β-TC3 cell line细胞株beta-TC3，BETA-TC-3, b-TC3 BioVector NTCC质粒载体菌种细胞基因保藏中心

β-TC3 cell line细胞株beta-TC3，BETA-TC-3, b-TC3 BioVector NTCC质粒载体菌种细胞基因保藏中心
The pancreatic beta-cell line has been established from insulinomas derived from transgenic mice carrying a hybrid insulin-promoted simian virus 40 tumor antigen gene. The cells produce both proinsulin I and II and efficiently process each into mature insulin, in a manner comparable to normal beta cells in isolated islets. Insulin secretion is inducible up to 30-fold by glucose, although with a lower threshold for maximal stimulation than that for normal beta cells.
Cell line:BETA-TC-3NTCC no.:NTCC-DACC 324Species:murine (mouse) (Mus musculus)Cell type:pancreas insulinoma (beta-tumor)Origin:established from pancreatic insulinoma excised from a transgenic mouse carrying hybrid insulin-SV40 large T genes in 1988; described as producing pro-insulin I and II processed into mature insulin (inducible 30-fold by glucose); however, the NTCC did not verify the production of insulinBiosafety level:1Permissions and restrictions:A NTCC Cell Culture Data:Morphology:adherent cells growing as multilayers; Image:
Medium:82.5% Dulbecco's MEM (4.5 g/L glucose) + 15% horse serum + 2.5% h.i. FBSSubculture:split confluent culture 1:5 to 1:10 every 2-5 days by mechanical disruption (trypsin/EDTA can be used for a very short time); seed out at ca. 1-4 x 106 cells/175 cm2; the NTCC cannot guarantee the production of insulin.Incubation:at 37 °C with 7.5-10% CO2Doubling time:ca. 34 hoursHarvest:cell harvest of ca. 2-3 x 105 cells/cm2Storage:frozen with 70% medium, 20% FBS, 10% DMSO NTCC Scientific Data:Mycoplasma:negative in DAPI, microbiological culture, RNA hybridization assaysSpecies:originally confirmed as mouse with IEF of AST, NP and by species PCR and recently as mus musculus by COI DNA BarcodingCytogenetics:murine hypotetraploid karyotype - 74(70-78)<4n> - 2-4 centric fusion markers and 1-3 dmin presentViruses:ELISA: reverse transcriptase positive; PCR: SMRV -
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net