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pSMART BAC载体
Map图谱
CopyRight® v2.0 BAC Cloning Kits (pSMART® BAC and pEZ™ BAC)
Efficiently clone any size insert up to 200kb.
Create libraries from A/T- or G/C-rich genomes.
Clone gene clusters or operons.
High insert stability.
Inducible copy number.
Ordering
Resources
Cloning vector pSMART BAC v2.0, complete sequence
GenBank: EU101022.1
1 ccaccacagt gggatccgaa gcttggaatt cacgtgactt gaagtcgcgg ccgcactgac 61 cctatagtga gtcgtattaa tttaaatcat accaacatgg tcaaataaaa cgaaaggctc 121 agtcgaaaga ctgggccttt cgttttaatc tgatcggcac gtaagaggtt ccaactttca 181 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc 241 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg 301 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac 361 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta 421 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat ttcgtatggc 481 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca 541 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt 601 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa 661 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt 721 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata 781 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg 841 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca 901 gggcggggcg taacctaggt gacagaagtc aaaagcctcc ggtcggaggc ttttgacttt 961 ctgctagatc ggccgcatcg aatataactt cgtataatgt atgctatacg aagttattag 1021 cgatgagctc ggacttccat tgttcattcc acggacaaaa acagagaaag gaaacgacag 1081 aggccaaaaa gctcgctttc agcacctgtc gtttcctttc ttttcagagg gtattttaaa 1141 taaaaacatt aagttatgac gaagaagaac ggaaacgcct taaaccggaa aattttcata 1201 aatagcgaaa acccgcgagg tcgccgcccc gtaacctgtc ggatcaccgg aaaggacccg 1261 taaagtgata atgattatca tctacatatc acaacgtgcg tggaggccat caaaccacgt 1321 caaataatca attatgacgc aggtatcgta ttaattgatc tgcatcaact taacgtaaaa 1381 gcaacttcag acaatacaaa tcagcgacac tgaatacggg gcaacctcat gtccgagctc 1441 gcgagctcgt cgacagcgac acacttgcat cggatgcagc ccggttaacg tgccggcacg 1501 gcctgggtaa ccaggtattt tgtccacata accgtgcgca aaatgttgtg gataagcagg 1561 acacagcagc aatccacagc aggcatacaa ccgcacaccg aggttactcc gttctacagg 1621 ttacgacgac catgtcaata cttgcccttg acaggcattg atggaatcgt agtctcacgc 1681 tgatagtctg atcgacaata caagtgggac cgtggtccca gaccgataat cagaccgaca 1741 acacgagtgg gatcgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg 1801 gtcccagact aataatcaga ccgacgatac gagtgggacc gtggttccag actaataatc 1861 agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga cgatacgagt 1921 gggaccatgg tcccagacta ataatcagac cgacgatacg agtgggaccg tggtcccagt 1981 ctgattatca gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac 2041 gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt 2101 ggtcccagtc tgattatcag accgacgata caagtggaac agtgggccca gagagaatat 2161 tcaggccagt tatgctttct ggcctgtaac aaaggacatt aagtaaagac agataaacgt 2221 agactaaaac gtggtcgcat cagggtgctg gcttttcaag ttccttaaga atggcctcaa 2281 ttttctctat acactcagtt ggaacacgag acctgtccag gttaagcacc attttatcgc 2341 ccttatacaa tactgtcgct ccaggagcaa actgatgtcg tgagcttaaa