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Plasmid backbones/Nomenclature载体骨架与命名

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Plasmid backbones/Nomenclature



Overview

BioBrick plasmid backbone names take the form pSB#X#. The letters pSB are an acronym for plasmid Synthetic Biology.

  • The first number denotes the origin of replication.

  • The letter X identifies the antibiotic resistance marker(s) present in the vector. Plasmid backbones with multiple resistance markers have multiple, successive letters.

  • The last number in the plasmid backbone name is a version number to differentiate between the various implementations of the pSB series of plasmid backbones.

When referring to both a BioBrick standard biological part and the plasmid backbone in which it is cloned, the convention is to use the form [plasmid backbone name]-[part number] like pSB4K5-T9003. To refer to BioBrick standard plasmid backbones to be used for construction of BioBrick parts, use the full plasmid backbone name and default cloned part. For example, pSB4A3-P1010, pSB1A10-P1010, pSB4K5-I52002 and pSB3T5-I52001 are all available construction plasmids from the Registry of Standard Biological Parts. However, for convenience, these construction plasmid names are often abbreviated to pSB4A3, pSB1A10, pSB4K5 and pSB3T5, respectively.

New plasmid-based vectors constructed from BBa_I51020 should be named pSB#X5-I52002 where the # is determined by the identity of the replication origin and the letter X is determined by the antibiotic resistance marker(s) present. To expand the BioBrick vector nomenclature, submit new vectors or vector parts to the Registry of Standard Biological Parts and then document any new annotation needed here. The BioBricks Foundation will lead the standards setting process should any revisions to the BioBrick plasmid backbone nomenclature beyond addition of new replication origins, antibiotic resistance markers and version numbers be needed.

Origin of replication

BioBrick plasmid backbone names take the form pSB#X#. The first number indicates the identity of the origin of replication. The number, corresponding replication origin, expected plasmid copy number and typical purpose of that origin are listed. To expand this list to include additional replication origins, obtain an account with the Registry of Standard Biological Parts and edit this page.

NumberReplication originCopy numberPurpose
1modified pMB1 derived from pUC19500-700Easy plasmid DNA purification
2F and P1 lytic derived from pSCANS-1-BNL1-2 inducible to high copyInducible copy number
3p15A derived from pMR10110-12Multi-plasmid engineered systems
4rep101, repA derived from pSC101~5Small cell to cell copy number variation
5derived from F plasmid1-2Improved plasmid stability
6pMB1 derived from pBR32215-20Multi-plasmid engineered systems
7broad host range origin
by Ichiro et al.
8pSC101TS
temperature sensitive origin, only propagates at 30C
9


Selection marker

BioBrick plasmid backbone names take the form pSB#X#. The letter X indicates the antibiotic to which the plasmid backbone confers resistance. The letter code and corresponding antibiotic resistance marker are listed. The absence of a letter indicates that no antibiotic resistance marker is present. Multiple resistance markers in a vector are indicated by successive codes in alphabetical order e.g., AK, StT, AC and AKT. To expand this list to include additional antibiotic resistance markers, obtain an account with the Registry of Standard Biological Parts and edit this page.

CodeAntibiotic
Aampicillin
Cchloramphenicol
Eerythromycin
Ggentamycin
Kkanamycin
Nneomycin
Nanalidixic acid
Rrifampicin
Sspectinomycin
Ststreptomycin
Ttetracycline
Tmtrimethoprim
Zzeocin

Version number

BioBrick vector names take the form pSB#X#. The second number indicates the BioBrick plasmid backbone version number. The version number, key features, purpose for which that version was designed, example vector and vector designer(s) are listed. To expand this list to assign new vector version numbers, obtain an account with the Registry of Standard Biological Parts and edit this page.

NumberKey featuresPurposeExampleDesigner
0absent or incomplete BioBrick cloning site
pSB2K0Brookhaven National Lab
1complete BioBrick cloning site (BCS)assembly of BioBrick partspSB4A1Reshma Shetty
25' terminator and BCStranscriptional insulation of plasmid backbone upstream of cloned BioBrick partpSB1A2Tom Knight
35' terminator and BCS and 3' terminatortranscriptional insulation of plasmid backbone downstream of cloned BioBrick partpSB1AC3Reshma Shetty & Tom Knight
4pSB2K3-derived plasmid backbone free of many restriction sitesGenome refactoringChan-Mol-Syst-Biol-2005pSB2K4Leon Chan
5constructed from BioBrick base vectorstandardized BioBrick vector designpSB4K5Reshma Shetty
6Reserved for some plasmid backbone versions that the Berkeley iGEM team has made


7BCS flanked by terminator BBa_B0015transcriptional insulation of cloned BioBrick partpSB1A7Karmella Haynes
8BCS followed by eukaryotic terminatorfor cloning of eukaryotic composite partspSB1AK8Monika Ciglic and the 2007 Slovenian iGEM team
9



10Screening plasmid v1.0characterization of single input, single output transcriptional devicespSB1A10Josh Michener & Jason Kelly
11Reserved for a version of Jason's measurement plasmid


12Reserved for a version of Jason's measurement plasmid


13Reserved for a version of Reshma's measurement plasmid


15-19Reserved for new lambda red compatible BioBrick backbones.For chromosomal integration of single gene and operon of BioBricks.
Erik Lundin
20-29Reserved for a version of Berkeley iGEM team's plasmids


30Expression Plasmid Backbone harbouring a LacI repressible T7 Promotor right in front of the BB prefix based on pSBXX3 backbones.
pSB1A30iGEM team Heidelberg 2014
50Expression Plasmid Backbone harbouring a LacI repressible T7 Promotor right in front of the BB prefix based on pSBXX5 backbones.
pSB1A50iGEM team Heidelberg 2014


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