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NOMO-1 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

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  • 货  号:NOMO-1 cell line细胞株
  • 产  地:北京
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NOMO-1 cell line细胞株


Cell line:
NOMO-1
DSMZ no.:
ACC 542
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the bone marrow of a 31-year-old woman with acute myeloid leukemia (AML FAB M5a) at 2nd relapse; described in the literature to show strong lysozyme activity in the cytoplasm, phagocytic activities, and cells are responsive to induction of differentiation with TPA; cells carry the t(9;11)(p22;q23) KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) alteration
Reference(s):
14595, 15031, 14493, 15144, 16555
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
single round cells growing in suspension; image; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/ml; after thawing cell growth may be retarded; maintain at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 twice a week
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35 hours
Harvest:
cell harvest of ca. 1.5-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR +; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics and recently by STR fingerprinting
Cytogenetics:
human hyperdiploid karyotype with 8% polyploidy - 46-47<2n>XX, +8, -13, +mar, add(7)(q32), der(9)del(9)(p11p13-21)t(9;11)(p22;q23), t(9;13)(q13;q11), der(11)t(9;11)(p22;q23) - carries reciprocal t(9;11) with rearrangement of KMT2A (MLL) - matches published karyotype
Molec. Genetics:
expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -


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