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pEZ BAC载体质粒-BioVector NTCC保藏中心

  • 价　　格：¥9820
  • 货　　号：pEZ BAC
  • 产　　地：北京
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BioVector NTCC典型培养物保藏中心
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pEZ BAC载体

Map图谱


CopyRight® v2.0 BAC Cloning Kits (pSMART® BAC and pEZ™ BAC)

  • Efficiently clone any size insert up to 200kb.

  • Create libraries from A/T- or G/C-rich genomes.

  • Clone gene clusters or operons.

  • High insert stability.

  • Inducible copy number.

( more info)

  • Ordering

  • Resources

Product DescriptionSizeCat. No.PriceQuantityBUY
BAC-Optimized Replicator v2.0 Electrocompetent Cells
12 rxns (DUOs)60210-1$176.00


24 rxns (DUOs)60210-2$308.00
CopyRight v2.0 BAC Cloning Kit, pSMART BAC BamHI w/BAC-Optimized Replicator v2.0 Electrocompetent Cells
10 rxns42030-1$504.00


20 rxns42030-2$899.00
CopyRight v2.0 BAC Cloning Kit, pSMART BAC HindIII w/BAC-Optimized Replicator v2.0 Electrocompetent Cells
10 rxns42032-1$504.00


20 rxns42032-2$899.00
CopyRight v2.0 pEZ BAC Blunt Cloning Kit w/Electrocompetent Cells
10 rxns42009-1$504.00


20 rxns42009-2$899.00
CopyRight v2.0 pEZ BAC Blunt Cloning Kit without cells
10 rxns42016-1$389.00
ORDER INFORMATION

Each CopyRight® v2.0 BAC Cloning Kit contains: pre-cut vector (pSMART BAC with a choice of BamHI or HindIII sites), CopyRight DNA Ligase, CopyRight 5X Ligation Buffer, Positive Control Insert DNA, Sequencing Primers, BAC-Optimized Replicator v2.0 Electrocompetent Cells in DUO packaging (2 transformations per tube), Positive Control Insert DNA, Recovery Medium, Arabinose Induction Solution, and complete protocols. Each CopyRight pEZ BAC Cloning Kit contains: pre-cut CopyRight vector (pEZ BAC Blunt), CloneSmart DNA Ligase, CloneDirect™ 10X Ligation Buffer (includes ATP), Positive Control Insert DNA, Replicator Induction Solution, Sequencing Primers, and complete protocols. Kits with cells also include BAC-Optimized Replicator v2.0 Electrocompetent Cells (>2 × 1010 cfu/µg DNA) packaged in two transformations per tube (DUOs), Positive Control Plasmid, and Recovery Medium.

Please note that the following vectors have been discontinued: pSMART BAC EcoRI (catalog numbers 42031-1, 42031-2) and pEZ BAC BamHI (catalog numbers 42007-1, 42007-2 and 42012-1).”

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Cloning vector pEZ BAC, complete sequence


