pSpCas9n(BB)-2A-Puro (PX462) V2.0质粒载体CRISPR/Cas9系统-BioVector NTCC保藏中心
- 价 格：¥3920
- 货 号：62987
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pSpCas9n(BB)-2A-Puro (PX462) V2.0
(BioVector-062987)
- PURPOSECas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)
- DEPOSITING LABFeng Zhang
- PUBLICATIONRan et al Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24.
BACKBONE
- Vector backbonePX462(Search Vector Database)
- Vector typeMammalian Expression, CRISPR
- Selectable markersPuromycin
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)Stbl3
- Copy numberUnknown
SEQUENCE INFORMATION
GENE/INSERT
- Gene/Insert namehSpCas9n-2A-Puro
- Alt nameSpCas9n-2A-Puro V2.0
- Alt nameCas9n
- Alt namePX462 V2.0
- SpeciesSynthetic
- Insert Size (bp)6000
- MutationCas9 D10A nickase mutant
Tags / Fusion Proteins
3XFLAG (N terminal on insert)
2A-Puro (C terminal on insert)
For your References section:
Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. 10.1038/nprot.2013.143 PubMed 24157548
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