CHO EXP cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

CHO EXP cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
ExpiCHO™
Expression System
The ExpiCHO™ Expression System is designed to allow high-density transfection
of suspension ExpiCHO-S™ cells in a defined, serum-free medium. The system
includes ExpiCHO-S™ cells that have been adapted to serum-free, high-density
suspension culture in ExpiCHO™ Expression Medium. Transfection and
expression experiments may be performed directly in ExpiCHO™ Expression
Medium without the need for media change. The ExpiCHO™ Expression System
Kit provides cells, culture medium, and reagents to transfect 1 liter of cell culture,
as described below. Except for the ExpiCHO-S™ cells, all of the components of
the ExpiCHO™ Expression System are animal origin-free.
ExpiCHO-S™ Cells The ExpiCHO-S™ cell line is a clonal derivative of the CHO-S cell line. The
ExpiCHO-S™ cells are adapted to high-density suspension culture in ExpiCHO™
Expression Medium. Frozen cells are supplied in, and may be thawed directly
into, ExpiCHO™ Expression Medium.
The ExpiCHO-S™ cell line exhibits the following characteristics:
• Derived from the same parental lineage as the CHO-S cell line
• Rapid recovery post-thaw
• Adapted to high density, serum-free, suspension growth in ExpiCHO™
Expression Medium
• Doubling time of approximately 17 hours
• Reaches maximum cell densities of approximately 20 × 106 cells/mL in shake
flask culture
• High protein expression
• Stable transient expression over passages
The ExpiCHO-S™ cells are also available separately; see page 20 for ordering
information.
ExpiCHO™
Expression Medium
ExpiCHO™ Expression Medium is a chemically-defined medium developed
specifically for the high-density culture and transfection of ExpiCHO-S™ cells in
suspension. ExpiCHO™ Expression Medium requires no additional
supplementation.
ExpiCHO™ Expression Medium exhibits the following features:
• Formulated as an optimized, chemically-defined, serum-free, protein-free,
animal origin-free medium to support the high-density culture and
transfection of ExpiCHO-S™ cells in suspension.
• Supplemented with GlutaMAX™-I.
• Does not interfere with nor reduce the activity of ExpiFectamine™ CHO
Reagent.
• Designed for scalable transient transfection and protein expression
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net