ctagttcttg 2401 atgcagatga cgttttaagc acagaagtta aaagagtgat aacttcttca gcttcaaata 2461 tcaccccagc ttttttctgc tcatgaaggt tagatgcctg ctgcttaagt aattcctctt 2521 tatctgtaaa ggctttttga agtgcatcac ctgaccgggc agatagttca ccggggtgag 2581 aaaaaagagc aacaactgat ttaggcaatt tggcggtgtt gatacagcgg gtaataatct 2641 tacgtgaaat attttccgca tcagccagcg cagaaatatt tccagcaaat tcattctgca 2701 atcggcttgc ataacgctga ccacgttcat aagcacttgt tgggcgataa tcgttaccca 2761 atctggataa tgcagccatc tgctcatcat ccagctcgcc aaccagaaca cgataatcac 2821 tttcggtaag tgcagcagct ttacgacggc gactcccatc ggcaatttct atgacaccag 2881 atactcttcg accgaacgcc ggtgtctgtt gaccagtcag tagaaaagaa gggatgagat 2941 catccagtgc gtcctcagta agcagctcct ggtcacgttc attacctgac catacccgag 3001 aggtcttctc aacactatca ccccggagca cttcaagagt aaacttcaca tcccgaccac 3061 atacaggcaa agtaatggca ttaccgcgag ccattactcc tacgcgcgca attaacgaat 3121 ccaccatcgg ggcagctggt gtcgataacg aagtatcttc aaccggttga gtattgagcg 3181 tatgttttgg aataacaggc gcacgcttca ttatctaatc tcccagcgtg gtttaatcag 3241 acgatcgaaa atttcattgc agacaggttc ccaaatagaa agagcatttc tccaggcacc 3301 agttgaagag cgttgatcaa tggcctgttc aaaaacagtt ctcatccgga tctgaccttt 3361 accaacttca tccgtttcac gtacaacatt ttttagaacc atgcttcccc aggcatcccg 3421 aatttgctcc tccatccacg gggactgaga gccattacta ttgctgtatt tggtaagcaa 3481 aatacgtaca tcaggctcga accctttaag atcaacgttc ttgagcagat cacgaagcat 3541 atcgaaaaac tgcagtgcgg aggtgtagtc aaacaactca gcaggcgtgg gaacaatcag 3601 cacatcagca gcacatacga cattaatcgt gccgataccc aggttaggcg cgctgtcaat 3661 aactatgaca tcatagtcat gagcaacagt ttcaatggcc agtcggagca tcaggtgtgg 3721 atcggtgggc agtttacctt catcaaattt gcccattaac tcagtttcaa tacggtgcag 3781 agccagacag gaaggaataa tgtcaagccc cggccagcaa gtgggcttta ttgcataagt 3841 gacatcgtcc ttttccccaa gatagaaagg caggagagtg tcttctgcat gaatatgaag 3901 atctggtacc catccgtgat acattgaggc tgttccctgg gggtcgttac cttccacgag 3961 caaaacacgt agccccttca gagccagatc ctgagcaaga tgaacagaaa ctgaggtttt 4021 gtaaacgcct cctttatggg cagcaacccc gatcaccggt ggaaatacgt cttcagcacg 4081 tcgcaatcgc gtaccaaaca catcacgcat atgattaatt tgttcaattg tataaccaac 4141 acgttgctca acccgtcctc gaatttccat atccgggtgc ggtagtcgcc ctgctttctc 4201 ggcatctctg atagcctgag aagaaacccc aactaaatcc gctgcttcac ctattctcca 4261 gcgccgggtt attttcctcg cttccgggct gtcatcatta aactgtgcaa tggcgatagc 4321 cttcgtcatt tcatgaccag cgtttatgca ctggttaagt gtttccatga gtttcattct 4381 gaacatcctt taatcattgc tttgcgtttt tttattaaat cttgcaattt actgcaaagc 4441 aacaacaaaa tcgcaaagtc atcaaaaaac cgcaaagttg tttaaaataa gagcaacact 4501 acaaaaggag ataagaagag cacatacctc agtcacttat tatcactagc gctcgccgca 4561 gccgtgtaac cgagcatagc gagcgaactg gcgaggaagc aaagaagaac tgttctgtca 4621 gatagctctt acgctcagcg caagaagaaa tatccaccgt gggaaaaact ccaggtagag 4681 gtacacacgc ggatagccaa ttcagagtaa taaactgtga taatcaaccc tcatcaatga 4741 tgacgaacta acccccgata tcaggtcaca tgacgaaggg aaagagaagg aaatcaactg 4801 tgacaaactg ccctcaaatt tggcttcctt aaaaattaca gttcaaaaag tatgagaaaa 4861 tccatgcagg ctgaaggaaa cagcaaaact gtgacaaatt accctcagta ggtcagaaca 4921 aatgtgacga accaccctca aatctgtgac agataaccct cagactatcc tgtcgtcatg 4981 gaagtgatat cgcggaagga