AAGGAATATTCAGCAATTTGCCCGTGCCGAAGAAAGGCCCACCCGTGAAG

GTGAGCCAGTGAGTTGATTGCTACGTAAATAACTTCGTATAGCATACATT

ATACGAAGTTATGGACTAGGCGCGCCAGAAGAGAGAAAGAAGGAAAGCGG

CCGCACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAA

TGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTC

CGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGA

AACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGAGACTATAGAAT

ACTCAAGCTTGCATGCGATACGTATCGTTAACGATGGATCCGACGCACGT

GCGAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTT

ACAACGTCGTGACTGGGAAAACCCTGGCGTCACCCAACTTAATCGCCTTG

CAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACC

GATCGCCCTTCCCAACAGTTGCGCAGCTGAATGGCGAATGGCGCCTGATG

CGGTATTTTCTCCTTACGGCGGCCGCTTGACATAACTTCGTATAGCATAC

ATTATACGAAGTTATGTTTAAACATTAGCAGAAAGTCAAAGGCCTCCGGT

CGGAGGCTTTTGACTAAAACTTCCCTTGGGGTTATCATTGGGCCGAGACC

GCCTGAAGAGGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGG

AAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTC

TTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC

GGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGG

TCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATA

ATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGT

CAAATAATCAATTATGACGCAGGTATCGTATTAATTGATCTGCATCAACT

TAACGTAAAAGCAACTTCAGACAATACAAATCAGCGACACTGAATACGGG

GCAACCTCATGTCGCCTGAAGAGTGAGACCGGCCCTGATCGGCACGTAAG

AGGCTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTT

TTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAA

ATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACA

TTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTC

AGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAG

TTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCC

GGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTG

TTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCG

CTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTC

GCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGT

TTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACC

AGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTT

CACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGG

CGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATG

CTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAA

ATGTAATCACCTGGCTCACCTTCGGGTGGGCCTTTCACACTTGCATCGGA

TGCAGCCCGGTGAACGTGCCGGCACGGCCTGGGTAACCAGGTATTTTGTC

CACATAACCGTGCGCAAAATGTTGTGGATAAGCAGGACACAGCAGCAATC

CACAGCAGGCATACAACCGCACACCGAGGTTACTCCGTTCTACAGGTTAC

GACGACATGTCAATACTTGCCCTTGACAGGCATTGATGGAATCGTAGTCT

CACGCTGATAGTCTGATCGACAATACAAGTGGGACCGTGGTCCCAGACCG

ATAATCAGACCGACAACACGAGTGGGATCGTGGTCCCAGACTAATAATCA

