出品公司: | Clontech |
---|---|
载体名称: | pLVX-Puro , pLVXpuro |
质粒类型: | 哺乳动物细胞慢病毒表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 8102 bp |
5' 测序引物及序列: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄 |
筛选标记: | 嘌呤霉素 |
备注: | 含有组成型CMV启动子的慢病毒载体 |
产品目录号: | 632164 |
稳定性: | / |
组成型: | -- |
病毒/非病毒: | 慢病毒 |
pLVX-Puro慢病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格:¥1920
- 货 号:BiovectorLVX-Puro
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
pLVXpuro载体基本信息
pLVXpuro载体质粒图谱和多克隆位点信息
pLVXpuro载体简介
Description
pLVX-Puro is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to express your gene of interest in virtually any cell type, even primary cells. Expression of your gene is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the multiple cloning site (MCS), allowing constitutive, high level expression of your protein of interest.
pLVX-Puro contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (1), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (2). Finally, pLVX-Puro also contains a central polypurine tract (cPPT) element that increases nuclear importation of the viral genome during target cell infection, resulting in improved vector integration and more effi cient transduction (3). In addition to lentiviral elements, pLVX-Puro contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.
Use
pLVX-Puro constitutively expresses your gene of interest from PCMV IE when transduced into target cells. Before the vector can be transduced into cells, however, it must be transfected into 293T packaging cells with our Lenti-X™ HT Packaging System (Cat. Nos. 632160 and 632161). This packaging system allows you to safely produce high titer, infectious, replication-incompetent, VSV-G pseudotyped lentiviral particles that can infect a wide range of cell types, including non-dividing and primary cells (4).
pLVXpuro载体序列
LOCUS pLVX-Puro 8102 bp DNA circular SYN DEFINITION pLVX-Puro ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| FEATURES Location/Qualifiers source 1..8102 /organism="pLVX-Puro" /mol_type="other DNA" misc_feature 454..634 /label="HIV-1_5_LTR" misc_feature 454..634 /label="truncHIV-1_3_LTR" misc_feature 745..789 /label="HIV-1_psi_pack" CDS complement(1015..1476) /label="ORF frame 3" CDS 1181..2032 /label="ORF frame 2" misc_feature 1303..1536 /label="RRE" misc_feature 2031..2046 /label="cPPT" promoter 2198..2702 /label="CMV_immearly_promoter" misc_feature 2205..2492 /label="CAG_enhancer" misc_feature 2659..2679 /label="CMV_fwd_primer" promoter 2660..2729 /label="CMV_promoter" misc_feature 2703..2722 /label="pCEP_fwd_primer" misc_feature 2705..2729 /label="LNCX_primer" misc_feature complement(2925..2948) /label="MSCV_rev_primer" CDS 3170..4009 /label="ORF frame 2" misc_feature 3322..3341 /label="mPGK_F_primer" gene 3410..4009 /label="puro (variant)" /gene="puro (variant)" misc_feature 4025..4600 /label="WPRE" CDS 4113..4640 /label="ORF frame 3" misc_feature 5271..5451 /label="HIV-1_5_LTR" misc_feature 5271..5451 /label="truncHIV-1_3_LTR" promoter complement(5576..5594) /label="M13_reverse_primer" misc_feature complement(5593..5615) /label="M13_pUC_rev_primer" promoter complement(5629..5658) /label="lac_promoter" gene complement(6741..7601) /label="Ampicillin" /gene="Ampicillin" CDS complement(6741..7601) /label="ORF frame 1" promoter complement(7643..7671) /label="AmpR_promoter" terminator 7757..7888 /label="SV40_PA_terminator" misc_feature 7845..7864 /label="EBV_rev_primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagat 2881 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag 2941 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca 3001 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc 3061 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg 3121 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg 3181 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc 3241 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc 3301 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt 3361 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta 3421 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc 3481 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga 3541 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 3601 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 3661 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc 3721 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 3781 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 3841 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc 3901 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 3961 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac cgcgtctgga 4021 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg 4081 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc 4141 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt 4201 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca 4261 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc 4321 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc 4381 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc 4441 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc 4501 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc 4561 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta 4621 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac 4681 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac 4741 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 4801 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga 4861 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc 4921 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga 4981 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca 5041 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt 5101 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg 5161 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga 5221 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct 5281 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 5341 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 5401 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 5461 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 5521 ggccttgaca ttgctagcgt tttaccgtcg acctctagct agagcttggc gtaatcatgg 5581 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 5641 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 5701 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 5761 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 5821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 5881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 5941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 6001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 6061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 6121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 6181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 6241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 6301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 6361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 6421 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 6481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 6541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 6601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 6661 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 6721 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 6781 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 6841 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 6901 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 6961 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 7021 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 7081 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 7141 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 7201 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 7261 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 7321 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 7381 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 7441 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 7501 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 7561 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 7621 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 7681 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac 7741 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 7801 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 7861 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc 7921 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta 7981 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac 8041 tacaaactta gtagttttta aagaaattgt atttgttaaa tatgtactac aaacttagta 8101 gt //
pLVXpuro载体质粒图谱pdf版和相关资料下载
pLVXpuro载体应用举例
您正在向 biovector.net 发送关于产品 pLVX-Puro慢病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc. 的询问
- 公告/新闻