HL-1 Cardiac Muscle Cell Line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HL-1 Cardiac Muscle Cell Line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
HL-1 Cardiac Muscle Cell Line
Cat No.: NTCC595617

HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion.

The HL-1 line was derived from AT-1 subcutaneous tumor excised from an adult female C57BL/6J mouse. The parental AT-1 line was originally derived from an atrial tumor growing in a transgenic mouse in which expression of the SV40 large T-antigen was targeted to atrial cardiomyocytes via the atrial natriuretic factor (ANF) promoter.

HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels.

Storage conditions  
HL-1 Cardiac Muscle cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

References:
1. Claycomb, W.C., Lanson, N.A. Jr., Stallworth, B.S., Egeland, D.B., Delcarpio, J.B., Bahinski, A., Izzo, N.J. Jr. (1998). HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte. Proc. Natl. Acad. Sci. USA. 95(6): 2979-2984.
2. White, S. M., Constantin, P. E., & Claycomb, W. C. (2004). Cardiac physiology at the cellular level: use of cultured HL-1 cardiomyocytes for studies of cardiac muscle cell structure and function. American Journal of Physiology-Heart and Circulatory Physiology, 286(3), H823-H829.

Supplemented Claycomb Medium
Product Name ml Final Concentration
Claycomb Medium 87  
Fetal Bovine Serum 10 10%
Penicillin/Streptomycin 1 100 U/ml:100 µg/ml
Norepinephrine (10 mM stock) 1 0.1 mM
L-Glutamine (200 mM stock) 1 2 mM
• Wrap the Claycomb Medium bottle in aluminum foil, since the medium is extremely light sensitive.
• Supplemented Claycomb Medium is good for two weeks, at which time L-glutamine is replenished.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net