E. coli MC4100/pTGM BioVector NTCC质粒载体菌种细胞基因保藏中心

E. coli MC4100/pTGM BioVector NTCC质粒载体菌种细胞基因保藏中心
An extended range whole-cell tetracycline biosensor strain was constructed by insertion of the tet(M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid pTGFP2, which contains a transcriptional fusion between a tetracycline regulated promoter and the green fluorescent protein gene. Tetracycline, oxytetracycline, chlortetracycline and minocycline all effectively induced the resulting Escherichia coli MC4100/pTGM biosensor and similar dose–response characteristics were recorded by flow cytometry for all four compounds. The novel tetracycline biosensor was responsive to drug concentrations ranging from below 5 ng ml−1 to 16 μg ml−1, which represents a significant improvement of the original version.
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