β2163 strain菌株菌种beta 2163 BioVector NTCC质粒载体菌种细胞基因保藏中心

β2163 strain菌株菌种beta 2163 BioVector NTCC质粒载体菌种细胞基因保藏中心
Strain β2163 is a MG1655 derivative hosting RP4-2-Tc::Mu in the glvB gene of its chromosome [9]. RP4-2-Tc::Mu contains the transfer functions of the conjugative plasmid RP4, together with a kanamycine resistance gene, and a Mu-prophage inserted in its tetracycline resistance gene. In addition, this strain contains the pir gene and erythromycine resistance gene integrated at the dap locus, so that it requires 0.3 mM diaminopimelate for growth. An EcoK restriction and modification mutant derivative of strain β2163 was constructed (strain MAC1306), in which the two genes hsdR hsdM were replaced by a phleomycin resistance (phleoR) gene. Like strain β2163, MAC1306 contains the Mu prophage [9]. We verified that Mu did not interfere with the recombination assay (not shown). The single mutant ΔhsdR::FRT derivative of β2163 was also constructed (strain MAC1308), to use as a donor strain in conjugation experiments when the plasmid had to be protected from EcoK restriction in the recipient strain.
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