NS0-Turbodoma cell line小鼠骨髓瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

NS0-Turbodoma cell line小鼠骨髓瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Cell Line Name: NS0-Turbodoma
Keywords: Mouse myeloma, serum-free, without cholesterol
Cell Line Description: NS0 cell line adapted to grow in the chemically defined protein- and peptide-free medium Turbodoma . Cholesterol is not required as a supplement for this culture. This cell line is suitable for use as a fusion parent for serum-free generation of hybridomas.

Species: Mouse
Tissue of Origin: Myeloma
CellType: lymphoblast
Karyotype: Not specified

Subculture Routine: Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 105 cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO2; 37°C.
Culture Medium: Turbodoma medium  + 2mM Glutamine. When freezing cells down use TurboDoma medium (50:50 conditioned medium:fresh medium) + 2mM Glutamine + 10% DMSO.
Originator: Yes
Country: Switzerland

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