GACCGACGATACGAGTGGGACCGTGGTCCCAGACTAATAATCAGACCGAC

GATACGAGTGGGACCGTGGTTCCAGACTAATAATCAGACCGACGATACGA

GTGGGACCGTGGTCCCAGACTAATAATCAGACCGACGATACGAGTGGGAC

CATGGTCCCAGACTAATAATCAGACCGACGATACGAGTGGGACCGTGGTC

CCAGTCTGATTATCAGACCGACGATACGAGTGGGACCGTGGTCCCAGACT

AATAATCAGACCGACGATACGAGTGGGACCGTGGTCCCAGACTAATAATC

AGACCGACGATACGAGTGGGACCGTGGTCCCAGTCTGATTATCAGACCGA

CGATACAAGTGGAACAGTGGGCCCAGAGAGAATATTCAGGCCAGTTATGC

TTTCTGGCCTGTAACAAAGGACATTAAGTAAAGACAGATAAACGTAGACT

AAAACGTGGTCGCATCAGGGTGCTGGCTTTTCAAGTTCCTTAAGAATGGC

CTCAATTTTCTCTATACACTCAGTTGGAACACGAGACCTGTCCAGGTTAA

GCACCATTTTATCGCCCTTATACAATACTGTCGCTCCAGGAGCAAACTGA

TGTCGTGAGCTTAAACTAGTTCTTGATGCAGATGACGTTTTAAGCACAGA

AGTTAAAAGAGTGATAACTTCTTCAGCTTCAAATATCACCCCAGCTTTTT

TCTGCTCATGAAGGTTAGATGCCTGCTGCTTAAGTAATTCCTCTTTATCT

GTAAAGGCTTTTTGAAGTGCATCACCTGACCGGGCAGATAGTTCACCGGG

GTGAGAAAAAAGAGCAACAACTGATTTAGGCAATTTGGCGGTGTTGATAC

AGCGGGTAATAATCTTACGTGAAATATTTTCCGCATCAGCCAGCGCAGAA

ATATTTCCAGCAAATTCATTCTGCAATCGGCTTGCATAACGCTGACCACG

TTCATAAGCACTTGTTGGGCGATAATCGTTACCCAATCTGGATAATGCAG

CCATCTGCTCATCATCCAGCTCGCCAACCAGAACACGATAATCACTTTCG

GTAAGTGCAGCAGCTTTACGACGGCGACTCCCATCGGCAATTTCTATGAC

ACCAGATACTCTTCGACCGAACGCCGGTGTCTGTTGACCAGTCAGTAGAA

AAGAAGGGATGAGATCATCCAGTGCGTCCTCAGTAAGCAGCTCCTGGTCA

CGTTCATTACCTGACCATACCCGAGAGGTCTTCTCAACACTATCACCCCG

GAGCACTTCAAGAGTAAACTTCACATCCCGACCACATACAGGCAAAGTAA

TGGCATTACCGCGAGCCATTACTCCTACGCGCGCAATTAACGAATCCACC

ATCGGGGCAGCTGGTGTCGATAACGAAGTATCTTCAACCGGTTGAGTATT

GAGCGTATGTTTTGGAATAACAGGCGCACGCTTCATTATCTAATCTCCCA

GCGTGGTTTAATCAGACGATCGAAAATTTCATTGCAGACAGGTTCCCAAA

TAGAAAGAGCATTTCTCCAGGCACCAGTTGAAGAGCGTTGATCAATGGCC

TGTTCAAAAACAGTTCTCATCCGGATCTGACCTTTACCAACTTCATCCGT

TTCACGTACAACATTTTTTAGAACCATGCTTCCCCAGGCATCCCGAATTT

GCTCCTCCATCCACGGGGACTGAGAGCCATTACTATTGCTGTATTTGGTA

AGCAAAATACGTACATCAGGCTCGAACCCTTTAAGATCAACGTTCTTGAG

CAGATCACGAAGCATATCGAAAAACTGCAGTGCGGAGGTGTAGTCAAACA

ACTCAGCAGGCGTGGGAACAATCAGCACATCAGCAGCACATACGACATTA

ATCGTGCCGATACCCAGGTTAGGCGCGCTGTCAATAACTATGACATCATA

GTCATGAGCAACAGTTTCAATGGCCAGTCGGAGCATCAGGTGTGGATCGG

TGGGCAGTTTACCTTCATCAAATTTGCCCATTAACTCAGTTTCAATACGG

TGCAGAGCCAGACAGGAAGGAATAATGTCAAGCCCCGGCCAGCAAGTGGG

CTTTATTGCATAAGTGACATCGTCCTTTTCCCCAAGATAGAAAGGCAGGA

GAGTGTCTTCTGCATGAATATGAAGATCTGGTACCCATCCGTGATACATT

GAGGCTGTTCCCTGGGGGTCGTTACCTTCCACGAGCAAAACACGTAGCCC

CTTCAGAGCCAGATCCTGAGCAAGATGAACAGAAACTGAGGTTTTGTAAA

CGCCACCTTTATGGGCAGCAACCCCGATCACCGGTGGAAATACGTCTTCA

GCACGTCGCAATCGCGTACCAAACACATCACGCATATGATTAATTTGTTC

AATTGTATAACCAACACGTTGCTCAACCCGTCCTCGAATTTCCATATCCG

GGTGCGGTAGTCGCCCTGCTTTCTCGGCATCTCTGATAGCCTGAGAAGAA

ACCCCAACTAAATCCGCTGCTTCACCTATTCTCCAGCGCCGGGTTATTTT

