rna E.coli mutant大肠杆菌基因缺失菌株-BioVector NTCC保藏中心

rna E.coli mutant大肠杆菌基因缺失菌株-BioVector NTCC保藏中心
Gene Symbol突变基因名称：rna
Mnemonic:RNaseA
Synonyms别称：rnsA
Gene product基因产物：RNase I
Selectable Phenotype筛选表型：unknown
Culture Condition培养条件：nutrient broth, Casamino acids or Casamino acids glucose medium (compositions described in Notes)
Other Phenotypes其他表型：
Notes：•nutrient broth containing per liter10 g of Bactotryptone (Difco), 1 g of yeast extract (Difco), 8 g of NaCl and 2 mM of CaCl₂,
•Casamino acids glycerol medium containing per liter 5 g of Casamino acids (Difco), 5 g of NaCl, 1 g of NH₄Cl, 4 ml of glycerol, 0.1 mM of Na₂HPO₄, 10 microM of FeCl₃, 1 mM of MgSO₄, 1.21 g of tris (Sigma 121) and 2 mM of CaCl₂
•Casamino acids glucose medium containing per liter 2 g of Casamino acids (Difco, vitamine-free), 1 g of NaCl, 2 g of dextrose, 2 g of (NH₄)₂SO₄, 20 ml of 1 M potassium phosphate buffer (pH 6.8) and 0.5 ml of salt solution containing 32 of MgCl₂, 0.6 g of CaCl₂ and 0.2 g of Fe₂(SO₄)₃ in 100 ml
Reference参考文献：Gessteland, R. F.
J. Mol. Biol. 1966. 16: 67-84

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