 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct 7921 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt 7981 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc 8041 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt 8101 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc 8161 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac 8221 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg 8281 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt 8341 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct 8401 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg 8461 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac 8521 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 8581 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 8641 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 8701 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 8761 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 8821 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 8881 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 8941 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 9001 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 9061 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 9121 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 9181 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 9241 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 9301 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 9361 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 9421 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 9481 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 9541 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 9601 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 9661 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 9721 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 9781 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 9841 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 9901 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 9961 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 10021 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 10081 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 10141 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 10201 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 10261 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 10321 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 10381 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 10441 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 10501 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 10561 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 10621 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 10681 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc 10741 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 10801 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 10861 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 10921 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 10981 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 11041 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg 11101 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg 11161 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag 11221 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc 11281 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta 11341 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg 11401 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt 11461 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac 11521 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt 11581 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac 11641 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta 11701 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat 11761 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc 11821 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg 11881 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca 11941 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc 12001 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga 12061 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct 12121 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta 12181 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt 12241 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa 12301 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa 12361 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa 12421 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt 12481 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta 12541 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac 12601 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta 12661 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat 12721 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct 12781 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa 12841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 12901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat 12961 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa 13021 ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa 13081 gaaattgtat ttgttaaata tgtactacaa acttagt //
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