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NCI-H2342 [H2342] cell line细胞株

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  • 货  号:NCI-H2342 [H2342] cell line细胞株
  • 产  地:北京
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NCI-H2342  [H2342] cell line
   Cat No.: NTCC-CRL5941

   OrganismHomo sapiens, human
   Product Formatfrozen
   Culture Propertiesadherent
   Biosafety Level1
   Diseasestage 3A, adenocarcinoma;  non-small cell lung cancer
   Age55 years

   DerivationThe line was established  in April 1990 from lung adenocarcinoma.
   Clinical Data55 years
   The tissue donor was a non-smoker.

   Complete Growth MediumHITES medium  supplemented with 5% fetal bovine serum
   The base medium for this cell line is DMEM:F12 Medium. To make the complete  growth medium,add the following components to the base medium
   1.0.005 mg/ml Insulin
   2.0.01 mg/ml Transferrin
   3.30nM Sodium selenite (final conc.)
   4.10 nM Hydrocortisone (final conc.)
   5.10 nM beta-estradiol (final conc.)
   6.extra 2mM L-glutamine (for final conc. of 4.5 mM)
   7.5% fetal bovine serum (final conc.)

   SubculturingVolumes used in this  protocol are for 75 cm2 flasks; proportionally reduce or increase amount of  dissociation medium for culture vessels of other sizes.
   1.Remove and discard culture medium.
   2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's  phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA  solution to remove all traces of serum which contains trypsin  inhibitor.
   3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells  under an inverted microscope until cell layer is dispersed (usually within 5  to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the  flask while waiting for the cells to detach. Cells that are difficult to  detach may be placed at 37°C to facilitate dispersal.
   4.Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently  pipetting
   5.Resuspend the cell pellet in fresh growth medium. Add appropriate  aliquots of the cell suspension to new culture vessels.
   6.Incubate cultures at 37°C.
   Subcultivation Ratio: 1:2 to 1:6.
   CryopreservationCulture medium,  95%; DMSO, 5%
   Culture ConditionsTemperature:  37°C

   STR ProfileAmelogenin: X,Y
   CSF1PO: 10
   D13S317: 12
   D16S539: 13
   D5S818: 12
   D7S820: 10
   TH01: 9,9.3
   TPOX: 8,10
   vWA: 17

   Year of Origin1990
   ReferencesNCI-Navy Medical Oncology  Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

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