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pSpCas9n(BB)-2A-Puro (PX462) V2.0质粒载体CRISPR/Cas9系统-BioVector NTCC保藏中心

  • 价  格:¥3920
  • 货  号:62987
  • 产  地:北京
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pSpCas9n(BB)-2A-Puro (PX462) V2.0
(BioVector-062987)

  • PURPOSE
    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)
  • DEPOSITING LAB
    Feng Zhang
  • PUBLICATION
    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.

BACKBONE

  • Vector backbone
    PX462
    (Search Vector Database)
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

SEQUENCE INFORMATION


GENE/INSERT

  • Gene/Insert name
    hSpCas9n-2A-Puro
  • Alt name
    SpCas9n-2A-Puro V2.0
  • Alt name
    Cas9n
  • Alt name
    PX462 V2.0
  • Species
    Synthetic
  • Insert Size (bp)
    6000
  • Mutation
    Cas9 D10A nickase mutant
  • Tags / Fusion Proteins

    • 3XFLAG (N terminal on insert)

    • 2A-Puro (C terminal on insert)










  • For your References section:

    Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. 10.1038/nprot.2013.143 PubMed 24157548


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