出品公司: | Clontech |
---|---|
载体名称: | pLVX-TRE3G-Luc Control , pLVX TRE3G Luc Contrtol |
质粒类型: | 哺乳动物细胞慢病毒表达载体, 阳性对照质粒 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | TRE3GV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 9452 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄 |
筛选标记: | 嘌呤霉素 |
备注: | 包含有Tet-On 3G反式激活蛋白,多西环素(doxycycline)诱导基因表达的慢病毒载体,是慢病毒表达的阳性对照质粒。 |
产品目录号: | 631187 |
稳定性: | / |
组成型: | 诱导型 |
病毒/非病毒: | 慢病毒 |
pLVX-TRE3G-Luc Control慢病毒表达载体质粒图谱序列抗性价格报价Biovector NTCC Inc.
- 价 格:¥1920
- 货 号:BiovectorLVX-TRE3G-Luc Control
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-TRE3G-Luc Control 9452 bp DNA circular 31-MAY-2011 COMMENT Created by Clontech Laboratories Inc. COMMENT 631191,631193,631187, 631189, 631191, 631193, 631360, 631361,631362,631187,631189,631349,631350,631351,631352,631353,631354,631355,631356,631357, 635087 COMMENT This file is created by Vector NTI http://www.biovector.net/ COMMENT VNTAUTHORNAME|biovector.net| FEATURES Location/Qualifiers misc_feature 1..635 /label=5'\LTR misc_feature 685..822 /label=Packaging\signal misc_feature 1303..1536 /label=RRE misc_feature 2028..2151 /label=cPPT misc_feature 4233..4736 /label=pPGK misc_feature 4757..5356 /label=Puro(R) misc_feature 5374..5965 /label=WPRE 3'clip 6169..6805 /label=3'\LTR misc_feature complement(7275..7945) /label=pUC\ori misc_feature 2566..4217 /label=Luciferase misc_feature 2210..2555 /label=pTRE3G misc_feature complement(8090..8950) /label=Amp(R) BASE COUNT 2452 a 2295 c 2424 g 2281 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat 2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac 2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga 2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat 2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag 2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc 2521 ctggagcaat tccacaacac ttttgtctta tacttggatc caccatggaa gacgccaaaa 2581 acataaagaa aggcccggcg ccattctatc cgctagagga tggaaccgct ggagagcaac 2641 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac 2701 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag 2761 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc 2821 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga 2881 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag 2941 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa 3001 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca 3061 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct 3121 ttgatcgtga caaaacaatt gcactgataa tgaactcctc tggatctact gggttaccta 3181 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta 3241 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg 3301 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt 3361 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt 3421 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt 3481 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag 3541 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta 3601 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg 3661 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc 3721 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg 3781 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact 3841 gggacgaaga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag 3901 gataccaggt ggcccccgct gaattggagt cgatattgtt acaacacccc aacatcttcg 3961 acgcgggcgt ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg 4021 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag 4081 taacaaccgc caaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc 4141 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg 4201 gaaagtccaa attgtaaaat acgcgtgaat tctaccgggt aggggaggcg cttttcccaa 4261 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc 4321 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg 4381 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc 4441 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg 4501 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt 4561 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg 4621 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc 4681 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct 4741 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 4801 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 4861 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 4921 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 4981 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 5041 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 5101 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 5161 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 5221 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 5281 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 5341 caagcccggt gcctgacggg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt 5401 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 5461 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 5521 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 5581 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 5641 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 5701 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 5761 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 5821 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 5881 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 5941 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg 6001 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac 6061 aagaggagga ggaggtgggt ttttccagtc acacctcagg tacctttaag accaatgact 6121 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta 6181 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac 6241 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga 6301 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag 6361 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg 6421 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg 6481 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt 6541 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa 6601 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct 6661 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 6721 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 6781 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 6841 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt 6901 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 6961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 7021 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 7081 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 7141 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 7201 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 7261 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 7321 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 7381 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 7441 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 7501 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 7561 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 7621 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 7681 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 7741 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 7801 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 7861 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 8041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 8101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 8161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 8221 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 8281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 8341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 8401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 8461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 8521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 8581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 8641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 9001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 9061 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct 9121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 9181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac 9241 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact 9301 gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt 9361 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt 9421 atttgttaaa tatgtactac aaacttagta gt //
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