出品公司: | Addgene |
---|---|
载体名称: | pMDLg/pRRE |
质粒类型: | 慢病毒系统;包装载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8895bp |
5' 测序引物及序列: | CMV Fwd: CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素(Ampicillian) |
筛选标记: | -- |
备注: | pMDLg/pRRE是第三代慢病毒包装载体。 |
产品目录号: | 12251 |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | 慢病毒 |
pMDLg/pRRE慢病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格:¥1920
- 货 号:BiovectorMDLg/pRRE
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
This 3rd generation LV packaging plasmid includes gag, coding for the virion main structural proteins;pol, responsible for the retrovirus-specific enzymes; and RRE, a binding site for the Rev protein whichfacilitates export of the RNA from the nucleus. This plasmid does not include Rev. The third generation packaging system offers maximal biosafety but is more cumbersome, as it involves the transfection of four different plasmids in the producer cells.Example of a 3rd generation packaging combo: pMDLg/pRRE + pRSV-Rev + pMD2.G.Please note that most Trono lab lentivectors contain a wt 5'LTR and can be packaged only using the 2nd generation packaging system (as the wt 5'LTR requires TAT for activation).If you wish to use the 3rd generation packaging system, you need to have a lentivector with a chimeric 5'LTR (e.g. CCL-, RRL-, etc) in which the HIV promoter is replaced with CMV or RSV, thus making it TAT-independent.The lentivectors carrying the chimeric 5'LTR can be packaged into either a 2nd or 3rd generation packaging system.
载体序列
载体质粒图谱pdf版和相关资料下载
载体应用举例
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