Beta-TC-6(小鼠胰岛素瘤胰岛β细胞) cell line BioVector NTCC质粒载体菌种细胞基因保藏中心

Beta-TC-6(小鼠胰岛素瘤胰岛β细胞) cell line BioVector NTCC质粒载体菌种细胞基因保藏中心
Beta-TC-6 ( CRL-11506) Organism: Mus musculus, transgenic for SV40 large T antigen, mouse, transgenic for SV40 large T antigen / Cell Type: beta cell / Tissue: pancreas / Disease: insulinoma
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Organism Mus musculus, transgenic for SV40 large T antigen, mouse, transgenic for SV40 large T antigen Tissue pancreas Cell Type beta cell Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 2 [Cells contain SV40 viral DNA Sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease insulinoma
Derivation The cell line was derived from a pancreatic tumor (insulinoma) arising in a transgenic mouse. Genes Expressed insulin, glucagon, and somatostatin Cellular Products insulin, glucagon and somatostatin Comments They secrete insulin in response to glucose. The mouse carried the pseudogene construct composed of the SV40 early region controlled by the rat insulin II gene promotor. The cells contain abundant insulin and small amounts of glucagon and somatostatin. They secrete insulin in response to glucose.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%. Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C
Deposited As mouse, transgenic for SV40 large T antigen U.S. Patent Number 6,455,292
【Supplier来源】BioVector NTCC Inc.
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