出品公司: | Addgene |
---|---|
载体名称: | pPRIME-TET-GFP-FF3 |
质粒类型: | 慢病毒系统载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | TET |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8226bp |
5' 测序引物及序列: | EGFP-C Forward: CATGGTCCTGCTGGAGTTCGTG |
3' 测序引物及序列: | -- |
载体标签: | GFP |
载体抗性: | 氯霉素(Chloramphenicol) |
筛选标记: | -- |
备注: | -- |
产品目录号: | 11662 |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | 慢病毒 |
pPRIME-TET-GFP-FF3慢病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格:¥1920
- 货 号:BiovectorPRIME-TET-GFP-FF3
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pPRIME-TET-GFP-FF3 8226 bp DNA SYN DEFINITION pPRIME-TET-GFP-FF3 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8226 /organism="pPRIME-TET-GFP-FF3" /mol_type="other DNA" promoter 239..803 /label="CMV_immearly_promoter" misc_feature 318..605 /label="CAG_enhancer" misc_feature 772..792 /label="CMV_fwd_primer" misc_feature 820..976 /label="HIV-1_5_LTR" misc_feature 820..976 /label="truncHIV-1_3_LTR" misc_feature 1087..1131 /label="HIV-1_psi_pack" CDS 1523..2410 /label="ORF frame 2" misc_feature 1645..1878 /label="RRE" misc_feature 2409..2424 /label="cPPT" CDS 2663..3700 /label="ORF frame 2" misc_feature 2882..2901 /label="pCEP_fwd_primer" misc_feature 2884..2908 /label="LNCX_primer" CDS 2928..3647 /label="ORF frame 3" gene 2931..3644 /label="EGFP" /gene="EGFP" misc_feature complement(2973..2994) /label="EGFP_N_primer" misc_feature 3111..3140 /label="Y66 (EGFP)" misc_feature 3581..3602 /label="EGFP_C_primer" gene 4172..4831 /label="CAT/CamR" /gene="CAT/CamR" CDS 4172..4831 /label="ORF frame 2" misc_feature 5117..5704 /label="WPRE" CDS 5205..5759 /label="ORF frame 3" CDS 5218..6258 /label="ORF frame 1" misc_feature complement(5707..5723) /label="pBluescriptKS_primer" misc_feature 5895..5910 /label="cPPT" misc_feature 5895..5916 /label="U3PPT" misc_feature 6232..6412 /label="HIV-1_5_LTR" misc_feature 6232..6412 /label="truncHIV-1_3_LTR" gene complement(7233..8093) /label="Ampicillin" /gene="Ampicillin" CDS complement(7233..8093) /label="ORF frame 2" promoter complement(8135..8163) /label="AmpR_promoter" ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata gaccagatct gagcctggga gctctctggc 841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 961 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg 1021 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc 1081 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg 1141 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc 1201 cagggggaaa gaaaaaatat aaataaacat atagtatggg caagcaggga gctagaacga 1261 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag 1321 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca 1381 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag 1441 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat 1501 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa 1561 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca 1621 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg 1681 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg 1741 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca 1801 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct 1861 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc 1921 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac 1981 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga 2041 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc 2101 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat 2161 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag 2221 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc 2281 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg 2341 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc 2401 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca 2461 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt 2521 ttcgggttta ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga 2581 cctttcgtct tcattcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac 2641 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat 2701 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga 2761 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc 2821 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag 2881 cagagctcgt ttagtgaacc gtcagatcgc ctggaccggt cgccaccatg gtgagcaagg 2941 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 3001 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 3061 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 3121 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 3181 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 3241 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 3301 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 3361 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 3421 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 3481 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 3541 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 3601 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtagcgg ccgcaagcct 3661 tgttaagtgc tcgcttcggc agcacatata ctatgtttga atgaggcttc agtactttac 3721 agaatcgttg cctgcacatc ttggaaacac ttgctgggat tacttcttca ggttaaccca 3781 acagaaggct cgagcaacca gaattcaagg ggctacttta ggagcaatta tcttgtttac 3841 taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta 3901 taaattaaat cacttttttc aattggaaga ctaatgcgtt taaacacgcg gcgacgcgtt 3961 cgaccgaata aaacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc 4021 tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt 4081 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt 4141 gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac 4201 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca 4261 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa 4321 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca 4381 tccggaatta cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc 4441 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca 4501 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa 4561 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg 4621 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt 4681 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca 4741 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca 4801 gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta 4861 aacgcctggt tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgga 4921 tctcgaccgc gtttgggcgg tggctccctg ccacgcggct ccgaacagaa gctgatctcc 4981 gaagaggatc tgacatgtgt ttaaacctcg acttaattaa gtcgagggtc gacggtatcg 5041 ataagctcgc ttcacgagat catgtttaag ggttccggtt ccactaggta caattcgata 5101 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 5161 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 5221 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 5281 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 5341 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 5401 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 5461 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 5521 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 5581 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 5641 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 5701 cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc 5761 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg 5821 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat 5881 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga 5941 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac 6001 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta 6061 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac 6121 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac 6181 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc 6241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 6301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 6361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga 6421 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 6481 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 6541 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 6601 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 6661 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 6721 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 6781 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 6841 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 6901 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 6961 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 7021 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7081 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 7141 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 7201 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 7261 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 7321 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 7381 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 7441 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 7501 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 7561 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 7621 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 7681 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 7741 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 7801 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 7861 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 7921 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 7981 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 8041 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 8101 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 8161 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 8221 cctgac //
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