出品公司: | Addgene |
---|---|
载体名称: | pRSV-rev |
质粒类型: | 慢病毒系统;包装载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | RSV U3 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4174bp |
5' 测序引物及序列: | pREP fwd: GCTCGATACAATAAACGCC |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素(Ampicillian) |
筛选标记: | -- |
备注: | 第三代慢病毒系统包装载体。 |
产品目录号: | 12253 |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | 慢病毒 |
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载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter.Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system.
载体序列
LOCUS pRSV-Rev 4174 bp DNA SYN DEFINITION pRSV-Rev ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4174 /organism="pRSV-Rev" /mol_type="other DNA" promoter 143..172 /label="lac_promoter" misc_feature 186..208 /label="M13_pUC_rev_primer" promoter 207..225 /label="M13_reverse_primer" misc_feature 579..597 /label="pREP_fwd_primer" misc_feature 638..662 /label="LNCX_primer" misc_feature 978..1007 /label="HIV_Rev_NES" promoter complement(1190..1206) /label="M13_forward20_primer" rep_origin 1549..1855 /label="f1_origin" misc_feature 2123..2145 /label="pGEX_3_primer" promoter 2304..2332 /label="AmpR_promoter" gene 2374..3234 /label="Ampicillin" /gene="Ampicillin" CDS 2374..3234 /label="ORF frame 1" rep_origin 3389..4008 /label="pBR322_origin" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcgatg 241 tacgggccag atatacgcgt atctgagggg actaggtgtg tttaggcgaa aagcggggct 301 tcggttgtac gcggttagga gtcccctcag gattagtagt ttcgcttttg catagggagg 361 gggaaatgta gtcttatgca atacacttgt agtcttgcaa catggtaacg atgagttagc 421 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt 481 acgatcgtgc cttattagga aggcaacaga caggtctgac atggattgga cgaaccactg 541 aattccgcat tgcagagata attgtattta agtgcctagc tcgatacaat aaacgccatt 601 tgaccattca ccacattggt gtgcacctcc aagctcgagc tcgtttagtg aaccgtcaga 661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca 721 gcctcccctc gaagctagtc gattaggcat ctcctatggc aggaagaagc ggagacagcg 781 acgaagacct cctcaaggca gtcagactca tcaagtttct ctatcaaagc aacccacctc 841 ccaatcccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac 901 agagacagat ccattcgatt agtgaacgga tccttagcac ttatctggga cgatctgcgg 961 agcctgtgcc tcttcagcta ccaccgcttg agagacttac tcttgattgt aacgaggatt 1021 gtggaacttc tgggacgcag ggggtgggaa gccctcaaat attggtggaa tctcctacaa 1081 tattggagtc aggagctaaa gaatagtgct gttagcttgc tcaatgccac agctatagca 1141 gtagctgagg ggacagatag ggttatagaa gtagtacaag aagcttggca ctggccgtcg 1201 ttttacatga tctgagcctg ggagatctct ggctaactag ggaacccact gcttaagcct 1261 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 1321 aactagagat caggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc 1381 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg 1441 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 1501 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg 1561 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 1621 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 1681 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 1741 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 1801 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 1861 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg 1921 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt 1981 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc 2041 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 2101 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 2161 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 2221 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 2281 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 2341 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 2401 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 2461 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 2521 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 2581 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 2641 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 2701 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 2761 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 2821 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 2881 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 2941 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 3001 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 3061 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 3121 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 3181 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 3241 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 3301 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 3361 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 3421 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 3481 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 3541 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 3601 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 3661 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 3721 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 3781 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 3841 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 3901 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 3961 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 4021 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 4081 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 4141 gagcgcagcg agtcagtgag cgaggaagcg gaag //
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