首页 » LAN-1 cell line人神经母细胞瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

LAN-1 cell line人神经母细胞瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥99865
  • 货  号:LAN-1 cell line人神经母细胞瘤细胞株
  • 产  地:北京
点击询问我要采购
 竭诚为您服务!
BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.

电话:+86-010-53513060. 工作QQ:1843439339

邮件:Biovector@163.com

手机:18901268599

地址:北京

已注册
 

LAN-1 cell line人神经母细胞瘤细胞株
Cell line:LAN-1

DSMZ no.:ACC 655

Species:human (Homo sapiens)

Cell type:neuroblastoma

Origin:established in 1977 from a bone marrow metastasis of a 2-year-old boy with stage IV neuroblastoma

Biosafety level:1

Permissions and restrictions:A

DSMZ Cell Culture Data:

Morphology:cells are growing adherently and in clumps in suspension; image;

Medium:80-90% RPMI 1640 + 10-20% h.i. FBS or 80-90% MEM (with Earle's salts) + 10-20% h.i. FBS + non-essential amino acids + 2 mM L-glutamine

Subculture:seed out at ca. 1 x 106 cells/25 cm2 flask; split confluent culture 1:2 every 3-4 days using trypsin/EDTA (cells can also be detached by tapping the flask or by pipetting); viability can decrease shortly to 50-70% after seedingIncubation:at 37 °C with 5% CO2Doubling time:ca. 100 hoursHarvest:cell harvest of ca. 25 x 106 cells/175 cm2Storage:frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:Mycoplasma:contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays

Immunology:To inquire about expression of EpCAM and intermediate filaments, contact us.

Fingerprint:fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile

Viruses:PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -


Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net

您正在向 biovector.net  发送关于产品 LAN-1 cell line人神经母细胞瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 的询问

点击“立即发送”后,我们将在1个工作日内与您取得联系。