pUTgfp载体

• pUTgfp载体

• 
  

• 质粒图谱

• 

• 
  

• pUT-miniTn5 Cm

• 
  

• 图谱Map

• 

  

  

  

  
  

The mini-Tn5 are mobile elements able to transpose to the chromosome from a delivery plasmid, by means of the transposase activity encoded by the tnp* gene, which is present in cis in the plasmid but external to the mini-Tn5 element. pUT mini-Tn5 vectors can be used to insert any DNA fragment in the bacterial chromosome, by cloning it within the mini-Tn5 region. Due to the loss of the tnp* gene after insertion, minitransposons are stably integrated into the chromosome and inherited. The different pUTmini-Tn5 vectors can then be used for repeated insertion events and therefore multiple insertions in the same strain are only limited by the availability of distinct selection markers.

Advantages:

• Easy and stable insertion of heterologous DNA in the chromosome of the strain of interest, after few simple genetic manipulations.

• Wide range of inserted fragment sizes.

• Possibility of multiple insertions in the same strain.

• Easy modification of the cloning vector.

• Use of cleavage sites of the rare cutters NotI and SfiI.

• Wide range of Gram negative recipient strains.

Some of the most common applications of pUTmini-Tn5 vectors are:

• Cloning.

• Insertional mutagenesis.

• Promoter probing.

• Generation of conditional phenotypes and non-polar mutations.

• Analysis of transcriptional terminators.

• Environmental applications: tagging with marker genes for monitoring of released microorganisms and/or transcriptional regulation in the environment.

• 
  

• ---------------------

• pUT mini-Tn5 Cm kit

  • pUT mini-Tn5 Cm, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn Km kit

  • pUT mini-Tn5 Km, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2073), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn Sm/Sp kit

  • pUT mini-Tn5 Sm/Sp, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn Tc kit

  • pUT mini-Tn5 Tc, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

  PANC-1 mini-Tn5 package

  • pUT mini-Tn5 Km, 2 μg.

  • pUT mini-Tn5 Cm, 2 μg.

  • pUT mini-Tn5 Sm/Sp, 2 μg.

  • pUT mini-Tn5 Tc, 2 μg.

  
  

  PANC-1plus miniTn5 package

  • pUT mini-Tn5 Km, 2 μg.

  • pUT mini-Tn5 Cm, 2 μg.

  • pUT mini-Tn5 Sm/Sp, 2 μg.

  • pUT mini-Tn5 Tc, 2 μg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • E. coli DH5a(pRK2073), stab.

  • TSS solution, 1.5ml.

  
  

  pUT mini-Tn5Tel kit

  • pUT mini-Tn5Tel vector, 2 μg.

  • Potassium Tellurite, 100mg.

  • E. coli DH5a lpir, stab.

  • E. coli S17-1 lpir, stab.

  • E. coli DH5a(pRK2013), stab.

  • TSS solution, 1.5ml.

  
  

质粒载体菌株细胞株基因保藏中心-Biovector Science Lab,Inc.可提供数万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等服务。作为美国质粒载体菌株细胞株基因保藏中心分库及唯一办事处，Biovector Inc.可为您提供便捷一站式的产品进口服务，到货快捷，步骤简便。通过Google搜索"Biovector+您所需资源名称",您一定能找到所需的资源和技术服务。如有需要请联系：

Dr Xu,Senior Scientist
电邮Biovector@163.com
电话18901268599

订购合同下载请点击：Biovector质粒载体菌株细胞基因订购合同-Biovector Science Lab

^_^寻找您所需质粒载体菌株基因细胞株等资源的窍门：在Google，Baidu等搜索引擎中搜索“Biovector您所需的资源名称”结果即刻闪现，马上试试吧^_^

点击浏览器菜单“编辑”-->"查找"或者直接按“Ctrl+F”查找您所需资源

由于页面字数限制，尚有很多质粒载体菌株基因片段名称未列出，如查找不到您所需资源，请发邮件或电话查询。

Biovector,Inc质粒载体菌株细胞株基因保藏中心列表网址http://biovector.blog.163.com

原核表达载体

• 真核表达载体

• 哺乳动物表达载体

• 荧光定位表达载体

• 慢病毒/腺病毒/逆病毒..

• RNAi基因沉默载体

• 乳酸菌表达载体/乳酸杆..

• 枯草芽孢杆菌表达载体

• 酿酒/毕赤酵母表达载体..

• 工具酶质粒Taq,KO..

• GS筛选表达系统

• 疫苗表达载体/抗体表达

• 革兰氏阳性菌表达/金葡..

• 谷氨酸棒状杆菌表达载体

• 噬菌体展示系统/抗体表..

• 酵母单杂交/双杂交/三..

• BiFC双分子荧光载体..

• 昆虫/杆状病毒表达载体

• 转座子质粒载体/Pig..

• 基因重组/敲除载体/基..

• Gateway系统载体

• 植物表达载体/荧光定位..

• 农杆菌感受态细胞及菌株

• 信号通路报告载体

• 四环素诱导表达系统

• 真菌表达/黑曲霉/黄曲..

• 病毒包装系统

• 各种大肠杆菌及感受态细..

• Addgene质粒/A..

• 人鼠基因库cDNA,O..

• 细胞株

• 链霉菌表达/敲除/链球..

• 细胞永生化载体

• iPS干细胞重编程载体

• 多基因表达/双启动子载..

• 质粒构建技术服务

• 基因克隆技术服务

• 果蝇细胞表达载体

• 多宿主载体/假单胞菌表..

• 悬浮细胞大规模表达载体..

• 报告基因载体

• 逆转录病毒载体

• 腺病毒载体/腺相关病毒..

• LC3自噬质粒载体

• 线虫表达载体RNAi

• 哺乳动物双杂交系统

• 蜕皮激素诱导表达系统

• **质粒载体

• 原核荧光表达载体

• 温度敏感型表达载体

• 共表达载体

• Cre/LoxP载体C..

• 用户
• 内容
• 询问日期