TKE2 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心

TKE2 cell line细胞株细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell Line Name: TKE2


Keywords: Mouse cornea epithelial progenitor cells
Cell Line Description: TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.
Species: Mouse CD1
Tissue of Origin: Cornea

CellType: Epithelial; small and regular cobblestone-like shape

Growth Mode: Adherent
Karyotype: Normal karyotype of the female mouse CD1 strain


Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2 x 102 - 2 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
Culture Medium: Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2mM Glutamine.
Country: Japan
References: Kawakita T, Shimmura S, Hornia A, Higa K, Tseng SC. 2008 Stratified epithelial sheets engineered from a single adult murine corneal/limbal progenitor cell. J Cell Mol Med. 12(4):1303-16 PMID: 18318692.


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