CCTCGCTTCCGGGCTGTCATCATTAAACTGTGCAATGGCGATAGCCTTCG

TCATTTCATGACCAGCGTTTATGCACTGGTTAAGTGTTTCCATGAGTTTC

ATTCTGAACATCCTTTAATCATTGCTTTGCGTTTTTTTATTAAATCTTGC

AATTTACTGCAAAGCAACAACAAAATCGCAAAGTCATCAAAAAACCGCAA

AGTTGTTTAAAATAAGAGCAACACTACAAAAGGAGATAAGAAGAGCACAT

ACCTCAGTCACTTATTATCACTAGCGCTCGCCGCAGCCGTGTAACCGAGC

ATAGCGAGCGAACTGGCGAGGAAGCAAAGAAGAACTGTTCTGTCAGATAG

CTCTTACGCTCAGCGCAAGAAGAAATATCCACCGTGGGAAAAACTCCAGG

TAGAGGTACACACGCGGATAGCCAATTCAGAGTAATAAACTGTGATAATC

AACCCTCATCAATGATGACGAACTAACCCCCGATATCAAGTCACATGACG

AAGGGAAAGAGAAGGAAATCAACTGTGACAAACTGCCCTCAAATTTGGCT

TCCTTAAAAATTACAGTTCAAAAAGTATGAGAAAATCCATGCAGGCTGAA

GGAAACAGCAAAACTGTGACAAATTACCCTCAGTAGGTCAGAACAAATGT

GACGAACCACCCTCAAATCTGTGACAGATAACCCTCAGACTATCCTGTCG

TCATGGAAGTGATATCGCGGAAGGAAAATACGATATGAGTCGTCTGGCGG

CCTTTCTTTTTCTCAATGTATGAGAGGCGCATTGGAGTTCTGCTGTTGAT

CTCATTAACACAGACCTGCAGGAAGCGGCGGCGGAAGTCAGGCATACGCT

GGTAACTTTGAGGCAGCTGGTAACGCTCTATGATCCAGTCGATTTTCAGA

GAGACGATGCCTGAGCCATCCGGCTTACGATACTGACACAGGGATTCGTA

TAAACGCATGGCATACGGATTGGTGATTTCTTTTGTTTCACTAAGCCGAA

ACTGCGTAAACCGGTTCTGTAACCCGATAAAGAAGGGAATGAGATATGGG

TTGATATGTACACTGTAAAGCCCTCTGGATGGACTGTGCGCACGTTTGAT

AAACCAAGGAAAAGATTCATAGCCTTTTTCATCGCCGGCATCCTCTTCAG

GGCGATAAAAAACCACTTCCTTCCCCGCGAAACTCTTCAATGCCTGCCGT

ATATCCTTACTGGCTTCCGCAGAGGTCAATCCGAATATTTCAGCATATTT

AGCAACATGGATCTCGCAGATACCGTCATGTTCCTGTAGGGTGCCATCAG

ATTTTCTGATCTGGTCAACGAACAGATACAGCATACGTTTTTGATCCCGG

GAGAGACTATATGCCGCCTCAGTGAGGTCGTTTGACTGGACGATTCGCGG

GCTATTTTTACGTTTCTTGTGATTGATAACCGCTGTTTCCGCCATGACAG

ATCCATGTGAAGTGTGACAAGTTTTTAGATTGTCACACTAAATAAAAAAG

AGTCAATAAGCAGGGATAACTTTGTGAAAAAACAGCTTCTTCTGAGGGCA

ATTTGTCACAGGGTTAAGGGCAATTTGTCACAGACAGGACTGTCATTTGA

GGGTGATTTGTCACACTGAAAGGGCAATTTGTCACAACACCTTCTCTAGA

ACCAGCATGGATAAAGGCCTACAAGGCGCTCTAAAAAAGAAGATCTAAAA

ACTATAAAAAAAATAATTATAAAAATATCCCCGTGGATAAGTGGATAACC

CCAAGGGAAGTTTTTTCAGGCATCGTGTGTAAGCAGAATATATAAGTGCT

GTTCCCTGGTGCTTCCTCGCTCACTCGACCGGGAGGGTTCGAGAAGGGGG

GGCACCCCCCTTCGGCGTGCGCGGTCACGCGCACAGGGCGCAGCCCTGGT

TAAAAACAAGGTTTATAAATATTGGTTTAAAAGCAGGTTAAAAGACAGGT

TAGCGGTGGCCGAAAAACGGGCGGAAACCCTTGCAAATGCTGGATTTTCT

GCCTGTGGACAGCCCCTCAAATGTCAATAGGTGCGCCCCTCATCTGTCAG

CACTCTGCCCCTCAAGTGTCAAGGATCGCGCCCCTCATCTGTCAGTAGTC

GCGCCCCTCAAGTGTCAATACCGCAGGGCACTTATCCCCAGGCTTGTCCA

CATCATCTGTGGGAAACTCGCGTAAAATCAGGCGTTTTCGCCGATTTGCG

AGGCTGGCCAGCTCCACGTCGCCGGCCGAAATCGAGCCTGCCCCTCATCT

GTCAACGCCGCGCCGGGTGAGTCGGCCCCTCAAGTGTCAACGTCCGCCCC

TCATCTGTCAGTGAGGGCCAAGTTTTCCGCGAGGTATCCACAACGCCGGC

GGCCGGCCGCGGTGTCTCGCACACGGCTTCGACGGCGTTTCTGGCGCGTT

TGCAGGGCCATAGACGGCCGCCAGCCCAGCGGCGAGGGCAACCAGCCCGG

TGAGCGTCGGA


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