aaatacgata tgagtcgtct ggcggccttt ctttttctca 5041 atgtatgaga ggcgcattgg agttctgctg ttgatctcat taacacagac ctgcaggaag 5101 cggcggcgga agtcaggcat acgctggtaa ctttgaggca gctggtaacg ctctatgatc 5161 cagtcgattt tcagagagac gatgcctgag ccatccggct tacgatactg acacagggat 5221 tcgtataaac gcatggcata cggattggtg atttcttttg tttcactaag ccgaaactgc 5281 gtaaaccggt tctgtaaccc gataaagaag ggaatgagat atgggttgat atgtacactg 5341 taaagccctc tggatggact gtgcgcacgt ttgataaacc aaggaaaaga ttcatagcct 5401 ttttcatcgc cggcatcctc ttcagggcga taaaaaacca cttccttccc cgcgaaactc 5461 ttcaatgcct gccgtatatc cttactggct tccgcagagg tcaatccgaa tatttcagca 5521 tatttagcaa catggatctc gcagataccg tcatgttcct gtagggtgcc atcagatttt 5581 ctgatctggt caacgaacag atacagcata cgtttttgat cccgggagag actatatgcc 5641 gcctcagtga ggtcgtttga ctggacgatt cgcgggctat ttttacgttt cttgtgattg 5701 ataaccgctg tttccgccat gacagatcca tgtgaagtgt gacaagtttt tagattgtca 5761 cactaaataa aaaagagtca ataagcaggg ataactttgt gaaaaaacag cttcttctga 5821 gggcaatttg tcacagggtt aagggcaatt tgtcacagac aggactgtca tttgagggtg 5881 atttgtcaca ctgaaagggc aatttgtcac aacaccttct ctagaaccag catggataaa 5941 ggcctacaag gcgctctaaa aaagaagatc taaaaactat aaaaaaaata attataaaaa 6001 tatccccgtg gataagtgga taaccccaag ggaagttttt tcaggcatcg tgtgtaagca 6061 gaatatataa gtgctgttcc ctggtgcttc ctcgctcact cgaccgggag ggttcgagaa 6121 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa 6181 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa 6241 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc 6301 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct 6361 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt 6421 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct 6481 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg 6541 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt 6601 tccgcgaggt atccacaacg ccggcggccg gccgcggtgt ctcgcacacg gcttcgacgg 6661 cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag 6721 ccgagggctt cgccctgtcg ctcgactgcg gcgagcacta ctggctgtaa aaggacagac 6781 cacatcatgg ttctgtgttc attaggttgt tctgtccatt gctgacataa tccgctccac 6841 ttcaacgtaa caccgcacga agatttctat tgttcctgaa ggcatattca aatcgttttc 6901 gttaccgctt gcaggcatca tgacagaaca ctacttccta taaacgctac acaggctcct 6961 gagattaata atgcggatct ctacgataat gggagatttt cccgactgtt tcgttcgctt 7021 ctcagtggat aacagccagc ttctctgttt aacagacaaa aacagcatat ccactcagtt 7081 ccacatttcc atataaaggc caaggcattt attctcagga taattgtttc agcatcgcaa 7141 ccgcatcaga ctccggcatc gcaaactgca cccggtgccg ggcagccaca tccagcgcaa 7201 aaaccttcgt gtagacttcc gttgaactga tggacttatg tcccatcagg ctttgcagaa 7261 ctttcagcgg tataccggca tacagcatgt gcatcgcata ggaatggcgg aacgtatgtg 7321 gtgtgaccgg aacagagaac gtcacaccgt cagcagcagc ggcggcaacc gcctccccaa 7381 tccaggtcct gaccgttctg tccgtcactt cccagatccg cgctttctct gtccttcctg 7441 tgcgacggtt acgccgctcc atgagcttat cgggtccact cgcgaacgca gaaaggccca 7501 cccgaaggtg agccagtgtg attacatttg cggcctaact gtggccagtc cagttacgct 7561 ggagtcacta gtatttaggt gacactatag aagcggccgc gacaacttgt ccacgtggaa 7621 ttctaagctt aggatcccaa tgtgatgg //
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