PANC-1 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

PANC-1 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
PANC-1 cell line细胞株
Organism Homo sapiens, human
Tissue pancreas/duct
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease epithelioid carcinoma
Age 56 years
Gender male
Ethnicity Caucasian
Applications This cell line is a suitable transfection host.
Karyotype Chromosome studies indicate a modal number of 63 with 3 distinct marker chromosomes and a small ring chromosome. This is a hypertriploid human cell line. The modal chromosome number was 61, occurring in 32% of cells., However, cells with 63 chromosomes also occurred at a high frequency (22%). The rate of cells with higher ploidies was 8.5%.
Images

Clinical Data 56 years
Caucasian
male
Comments Growth is inhibited by 1 unit/mL L-asparaginase.
The cells will grow in soft agar.
Complete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation culture medium 95%; DMSO, 5%
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 11,13
D13S317: 11
D7S820: 8,10
D16S539: 11
vWA: 15
THO1: 7,8
Amelogenin: X
TPOX: 8,11
CSF1PO: 10,12
Isoenzymes G6PD, B
Population Doubling Time 52 hrs
Deposited As Homo sapiens
References Lieber M, et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975. PubMed: 1140870


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