pAc-sgRNA-Cas9昆虫细胞基因编辑CRISPR/Cas9载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
- 价 格：¥9820
- 货 号：pAc-sgRNA-Cas9
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
pAc-sgRNA-Cas9
(BioVector-049330)
描述
Expresses sgRNA and Cas9-Puro in Drosophila S2 cells
- Vector backbonepAc-STABLE1-Puro(Search Vector Database)
- Backbone manufacturerDr. James Sutherland
Backbone size w/o insert (bp)6600
Total vector size (bp)10658
- Vector typeInsect Expression, CRISPR
- Selectable markersPuromycin
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)DH5alpha
- Copy numberHigh Copy
GENE/INSERT 1
- Gene/Insert nameCas9
- Alt nameCRISPR associated 9
- Alt namehspCas9
- SpeciesSynthetic; Streptococcus pyogenes
- Insert Size (bp)4278
- MutationHuman codon optimised
PromoterActin-5c
Tags / Fusion Proteins
3xFLAG (N terminal on insert)
NLS (N terminal on insert)
NLS (C terminal on insert)
CLONING INFORMATION FOR GENE/INSERT 1
Cloning methodRestriction Enzyme
5′ cloning siteEcoRI (not destroyed)
3′ cloning siteHindIII (not destroyed)
5′ sequencing primergagttcttgtgctgtgtgga
3′ sequencing primertcgagacaaacggcgaaacc
GENE/INSERT 2
- Gene/Insert namedU6-sgRNA
- SpeciesD. melanogaster (fly), Synthetic
- Insert Size (bp)500
PromoterDrosophila U6
CLONING INFORMATION FOR GENE/INSERT 2
Cloning methodRestriction Enzyme
5′ cloning siteBglII (not destroyed)
3′ cloning siteBglII (not destroyed)
5′ sequencing primerAAAAAAGCACCGACTCGGTGC
3′ sequencing primerGTTCGACTTGCAGCCTGAAATACG
Map图谱：
Sequence序列：
>pAc-sgRNA-Cas9 sequence 10663 bps
agatctaaaaaagcaccgactcggtgccactttttcaagttgataacggactagccttattttaacttgc
tatttctagctctaaaacggaagagctcgctcttccgaagtattgaggaaaacatacctatataaatgat
caacatcaggaaagagcagttgagaattataagaattggcaaatggtcttaagaaccctctgcttaagat
tttcaaaatttcctttagaatcaaagtgtcctattgttcgtttgttgaaaacacagttcgatttattgac
tataataaattgatagttttaaatatagaggcacgactaagagagtttggttttgttttggttagggacc
aaaaaaagtatacataacgaataaaaaaggatttaagtgcaaatggtaaaaaagtgtcgagtttttcttg
agttgattgtgctgtaatgagactctgcattcggcatttgactcggcttttcctactcgtgccgtatttc
aggctgcaagtcgaacagatctcgctgcctgttatgtggcccacaaaccaagacacgttttatggccatt
aaagctggctgatcgtcgccaaacaccaaatacatAatGaatatgtACacattcgagaaagaagcgatca
aagaagcgtcttcgggcGgagtaggagaatgcggaggagaaggagaacgagctgatctagtatctctcca
caatccaatgccaactgaccaactggccatattcggagcaatttgaagccaatttccatcgcctggcgat
cgctccattcttggctatatgtttttcaccgttAcccggggccattttcaaagactcgtcggCaagataa
gattgtgtcactcgctgtctctcttcatttgtcgaagaatgctgaggaatttcgcgatgacgtcggcgag
tattttgaagaatgagaataatttgtatttatacgaaaatcagttagtggaattttctacaaaaacatgt
tatctatagataattttgttgcaaaatatgttgactatgacaaagattgtatgtatatacctttaatgta
ttctcattttcttatgtatttataatggcaatgatgatactgatgatattttaagatgatgccagaccac
aggctgatttctgcgtcttttgccgaacgcagtgcatgtgcggttgttgttttttggaatagtttcaatt
ttcggactgtccgctttgatttcagtttcttggcttattcaaaaagcaaagtaaagccaaaaaagcgaga
tggcaataccaaatgcggcaaaacggtagtggaaggaaaggggtgcggggcagcggaaggaagggtgggg
cggggcgtggcggggtctgtggctgggcgcgacgtcaccgacgttggagccactcctttgaccatgtgtg
cgtgtgtgtattattcgtgtctcgccactcgccggttgtttttttctttttatctcgctctctctagcgc
catctcgtacgcatgctcaacgcaccgcatgttgccgtgtcctttatgcgtcattttggctcgaaatagg
caattatttaaacaaagattagtcaacgaaaacgctaaaataaataagtctacaatatggttacttattg
ccatgtgtgtgcagccaacgatagcaacaaaagcaacaacacagtggctttccctctttcactttttgtt
tgcaagcgcgtgcgagcaagacggcacgaccggcaaacgcaattacgctgacaaagagcagacgaagttt
tggccgaaaaacatcaaggcgcctgatacgaatgcatttgcaataacaattgcgatatttaatattgttt
atgaagctgtttgacttcaaaacacacaaaaaaaaaaataaaacaaattatttgaaagagaattaggaat
cggacagcttatcgttacgggctaacagcacaccgagacgaaatagcttacctgacgtcacagcctctgg
aagaactgccgccaagcagagagagagagaaaaagagggagagcagcttagaccgcatgtgcttgtgtgt
gaggcgtctctctcttcgtctcctgtttgcgcaaacgcatagactgcactgagaaaatcgattacctatt
ttttatgaatgaatatttgcactattactattcaaaactattaagatagcaatcacattcaatagccaaa
tactataccacctgagcgatgcaacgaaatgatcaatttgagcaaaaatgctgcatatttaggacggcat
cattatagaaatgcttcttgctgtgtacttttctctcgtctggcagctgtttcgccgttattgttaaaac
cggcttaagttaggtgtgttttctacgactagtgatgcccctactagaagatgtgtgttgcacaaatgtc
cctgaataaccaatttgaagtgcagatagcagtaaacgtaagctaatatgaatattatttaactgtaatg
ttttaatatcgctggacattactaataaacccactataaacacatgtacatatgtatgttttggcataca
atgagtagttggggaaaaaatgtgtaaaagcaccgtgaccatcacagcataaagataaccagctgaagta
tcgaatatgagtaacccccaaattgaatcacatgccgcaactgataggacccatggaagtacactcttca
tggcgatatacaagacacacacaagcacgaacacccagttgcggaggaaattctccgtaaatgaaaaccc
aatcggcgaacaattcatacccatatatggtaaaagttttgaacgcgacttgagagcggagagcattgcg
gctgataaggttttagcgctaagcgggctttataaaacgggctgcgggaccagttttcatatcactaccg
tttgagttcttgtgctgtgtggatactcctcccgacacaaagccgctccatcagccagcagtcgtctaat
ccagagaccccggatcggggtacctactagtccagtgtggtggaattcatggactataaggaccacgacg
gagactacaaggatcatgatattgattacaaagacgatgacgataagatggccccaaagaagaagcggaa
ggtcggtatccacggagtcccagcagccgacaagaagtacagcatcggcctggacatcggcaccaactct
gtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccg
accggcacagcatcaagaagaacctgatcggagccctgctgttcgacagcggcgaaacagccgaggccac
ccggctgaagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagagatc
ttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtccttcctggtggaag
aggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaagta
ccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcggctgatctat
ctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaaca
gcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaa
cgccagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctg
atcgcccagctgcccggcgagaagaagaatggcctgttcggaaacctgattgccctgagcctgggcctga
cccccaacttcaagagcaacttcgacctggccgaggatgccaaactgcagctgagcaaggacacctacga
cgacgacctggacaacctgctggcccagatcggcgaccagtacgccgacctgtttctggccgccaagaac
ctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccgagatcaccaaggcccccctgagcg
cctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagca
gctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggctacattgacggc
ggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaac
tgctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatccccca
ccagatccacctgggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggac
aaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggccctctggccaggggaa
acagcagattcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaacttcgaggaagtggt
ggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaacgag
aaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagctgaccaaagtgaaat
acgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggccatcgtggacctgct
gttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagtgcttc
gactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctga
aaattatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgac
cctgacactgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgacgac
aaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagccggaagctgatcaacg
gcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcgccaacagaaa
cttcatgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaagcccaggtgtccggc
cagggcgatagcctgcacgagcacattgccaatctggccggcagccccgccattaagaagggcatcctgc
agacagtgaaggtggtggacgagctcgtgaaagtgatgggccggcacaagcccgagaacatcgtgatcga
aatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatcgaa
gagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagctgcagaacg
agaagctgtacctgtactacctgcagaatgggcgggatatgtacgtggaccaggaactggacatcaaccg
gctgtccgactacgatgtggaccatatcgtgcctcagagctttctgaaggacgactccatcgacaacaag
gtgctgaccagaagcgacaagaaccggggcaagagcgacaacgtgccctccgaagaggtcgtgaagaaga
tgaagaactactggcggcagctgctgaacgccaagctgattacccagagaaagttcgacaatctgaccaa
ggccgagagaggcggcctgagcgaactggataaggccggcttcatcaagagacagctggtggaaacccgg
cagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacgacgagaatgacaagc
tgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaaggatttccagtt
ttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgtcgtgggaacc
gccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgc
ggaagatgatcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacat
catgaactttttcaagaccgagattaccctggccaacggcgagatccggaagcggcctctgatcgagaca
aacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcggaaagtgctgagca
tgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaaagagtctatcctgcc
caagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaagtacggcggcttcgac
agccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagtccaagaaactgaaga
gtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatcgactttct
ggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgag
ctggaaaacggccggaagagaatgctggcctctgccggcgaactgcagaagggaaacgaactggccctgc
cctccaaatatgtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggataa
tgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatcgagcagatcagcgag
ttctccaagagagtgatcctggccgacgctaatctggacaaagtgctgtccgcctacaacaagcaccggg
ataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgc
cgccttcaagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaagaggtgctggacgcc
accctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcagctgggaggcgaca
aaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaagggatcaggcaagcttgagggcag
aggaagtcttctaacatgcggtgacgtggaggagaatcccggccctgctagcggtagcggcagcggtagc
atgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcg
ccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcac
cgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggc
gccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgc
gcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccg
gcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggc
agcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccg
cgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaagg
accgcgcacctggtgcatgacccgcaagcccggtgcctgactcgagggatccagacatgataagatacat
tgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgct
attgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgt
ttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctga
ttatgatcagtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcat
gactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctc
tgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcg
gaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggc
cattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatg
gccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtcca
ggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagccagcaaaaggc
caggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaa
aatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaa
gctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggg
aagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctg
ggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtcca
acccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgt
aggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatc
tgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg
ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcc
tttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgaga
ttatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatat
atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatt
tcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggc
cccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccag
ccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccg
ggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtg
gtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgat
cccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgc
agtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttt
tctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcc
cggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttc
ttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcaccc
aactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccg
caaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaag
catttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg
gttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacct
ataaaaataggcgtatcacgaggccctttcgtcttcaaggaattaattctatattctaaaaacacaaatg
atacttctaaaaaaaatcatgaatggcatcaactctgaatcaaatctttgcagatgcacctacttctcat
ttccactgtcacatcatttttcc
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